Result

Search results for the GEO ID: GSE16854

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM422406

GPL339

ctrl_rep1

UGS mesenchyme cells, control

strain: CD-1 gender: male developmental stage: gestation day 17 tissue: prostate cell type: urogenital sinus mesenchyme treatment: control

Cultured fetal prostate mesenchyme cells.

Sample_geo_accession	GSM422406
Sample_status	Public on Jun 27 2009
Sample_submission_date	Jun 26 2009
Sample_last_update_date	Jun 26 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	First passage cells were seeded at 0.315 x 10e6 cells/well in 35 mm dishes, in the same RPMI medium with endogenous hormones removed by substituting 5 % (v/v) charcoal-stripped FBS and 5 % (v/v) charcoal-stripped horse serum (Sigma, St. Louis, MO) for the 10% whole FBS, and further supplementing with ITS supplement, for final concentrations of 10 μg insulin/ml, 10 μg transferrin/ml, and 10 ng selenium/ml. Cells were maintained in a constant background of 690 pM DHT (200 pg/ml) during treatment with estradiol. Cells were maintained in estrogen-free medium for three days, with one medium change, before the start of estrogen treatment, and then treated with 100 nM estradiol for four days with daily medium changes. At the end of the treatment period the cells were washed once with PBS, and immediately lysed on ice in Trizol.
Sample_growth_protocol_ch1	Fetal UGS tissue was disrupted by collagenase treatment. Epithelial cells were discarded, and the collected mesenchymal cells were cultured at 37oC under 5% CO2 in RPMI-1640 without phenol red, supplemented with 2 mM L-glutamine, 100 units penicillin G sodium/ml, 100 mg streptomycin sulfate/ml, and 0.25 mg fungizone/ml, with 10% (v/v) fetal bovine serum (FBS). Cells were grown to 95 % confluence, and then passaged by digestion with 0.05 % trypsin in 0.53 mM EDTA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the Trizol lysate and purified with the RNeasy Mini kit according to the manufacturers’ instructions. RNA quality was checked on an Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA preparations were used to synthesize double-stranded cDNA using the Affymetrix One-Cycle cDNA Synthesis kit, and then amplified and biotinylated using the GeneChip IVT Labeling kit.
Sample_hyb_protocol	cRNA was purified and fragmented using the GeneChip Sample Cleanup Module, and then hybridized to the Affymetrix mouse genome 430A array. Quality control, hybridization, washing and scanning were performed according to the GeneChip Expression Analysis Technical Manual.
Sample_scan_protocol	Scanning was performed with an Agilent microarray scanner.
Sample_data_processing	Scanned data were analyzed with Affymetrix GeneChip Operating Software.
Sample_platform_id	GPL339
Sample_contact_name	Julia,A,Taylor
Sample_contact_department	Biological Sciences
Sample_contact_institute	University of Missouri
Sample_contact_address	114 Lefevre Hall
Sample_contact_city	Columbia
Sample_contact_state	MO
Sample_contact_zip/postal_code	65211
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422406/suppl/GSM422406.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422406/suppl/GSM422406.CHP.gz
Sample_series_id	GSE16854
Sample_data_row_count	22690

GSM422407

GPL339

ctrl_rep2

UGS mesenchyme cells, control

strain: CD-1 gender: male developmental stage: gestation day 17 tissue: prostate cell type: urogenital sinus mesenchyme treatment: control

Cultured fetal prostate mesenchyme cells.

