Search results for the GEO ID: GSE16846  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM422108 | GPL339 |
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Mouse_Lung_Saline_14days_A
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Homogenized mouse lung, 14 days following intratracheal saline administration
|
strain: C57BL/6J
genotype: wildtype
gender: male
age: 8-10 weeks
tissue: lung
treatment: saline
|
Total RNA from homogenized mouse lung, 14 days following intratracheal saline administration, analysed using Affymetrix MOE430A GeneChips.
|
| Sample_geo_accession | GSM422108
| | Sample_status | Public on Jul 07 2009
| | Sample_submission_date | Jun 25 2009
| | Sample_last_update_date | Jul 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Dr Chris Scotton
| | Sample_treatment_protocol_ch1 | Bleomycin (1mg/kg body weight in 50ul of saline) or saline was administered by oropharyngeal installation as described previously by Lakatos et al., Exp Cell Res, 2006, under light halothane-induced anaesthesia. After 14 days, lungs were dissected, then immediately snap frozen in liquid nitrogen and stored at -80degC prior to use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen lung tissue was pulverized under liquid nitrogen using a pestle and mortar, then an aliquot of lung powder was immediately placed in Trizol (Invitrogen). Total RNA was extracted according to the manufacturer's instructions, and DNase-treated using DNAfree kit (Ambion). RNA quality was assessed using an Agilent Bioanalyser. Double-stranded cDNA was synthesized using the Superscript Double-Stranded cDNA synthesis kit (Invitrogen, UK) according to the manufacturer’s instructions, except that a T7-(dT)24 oligonucleotide (5’-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGT24-3') was used.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized using the BioArray High Efficiency RNA Transcript Labeling kit (Enzo Life Sciences, UK) according to the manufacturer's instructions and fragmented prior to microarray analysis.
| | Sample_hyb_protocol | Hybridization was performed according to the Affymetrix guidelines, using a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Scanning was performed according to the Affymetrix guidelines, using a GeneChip Scanner 3000.
| | Sample_data_processing | Processed data provided here are RMA expression values generated using RMAexpress software, with the default parameters (quartiles normalization and median polishing).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chris,,Scotton
| | Sample_contact_email | c.scotton@ucl.ac.uk
| | Sample_contact_phone | +442076796283
| | Sample_contact_laboratory | Centre for Respiratory Research
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University College London
| | Sample_contact_address | 5 University Street
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | WC1E 6JF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422108/suppl/GSM422108.cel.gz
| | Sample_series_id | GSE16846
| | Sample_data_row_count | 22690
| | |
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GSM422117 | GPL339 |
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Mouse_Lung_Saline_14days_B
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Homogenized mouse lung, 14 days following intratracheal saline administration
|
strain: C57BL/6J
genotype: wildtype
gender: male
age: 8-10 weeks
tissue: lung
treatment: saline
|
Total RNA from homogenized mouse lung, 14 days following intratracheal saline administration, analysed using Affymetrix MOE430A GeneChips.
|
| Sample_geo_accession | GSM422117
| | Sample_status | Public on Jul 07 2009
| | Sample_submission_date | Jun 25 2009
| | Sample_last_update_date | Jul 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Dr Chris Scotton
| | Sample_treatment_protocol_ch1 | Bleomycin (1mg/kg body weight in 50ul of saline) or saline was administered by oropharyngeal installation as described previously by Lakatos et al., Exp Cell Res, 2006, under light halothane-induced anaesthesia. After 14 days, lungs were dissected, then immediately snap frozen in liquid nitrogen and stored at -80degC prior to use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen lung tissue was pulverized under liquid nitrogen using a pestle and mortar, then an aliquot of lung powder was immediately placed in Trizol (Invitrogen). Total RNA was extracted according to the manufacturer's instructions, and DNase-treated using DNAfree kit (Ambion). RNA quality was assessed using an Agilent Bioanalyser. Double-stranded cDNA was synthesized using the Superscript Double-Stranded cDNA synthesis kit (Invitrogen, UK) according to the manufacturer’s instructions, except that a T7-(dT)24 oligonucleotide (5’-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGT24-3') was used.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized using the BioArray High Efficiency RNA Transcript Labeling kit (Enzo Life Sciences, UK) according to the manufacturer's instructions and fragmented prior to microarray analysis.
| | Sample_hyb_protocol | Hybridization was performed according to the Affymetrix guidelines, using a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Scanning was performed according to the Affymetrix guidelines, using a GeneChip Scanner 3000.
