Search results for the GEO ID: GSE16962  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM424759 | GPL570 |
|
pSUPER-scramble replicate 1
|
HUVEC infected by retroviral vectors bearing a control scramble sequence
|
cell line: HUVEC
|
Gene expression data from HUVEC over-expressing a scramble control sequence
|
| Sample_geo_accession | GSM424759
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424759/suppl/GSM424759.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424760 | GPL570 |
|
pSUPER-scramble replicate 2
|
HUVEC infected by retroviral vectors bearing a control scramble sequence
|
cell line: HUVEC
|
Gene expression data from HUVEC over-expressing a scramble control sequence
|
| Sample_geo_accession | GSM424760
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424760/suppl/GSM424760.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424761 | GPL570 |
|
pSUPER-scramble replicate 3
|
HUVEC infected by retroviral vectors bearing a control scramble sequence
|
cell line: HUVEC
|
Gene expression data from HUVEC over-expressing a scramble control sequence
|
| Sample_geo_accession | GSM424761
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424761/suppl/GSM424761.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424762 | GPL570 |
|
pSUPER-mir-210 replicate 1
|
HUVEC infected by retroviral vectors bearing pre-miR-210 sequence
|
cell line: HUVEC
|
Gene expression data from HUVEC over-expressing miR-210
|
| Sample_geo_accession | GSM424762
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424762/suppl/GSM424762.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424763 | GPL570 |
|
pSUPER-mir-210 replicate 2
|
HUVEC infected by retroviral vectors bearing pre-miR-210 sequence
|
cell line: HUVEC
|
Gene expression data from HUVEC over-expressing miR-210
|
| Sample_geo_accession | GSM424763
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424763/suppl/GSM424763.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424764 | GPL570 |
|
pSUPER-mir-210 replicate 3
|
HUVEC infected by retroviral vectors bearing pre-miR-210 sequence
|
cell line: HUVEC
|
Gene expression data from HUVEC over-expressing miR-210
|
| Sample_geo_accession | GSM424764
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424764/suppl/GSM424764.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424765 | GPL570 |
|
Anti-scramble replicate 1
|
HUVEC transfected with a scramble-LNA
|
cell line: HUVEC
|
Gene expression data from HUVEC transfected with a scramble-LNA
|
| Sample_geo_accession | GSM424765
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424765/suppl/GSM424765.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424766 | GPL570 |
|
Anti-scramble replicate 2
|
HUVEC transfected with a scramble-LNA
|
cell line: HUVEC
|
Gene expression data from HUVEC transfected with a scramble-LNA
|
| Sample_geo_accession | GSM424766
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424766/suppl/GSM424766.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424767 | GPL570 |
|
Anti-scramble replicate 3
|
HUVEC transfected with a scramble-LNA
|
cell line: HUVEC
|
Gene expression data from HUVEC transfected with a scramble-LNA
|
| Sample_geo_accession | GSM424767
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424767/suppl/GSM424767.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424768 | GPL570 |
|
Anti-mir-210 replicate 1
|
HUVEC transfected with anti-miR-210-LNA
|
cell line: HUVEC
|
Gene expression data from HUVEC in which miR-210 activity was blocked by a complementary anti-miR-210-LNA
|
| Sample_geo_accession | GSM424768
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424768/suppl/GSM424768.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424769 | GPL570 |
|
Anti-mir-210 replicate 2
|
HUVEC transfected with anti-miR-210-LNA
|
cell line: HUVEC
|
Gene expression data from HUVEC in which miR-210 activity was blocked by a complementary anti-miR-210-LNA
|
| Sample_geo_accession | GSM424769
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424769/suppl/GSM424769.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
|
GSM424770 | GPL570 |
|
Anti-mir-210 replicate 3
|
HUVEC transfected with anti-miR-210-LNA
|
cell line: HUVEC
|
Gene expression data from HUVEC in which miR-210 activity was blocked by a complementary anti-miR-210-LNA
|
| Sample_geo_accession | GSM424770
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Jul 06 2009
| | Sample_last_update_date | Oct 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To obtain stable miR-210 over-expressing cells, HUVEC were infected by retroviral vectors bearing pre-miR-210 sequence or a control scramble sequence. To inhibit miR-210, Locked Nucleic Acid (LNA) oligonucleotides against miR-210 or a control scramble sequence (Exiqon) were transfected by siRNA Transfection Reagent (Santa Cruz), following manufacturer instructions, in 40% confluent HUVEC (4X103/cm2) at the final concentration of 40 nM.
| | Sample_growth_protocol_ch1 | HUVEC were grown in EGM-2 (Lonza) containing 2% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2.1sq. GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Summarized signal intensities were generated in BRB ArrayTools using justRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mario,,Pescatori
| | Sample_contact_email | mariopescatori@gmail.com
| | Sample_contact_phone | 00393930761013
| | Sample_contact_laboratory | experimental surgical oncology
| | Sample_contact_department | surgical oncology
| | Sample_contact_institute | Erasmusmc
| | Sample_contact_address | 50, molenwaterplain
| | Sample_contact_city | rotterdam
| | Sample_contact_zip/postal_code | 5000CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM424nnn/GSM424770/suppl/GSM424770.CEL.gz
| | Sample_series_id | GSE16962
| | Sample_data_row_count | 54675
| | |
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