Sample_geo_accession	GSM422407
Sample_status	Public on Jun 27 2009
Sample_submission_date	Jun 26 2009
Sample_last_update_date	Jun 26 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	First passage cells were seeded at 0.315 x 10e6 cells/well in 35 mm dishes, in the same RPMI medium with endogenous hormones removed by substituting 5 % (v/v) charcoal-stripped FBS and 5 % (v/v) charcoal-stripped horse serum (Sigma, St. Louis, MO) for the 10% whole FBS, and further supplementing with ITS supplement, for final concentrations of 10 μg insulin/ml, 10 μg transferrin/ml, and 10 ng selenium/ml. Cells were maintained in a constant background of 690 pM DHT (200 pg/ml) during treatment with estradiol. Cells were maintained in estrogen-free medium for three days, with one medium change, before the start of estrogen treatment, and then treated with 100 nM estradiol for four days with daily medium changes. At the end of the treatment period the cells were washed once with PBS, and immediately lysed on ice in Trizol.
Sample_growth_protocol_ch1	Fetal UGS tissue was disrupted by collagenase treatment. Epithelial cells were discarded, and the collected mesenchymal cells were cultured at 37oC under 5% CO2 in RPMI-1640 without phenol red, supplemented with 2 mM L-glutamine, 100 units penicillin G sodium/ml, 100 mg streptomycin sulfate/ml, and 0.25 mg fungizone/ml, with 10% (v/v) fetal bovine serum (FBS). Cells were grown to 95 % confluence, and then passaged by digestion with 0.05 % trypsin in 0.53 mM EDTA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the Trizol lysate and purified with the RNeasy Mini kit according to the manufacturers’ instructions. RNA quality was checked on an Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA preparations were used to synthesize double-stranded cDNA using the Affymetrix One-Cycle cDNA Synthesis kit, and then amplified and biotinylated using the GeneChip IVT Labeling kit.
Sample_hyb_protocol	cRNA was purified and fragmented using the GeneChip Sample Cleanup Module, and then hybridized to the Affymetrix mouse genome 430A array. Quality control, hybridization, washing and scanning were performed according to the GeneChip Expression Analysis Technical Manual.
Sample_scan_protocol	Scanning was performed with an Agilent microarray scanner.
Sample_data_processing	Scanned data were analyzed with Affymetrix GeneChip Operating Software.
Sample_platform_id	GPL339
Sample_contact_name	Julia,A,Taylor
Sample_contact_department	Biological Sciences
Sample_contact_institute	University of Missouri
Sample_contact_address	114 Lefevre Hall
Sample_contact_city	Columbia
Sample_contact_state	MO
Sample_contact_zip/postal_code	65211
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422407/suppl/GSM422407.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422407/suppl/GSM422407.CHP.gz
Sample_series_id	GSE16854
Sample_data_row_count	22690

GSM422408

GPL339

ctrl_rep3

UGS mesenchyme cells, control

strain: CD-1 gender: male developmental stage: gestation day 17 tissue: prostate cell type: urogenital sinus mesenchyme treatment: control

Cultured fetal prostate mesenchyme cells.

Sample_geo_accession	GSM422408
Sample_status	Public on Jun 27 2009
Sample_submission_date	Jun 26 2009
Sample_last_update_date	Jun 26 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	First passage cells were seeded at 0.315 x 10e6 cells/well in 35 mm dishes, in the same RPMI medium with endogenous hormones removed by substituting 5 % (v/v) charcoal-stripped FBS and 5 % (v/v) charcoal-stripped horse serum (Sigma, St. Louis, MO) for the 10% whole FBS, and further supplementing with ITS supplement, for final concentrations of 10 μg insulin/ml, 10 μg transferrin/ml, and 10 ng selenium/ml. Cells were maintained in a constant background of 690 pM DHT (200 pg/ml) during treatment with estradiol. Cells were maintained in estrogen-free medium for three days, with one medium change, before the start of estrogen treatment, and then treated with 100 nM estradiol for four days with daily medium changes. At the end of the treatment period the cells were washed once with PBS, and immediately lysed on ice in Trizol.
Sample_growth_protocol_ch1	Fetal UGS tissue was disrupted by collagenase treatment. Epithelial cells were discarded, and the collected mesenchymal cells were cultured at 37oC under 5% CO2 in RPMI-1640 without phenol red, supplemented with 2 mM L-glutamine, 100 units penicillin G sodium/ml, 100 mg streptomycin sulfate/ml, and 0.25 mg fungizone/ml, with 10% (v/v) fetal bovine serum (FBS). Cells were grown to 95 % confluence, and then passaged by digestion with 0.05 % trypsin in 0.53 mM EDTA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the Trizol lysate and purified with the RNeasy Mini kit according to the manufacturers’ instructions. RNA quality was checked on an Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA preparations were used to synthesize double-stranded cDNA using the Affymetrix One-Cycle cDNA Synthesis kit, and then amplified and biotinylated using the GeneChip IVT Labeling kit.
Sample_hyb_protocol	cRNA was purified and fragmented using the GeneChip Sample Cleanup Module, and then hybridized to the Affymetrix mouse genome 430A array. Quality control, hybridization, washing and scanning were performed according to the GeneChip Expression Analysis Technical Manual.
Sample_scan_protocol	Scanning was performed with an Agilent microarray scanner.
Sample_data_processing	Scanned data were analyzed with Affymetrix GeneChip Operating Software.
Sample_platform_id	GPL339
Sample_contact_name	Julia,A,Taylor
Sample_contact_department	Biological Sciences
Sample_contact_institute	University of Missouri
Sample_contact_address	114 Lefevre Hall
Sample_contact_city	Columbia
Sample_contact_state	MO
Sample_contact_zip/postal_code	65211
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422408/suppl/GSM422408.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422408/suppl/GSM422408.CHP.gz
Sample_series_id	GSE16854
Sample_data_row_count	22690

GSM422409

GPL339

E2_rep1

UGS mesenchyme cells, estradiol-treated

strain: CD-1 gender: male developmental stage: gestation day 17 tissue: prostate cell type: urogenital sinus mesenchyme treatment: estradiol

Cultured fetal prostate mesenchyme cells.