| | Sample_data_processing | Processed data provided here are RMA expression values generated using RMAexpress software, with the default parameters (quartiles normalization and median polishing).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chris,,Scotton
| | Sample_contact_email | c.scotton@ucl.ac.uk
| | Sample_contact_phone | +442076796283
| | Sample_contact_laboratory | Centre for Respiratory Research
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University College London
| | Sample_contact_address | 5 University Street
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | WC1E 6JF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422117/suppl/GSM422117.cel.gz
| | Sample_series_id | GSE16846
| | Sample_data_row_count | 22690
| | |
|
GSM422231 | GPL339 |
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Mouse_Lung_Saline_14days_C
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Homogenized mouse lung, 14 days following intratracheal saline administration
|
strain: C57BL/6J
genotype: wildtype
gender: male
age: 8-10 weeks
tissue: lung
treatment: saline
|
Total RNA from homogenized mouse lung, 14 days following intratracheal saline administration, analysed using Affymetrix MOE430A GeneChips.
|
| Sample_geo_accession | GSM422231
| | Sample_status | Public on Jul 07 2009
| | Sample_submission_date | Jun 25 2009
| | Sample_last_update_date | Jul 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Bleomycin (1mg/kg body weight in 50ul of saline) or saline was administered by oropharyngeal installation as described previously by Lakatos et al., Exp Cell Res, 2006, under light halothane-induced anaesthesia. After 14 days, lungs were dissected, then immediately snap frozen in liquid nitrogen and stored at -80degC prior to use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen lung tissue was pulverized under liquid nitrogen using a pestle and mortar, then an aliquot of lung powder was immediately placed in Trizol (Invitrogen). Total RNA was extracted according to the manufacturer's instructions, and DNase-treated using DNAfree kit (Ambion). RNA quality was assessed using an Agilent Bioanalyser. Double-stranded cDNA was synthesized using the Superscript Double-Stranded cDNA synthesis kit (Invitrogen, UK) according to the manufacturer’s instructions, except that a T7-(dT)24 oligonucleotide (5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGT24-3') was used.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized using the BioArray High Efficiency RNA Transcript Labeling kit (Enzo Life Sciences, UK) according to the manufacturer's instructions and fragmented prior to microarray analysis.
| | Sample_hyb_protocol | Hybridization was performed according to the Affymetrix guidelines, using a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Scanning was performed according to the Affymetrix guidelines, using a GeneChip Scanner 3000.
| | Sample_data_processing | Processed data provided here are RMA expression values generated using RMAexpress software, with the default parameters (quartiles normalization and median polishing).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chris,,Scotton
| | Sample_contact_email | c.scotton@ucl.ac.uk
| | Sample_contact_phone | +442076796283
| | Sample_contact_laboratory | Centre for Respiratory Research
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University College London
| | Sample_contact_address | 5 University Street
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | WC1E 6JF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422231/suppl/GSM422231.cel.gz
| | Sample_series_id | GSE16846
| | Sample_data_row_count | 22690
| | |
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GSM422308 | GPL339 |
|
Mouse_Lung_Bleomycin_14days_D
|
Homogenized mouse lung, 14 days following intratracheal Bleomycin administration
|
strain: C57BL/6J
genotype: wildtype
gender: male
age: 8-10 weeks
tissue: lung
treatment: Bleomycin
|
Total RNA from homogenized mouse lung, 14 days following intratracheal Bleomycin administration, analysed using Affymetrix MOE430A GeneChips.
|
| Sample_geo_accession | GSM422308
| | Sample_status | Public on Jul 07 2009
| | Sample_submission_date | Jun 26 2009
| | Sample_last_update_date | Jul 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Bleomycin (1mg/kg body weight in 50ul of saline) or saline was administered by oropharyngeal installation as described previously by Lakatos et al., Exp Cell Res, 2006, under light halothane-induced anaesthesia. After 14 days, lungs were dissected, then immediately snap frozen in liquid nitrogen and stored at -80degC prior to use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen lung tissue was pulverized under liquid nitrogen using a pestle and mortar, then an aliquot of lung powder was immediately placed in Trizol (Invitrogen). Total RNA was extracted according to the manufacturer's instructions, and DNase-treated using DNAfree kit (Ambion). RNA quality was assessed using an Agilent Bioanalyser. Double-stranded cDNA was synthesized using the Superscript Double-Stranded cDNA synthesis kit (Invitrogen, UK) according to the manufacturer’s instructions, except that a T7-(dT)24 oligonucleotide (5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGT24-3') was used.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized using the BioArray High Efficiency RNA Transcript Labeling kit (Enzo Life Sciences, UK) according to the manufacturer's instructions and fragmented prior to microarray analysis.
| | Sample_hyb_protocol | Hybridization was performed according to the Affymetrix guidelines, using a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Scanning was performed according to the Affymetrix guidelines, using a GeneChip Scanner 3000.
| | Sample_data_processing | Processed data provided here are RMA expression values generated using RMAexpress software, with the default parameters (quartiles normalization and median polishing).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chris,,Scotton
| | Sample_contact_email | c.scotton@ucl.ac.uk
| | Sample_contact_phone | +442076796283
| | Sample_contact_laboratory | Centre for Respiratory Research
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University College London
| | Sample_contact_address | 5 University Street
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | WC1E 6JF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422308/suppl/GSM422308.cel.gz
| | Sample_series_id | GSE16846
| | Sample_data_row_count | 22690
| | |
|
GSM422309 | GPL339 |
|
Mouse_Lung_Bleomycin_14days_E
|
Homogenized mouse lung, 14 days following intratracheal Bleomycin administration
|
strain: C57BL/6J
genotype: wildtype
gender: male
age: 8-10 weeks
tissue: lung
treatment: Bleomycin
|
Total RNA from homogenized mouse lung, 14 days following intratracheal Bleomycin administration, analysed using Affymetrix MOE430A GeneChips.
