Search results for the GEO ID: GSE16870  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM422830 | GPL570 |
|
6 hr control (BafA) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422830
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422830/suppl/GSM422830.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422830/suppl/GSM422830.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422831 | GPL570 |
|
6 hr 15 nM BafA 1 rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422831
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422831/suppl/GSM422831.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422831/suppl/GSM422831.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422832 | GPL570 |
|
6 hr control (BafA) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422832
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422832/suppl/GSM422832.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422832/suppl/GSM422832.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422833 | GPL570 |
|
6 hr 15 nM BafA 1 rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422833
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422833/suppl/GSM422833.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422833/suppl/GSM422833.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422834 | GPL570 |
|
6 hr control (DFO) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422834
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422834/suppl/GSM422834.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422834/suppl/GSM422834.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422835 | GPL570 |
|
6 hr 100 µM DFO rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422835
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422835/suppl/GSM422835.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422835/suppl/GSM422835.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422836 | GPL570 |
|
6 hr control (DFO) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422836
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422836/suppl/GSM422836.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422836/suppl/GSM422836.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422837 | GPL570 |
|
6 hr 100 µM DFO rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422837
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422837/suppl/GSM422837.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422837/suppl/GSM422837.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422838 | GPL570 |
|
6 hr control (LDL) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422838
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422838/suppl/GSM422838.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422838/suppl/GSM422838.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422839 | GPL570 |
|
6 hr low LDL rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422839
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422839/suppl/GSM422839.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422839/suppl/GSM422839.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422840 | GPL570 |
|
6 hr control (LDL) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422840
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422840/suppl/GSM422840.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422840/suppl/GSM422840.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422841 | GPL570 |
|
6 hr low LDL rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422841
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422841/suppl/GSM422841.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422841/suppl/GSM422841.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422842 | GPL570 |
|
12 hr control (BafA) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422842
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422842/suppl/GSM422842.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422842/suppl/GSM422842.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422843 | GPL570 |
|
12 hr 15 nM BafA 1 rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422843
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422843/suppl/GSM422843.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422843/suppl/GSM422843.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422844 | GPL570 |
|
12 hr control (BafA) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422844
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422844/suppl/GSM422844.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422844/suppl/GSM422844.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422845 | GPL570 |
|
12 hr 15 nM BafA 1 rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422845
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422845/suppl/GSM422845.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422845/suppl/GSM422845.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422846 | GPL570 |
|
12 hr control (DFO) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422846
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422846/suppl/GSM422846.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422846/suppl/GSM422846.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422847 | GPL570 |
|
12 hr 100 µM DFO rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422847
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422847/suppl/GSM422847.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422847/suppl/GSM422847.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422848 | GPL570 |
|
12 hr control (DFO) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422848
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422848/suppl/GSM422848.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422848/suppl/GSM422848.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422849 | GPL570 |
|
12 hr 100 µM DFO rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422849
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422849/suppl/GSM422849.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422849/suppl/GSM422849.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422850 | GPL570 |
|
12 hr control (LDL) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422850
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422850/suppl/GSM422850.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422850/suppl/GSM422850.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422851 | GPL570 |
|
12 hr low LDL rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422851
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422851/suppl/GSM422851.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422851/suppl/GSM422851.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422852 | GPL570 |
|
12 hr control (LDL) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422852
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422852/suppl/GSM422852.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422852/suppl/GSM422852.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422853 | GPL570 |
|
12 hr low LDL rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422853
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422853/suppl/GSM422853.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422853/suppl/GSM422853.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422854 | GPL570 |
|
12 hr control (LX1077) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422854
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422854/suppl/GSM422854.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422854/suppl/GSM422854.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422855 | GPL570 |
|
12 hr 200 nM LX1077 rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422855
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422855/suppl/GSM422855.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422855/suppl/GSM422855.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422856 | GPL570 |
|
12 hr control (LX1077) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422856
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422856/suppl/GSM422856.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422856/suppl/GSM422856.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422857 | GPL570 |
|
12 hr 200 nM LX1077 rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422857
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422857/suppl/GSM422857.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422857/suppl/GSM422857.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422858 | GPL570 |
|
24 hr control (BafA) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422858
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422858/suppl/GSM422858.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422858/suppl/GSM422858.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422859 | GPL570 |
|
24 hr 15 nM BafA 1 rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422859
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422859/suppl/GSM422859.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422859/suppl/GSM422859.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422860 | GPL570 |
|
24 hr control (BafA) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422860
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422860/suppl/GSM422860.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422860/suppl/GSM422860.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422861 | GPL570 |
|
24 hr 15 nM BafA 1 rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422861
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422861/suppl/GSM422861.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422861/suppl/GSM422861.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422862 | GPL570 |
|
24 hr control (LX1077) rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422862
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422862/suppl/GSM422862.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422862/suppl/GSM422862.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422863 | GPL570 |
|
24 hr 200 nM LX1077 rep1
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422863
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422863/suppl/GSM422863.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422863/suppl/GSM422863.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422864 | GPL570 |
|
24 hr control (LX1077) rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422864
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422864/suppl/GSM422864.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422864/suppl/GSM422864.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
GSM422865 | GPL570 |
|
24 hr 200 nM LX1077 rep2
|
HeLa
|
cell line: HeLa
|
Gene expression data from HeLa cells incubated in the reagent listed for the time shown.
|
| Sample_geo_accession | GSM422865
| | Sample_status | Public on Jul 22 2010
| | Sample_submission_date | Jun 28 2009
| | Sample_last_update_date | Jul 22 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reagents were added at the concentrations and for the times listed on each sample. All samples exept the low LDL and matched controls received 0.1% DMSO.
| | Sample_growth_protocol_ch1 | Actively growing HeLa cells were plated in 10 cm diameter dishes in DMEM containing 10% fetal calf serum and grown overnight to just reach confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For each sample total RNA was isolated with RNeasy Mini Kit and at least 2 µg RNA was delivered to the UT Southwestern Microarray Core Facility.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA according to UT Southwestern Microarray protocol http://microarray.swmed.edu/AffyHybProtocol.htm.
| | Sample_hyb_protocol | Hybridization to Affymetriz Human Genome U133 Plus 2.0 Arrays was conducted according to the manufacturers instructions (http://microarray.swmed.edu/AffyHybProtocol.htm).
| | Sample_scan_protocol | Arrays were scanned with an Affy GeneChip 3000 7G according to the manufacturer's instructions.
| | Sample_data_processing | Data was processed with GCOS using MAS5.0 normalization to produce CHP files.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,G,Roth
| | Sample_contact_email | michael.roth@utsouthwestern.edu
| | Sample_contact_phone | 214 648 3276
| | Sample_contact_fax | 214 648 0314
| | Sample_contact_laboratory | Roth
| | Sample_contact_department | Biochemistry
| | Sample_contact_institute | UT Southwestern
| | Sample_contact_address | 5323 Harry Hines Blvd
| | Sample_contact_city | Dallas
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 75390-9038
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422865/suppl/GSM422865.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM422nnn/GSM422865/suppl/GSM422865.CHP.gz
| | Sample_series_id | GSE16870
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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