Sample_geo_accession	GSM422409
Sample_status	Public on Jun 27 2009
Sample_submission_date	Jun 26 2009
Sample_last_update_date	Jun 26 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	First passage cells were seeded at 0.315 x 10e6 cells/well in 35 mm dishes, in the same RPMI medium with endogenous hormones removed by substituting 5 % (v/v) charcoal-stripped FBS and 5 % (v/v) charcoal-stripped horse serum (Sigma, St. Louis, MO) for the 10% whole FBS, and further supplementing with ITS supplement, for final concentrations of 10 μg insulin/ml, 10 μg transferrin/ml, and 10 ng selenium/ml. Cells were maintained in a constant background of 690 pM DHT (200 pg/ml) during treatment with estradiol. Cells were maintained in estrogen-free medium for three days, with one medium change, before the start of estrogen treatment, and then treated with 100 nM estradiol for four days with daily medium changes. At the end of the treatment period the cells were washed once with PBS, and immediately lysed on ice in Trizol.
Sample_growth_protocol_ch1	Fetal UGS tissue was disrupted by collagenase treatment. Epithelial cells were discarded, and the collected mesenchymal cells were cultured at 37oC under 5% CO2 in RPMI-1640 without phenol red, supplemented with 2 mM L-glutamine, 100 units penicillin G sodium/ml, 100 mg streptomycin sulfate/ml, and 0.25 mg fungizone/ml, with 10% (v/v) fetal bovine serum (FBS). Cells were grown to 95 % confluence, and then passaged by digestion with 0.05 % trypsin in 0.53 mM EDTA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the Trizol lysate and purified with the RNeasy Mini kit according to the manufacturers’ instructions. RNA quality was checked on an Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA preparations were used to synthesize double-stranded cDNA using the Affymetrix One-Cycle cDNA Synthesis kit, and then amplified and biotinylated using the GeneChip IVT Labeling kit.
Sample_hyb_protocol	cRNA was purified and fragmented using the GeneChip Sample Cleanup Module, and then hybridized to the Affymetrix mouse genome 430A array. Quality control, hybridization, washing and scanning were performed according to the GeneChip Expression Analysis Technical Manual.
Sample_scan_protocol	Scanning was performed with an Agilent microarray scanner.
Sample_data_processing	Scanned data were analyzed with Affymetrix GeneChip Operating Software.
Sample_platform_id	GPL339
Sample_contact_name	Julia,A,Taylor
Sample_contact_department	Biological Sciences
Sample_contact_institute	University of Missouri
Sample_contact_address	114 Lefevre Hall
Sample_contact_city	Columbia
Sample_contact_state	MO
Sample_contact_zip/postal_code	65211
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422409/suppl/GSM422409.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422409/suppl/GSM422409.CHP.gz
Sample_series_id	GSE16854
Sample_data_row_count	22690

GSM422410

GPL339

E2_rep2

UGS mesenchyme cells, estradiol-treated

strain: CD-1 gender: male developmental stage: gestation day 17 tissue: prostate cell type: urogenital sinus mesenchyme treatment: estradiol

Cultured fetal prostate mesenchyme cells.