|
| Sample_geo_accession | GSM422309
| | Sample_status | Public on Jul 07 2009
| | Sample_submission_date | Jun 26 2009
| | Sample_last_update_date | Jul 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Bleomycin (1mg/kg body weight in 50ul of saline) or saline was administered by oropharyngeal installation as described previously by Lakatos et al., Exp Cell Res, 2006, under light halothane-induced anaesthesia. After 14 days, lungs were dissected, then immediately snap frozen in liquid nitrogen and stored at -80degC prior to use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen lung tissue was pulverized under liquid nitrogen using a pestle and mortar, then an aliquot of lung powder was immediately placed in Trizol (Invitrogen). Total RNA was extracted according to the manufacturer's instructions, and DNase-treated using DNAfree kit (Ambion). RNA quality was assessed using an Agilent Bioanalyser. Double-stranded cDNA was synthesized using the Superscript Double-Stranded cDNA synthesis kit (Invitrogen, UK) according to the manufacturer’s instructions, except that a T7-(dT)24 oligonucleotide (5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGT24-3') was used.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized using the BioArray High Efficiency RNA Transcript Labeling kit (Enzo Life Sciences, UK) according to the manufacturer's instructions and fragmented prior to microarray analysis.
| | Sample_hyb_protocol | Hybridization was performed according to the Affymetrix guidelines, using a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Scanning was performed according to the Affymetrix guidelines, using a GeneChip Scanner 3000.
| | Sample_data_processing | Processed data provided here are RMA expression values generated using RMAexpress software, with the default parameters (quartiles normalization and median polishing).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chris,,Scotton
| | Sample_contact_email | c.scotton@ucl.ac.uk
| | Sample_contact_phone | +442076796283
| | Sample_contact_laboratory | Centre for Respiratory Research
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University College London
| | Sample_contact_address | 5 University Street
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | WC1E 6JF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422309/suppl/GSM422309.cel.gz
| | Sample_series_id | GSE16846
| | Sample_data_row_count | 22690
| | |
|
GSM422310 | GPL339 |
|
Mouse_Lung_Bleomycin_14days_F
|
Homogenized mouse lung, 14 days following intratracheal Bleomycin administration
|
strain: C57BL/6J
genotype: wildtype
gender: male
age: 8-10 weeks
tissue: lung
treatment: Bleomycin
|
Total RNA from homogenized mouse lung, 14 days following intratracheal Bleomycin administration, analysed using Affymetrix MOE430A GeneChips.
|
| Sample_geo_accession | GSM422310
| | Sample_status | Public on Jul 07 2009
| | Sample_submission_date | Jun 26 2009
| | Sample_last_update_date | Jul 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Bleomycin (1mg/kg body weight in 50ul of saline) or saline was administered by oropharyngeal installation as described previously by Lakatos et al., Exp Cell Res, 2006, under light halothane-induced anaesthesia. After 14 days, lungs were dissected, then immediately snap frozen in liquid nitrogen and stored at -80degC prior to use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen lung tissue was pulverized under liquid nitrogen using a pestle and mortar, then an aliquot of lung powder was immediately placed in Trizol (Invitrogen). Total RNA was extracted according to the manufacturer's instructions, and DNase-treated using DNAfree kit (Ambion). RNA quality was assessed using an Agilent Bioanalyser. Double-stranded cDNA was synthesized using the Superscript Double-Stranded cDNA synthesis kit (Invitrogen, UK) according to the manufacturer’s instructions, except that a T7-(dT)24 oligonucleotide (5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGT24-3') was used.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized using the BioArray High Efficiency RNA Transcript Labeling kit (Enzo Life Sciences, UK) according to the manufacturer's instructions and fragmented prior to microarray analysis.
| | Sample_hyb_protocol | Hybridization was performed according to the Affymetrix guidelines, using a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Scanning was performed according to the Affymetrix guidelines, using a GeneChip Scanner 3000.
| | Sample_data_processing | Processed data provided here are RMA expression values generated using RMAexpress software, with the default parameters (quartiles normalization and median polishing).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chris,,Scotton
| | Sample_contact_email | c.scotton@ucl.ac.uk
| | Sample_contact_phone | +442076796283
| | Sample_contact_laboratory | Centre for Respiratory Research
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University College London
| | Sample_contact_address | 5 University Street
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | WC1E 6JF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422310/suppl/GSM422310.cel.gz
| | Sample_series_id | GSE16846
| | Sample_data_row_count | 22690
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