Sample_geo_accession	GSM422410
Sample_status	Public on Jun 27 2009
Sample_submission_date	Jun 26 2009
Sample_last_update_date	Jun 26 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	First passage cells were seeded at 0.315 x 10e6 cells/well in 35 mm dishes, in the same RPMI medium with endogenous hormones removed by substituting 5 % (v/v) charcoal-stripped FBS and 5 % (v/v) charcoal-stripped horse serum (Sigma, St. Louis, MO) for the 10% whole FBS, and further supplementing with ITS supplement, for final concentrations of 10 μg insulin/ml, 10 μg transferrin/ml, and 10 ng selenium/ml. Cells were maintained in a constant background of 690 pM DHT (200 pg/ml) during treatment with estradiol. Cells were maintained in estrogen-free medium for three days, with one medium change, before the start of estrogen treatment, and then treated with 100 nM estradiol for four days with daily medium changes. At the end of the treatment period the cells were washed once with PBS, and immediately lysed on ice in Trizol.
Sample_growth_protocol_ch1	Fetal UGS tissue was disrupted by collagenase treatment. Epithelial cells were discarded, and the collected mesenchymal cells were cultured at 37oC under 5% CO2 in RPMI-1640 without phenol red, supplemented with 2 mM L-glutamine, 100 units penicillin G sodium/ml, 100 mg streptomycin sulfate/ml, and 0.25 mg fungizone/ml, with 10% (v/v) fetal bovine serum (FBS). Cells were grown to 95 % confluence, and then passaged by digestion with 0.05 % trypsin in 0.53 mM EDTA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the Trizol lysate and purified with the RNeasy Mini kit according to the manufacturers’ instructions. RNA quality was checked on an Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA preparations were used to synthesize double-stranded cDNA using the Affymetrix One-Cycle cDNA Synthesis kit, and then amplified and biotinylated using the GeneChip IVT Labeling kit.
Sample_hyb_protocol	cRNA was purified and fragmented using the GeneChip Sample Cleanup Module, and then hybridized to the Affymetrix mouse genome 430A array. Quality control, hybridization, washing and scanning were performed according to the GeneChip Expression Analysis Technical Manual.
Sample_scan_protocol	Scanning was performed with an Agilent microarray scanner.
Sample_data_processing	Scanned data were analyzed with Affymetrix GeneChip Operating Software.
Sample_platform_id	GPL339
Sample_contact_name	Julia,A,Taylor
Sample_contact_department	Biological Sciences
Sample_contact_institute	University of Missouri
Sample_contact_address	114 Lefevre Hall
Sample_contact_city	Columbia
Sample_contact_state	MO
Sample_contact_zip/postal_code	65211
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422410/suppl/GSM422410.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422410/suppl/GSM422410.CHP.gz
Sample_series_id	GSE16854
Sample_data_row_count	22690

GSM422411

GPL339

E2_rep3

UGS mesenchyme cells, estradiol-treated

strain: CD-1 gender: male developmental stage: gestation day 17 tissue: prostate cell type: urogenital sinus mesenchyme treatment: estradiol

Cultured fetal prostate mesenchyme cells.

Sample_geo_accession	GSM422411
Sample_status	Public on Jun 27 2009
Sample_submission_date	Jun 26 2009
Sample_last_update_date	Jun 26 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	First passage cells were seeded at 0.315 x 10e6 cells/well in 35 mm dishes, in the same RPMI medium with endogenous hormones removed by substituting 5 % (v/v) charcoal-stripped FBS and 5 % (v/v) charcoal-stripped horse serum (Sigma, St. Louis, MO) for the 10% whole FBS, and further supplementing with ITS supplement, for final concentrations of 10 μg insulin/ml, 10 μg transferrin/ml, and 10 ng selenium/ml. Cells were maintained in a constant background of 690 pM DHT (200 pg/ml) during treatment with estradiol. Cells were maintained in estrogen-free medium for three days, with one medium change, before the start of estrogen treatment, and then treated with 100 nM estradiol for four days with daily medium changes. At the end of the treatment period the cells were washed once with PBS, and immediately lysed on ice in Trizol.
Sample_growth_protocol_ch1	Fetal UGS tissue was disrupted by collagenase treatment. Epithelial cells were discarded, and the collected mesenchymal cells were cultured at 37oC under 5% CO2 in RPMI-1640 without phenol red, supplemented with 2 mM L-glutamine, 100 units penicillin G sodium/ml, 100 mg streptomycin sulfate/ml, and 0.25 mg fungizone/ml, with 10% (v/v) fetal bovine serum (FBS). Cells were grown to 95 % confluence, and then passaged by digestion with 0.05 % trypsin in 0.53 mM EDTA.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the Trizol lysate and purified with the RNeasy Mini kit according to the manufacturers’ instructions. RNA quality was checked on an Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA preparations were used to synthesize double-stranded cDNA using the Affymetrix One-Cycle cDNA Synthesis kit, and then amplified and biotinylated using the GeneChip IVT Labeling kit.
Sample_hyb_protocol	cRNA was purified and fragmented using the GeneChip Sample Cleanup Module, and then hybridized to the Affymetrix mouse genome 430A array. Quality control, hybridization, washing and scanning were performed according to the GeneChip Expression Analysis Technical Manual.
Sample_scan_protocol	Scanning was performed with an Agilent microarray scanner.
Sample_data_processing	Scanned data were analyzed with Affymetrix GeneChip Operating Software.
Sample_platform_id	GPL339
Sample_contact_name	Julia,A,Taylor
Sample_contact_department	Biological Sciences
Sample_contact_institute	University of Missouri
Sample_contact_address	114 Lefevre Hall
Sample_contact_city	Columbia
Sample_contact_state	MO
Sample_contact_zip/postal_code	65211
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422411/suppl/GSM422411.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422411/suppl/GSM422411.CHP.gz
Sample_series_id	GSE16854
Sample_data_row_count	22690

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