Search results for the GEO ID: GSE17023  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM425416 | GPL1261 |
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32Dcl3 pMIG
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pMIG retorovirus vector transfected 32Dcl3 cells
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cell type: 32Dcl3
transfectant type: pMIG (pMY) retrovirus vector
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in situ oligonucleotide
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| Sample_geo_accession | GSM425416
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Jul 08 2009
| | Sample_last_update_date | May 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The retrovirus vector-plasmid was transfected into Plat-E packaging cells (a gift from Dr. Toshio Kitamura) using lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. 1 x 10e6 32Dcl3 cells were added to retroviral stock with 6 ug/ml of polybrene (Sigma) and spun at 1,400 g for 2 hours. After 24 hours, cells were harvested.
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% heat-inactivated fetal bovine serum, 10% mouse Interleukin-3 (10ng/ml) at 37.0°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was obtained using the RNeasy kit (Qiagen) according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | 5ug of cRNA was hybridized for 16 hours using standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol (Gene Chip Scanner 3000) was used.
| | Sample_data_processing | MAS5 Statistical Algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Morito,,Kurata
| | Sample_contact_email | kurata.pth2@tmd.ac.jp
| | Sample_contact_department | Comprehensive Patho.
| | Sample_contact_institute | Tokyo Med. & Dent. univ.
| | Sample_contact_address | bunkyo-ku yusima1-5-45
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-8519
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM425nnn/GSM425416/suppl/GSM425416.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM425nnn/GSM425416/suppl/GSM425416.CHP.gz
| | Sample_series_id | GSE17023
| | Sample_data_row_count | 45101
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GSM425417 | GPL1261 |
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32Dcl3 Xbp1U
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pMY-Xbp1U retrovirus vector transfected 32Dcl3 cells
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cell type: 32Dcl3
transfectant type: pMY-Xbp1U retrovirus vector
genome/variation: Xbp1U (unspliced form)
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in situ oligonucleotide
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| Sample_geo_accession | GSM425417
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Jul 08 2009
| | Sample_last_update_date | May 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The retrovirus vector-plasmid was transfected into Plat-E packaging cells (a gift from Dr. Toshio Kitamura) using lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. 1 x 10e6 32Dcl3 cells were added to retroviral stock with 6 ug/ml of polybrene (Sigma) and spun at 1,400 g for 2 hours. After 24 hours, cells were harvested.
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% heat-inactivated fetal bovine serum, 10% mouse Interleukin-3 (10ng/ml) at 37.0°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was obtained using the RNeasy kit (Qiagen) according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | 5ug of cRNA was hybridized for 16 hours using standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol (Gene Chip Scanner 3000) was used.
| | Sample_data_processing | MAS5 Statistical Algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Morito,,Kurata
| | Sample_contact_email | kurata.pth2@tmd.ac.jp
| | Sample_contact_department | Comprehensive Patho.
| | Sample_contact_institute | Tokyo Med. & Dent. univ.
| | Sample_contact_address | bunkyo-ku yusima1-5-45
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-8519
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM425nnn/GSM425417/suppl/GSM425417.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM425nnn/GSM425417/suppl/GSM425417.CHP.gz
| | Sample_series_id | GSE17023
| | Sample_data_row_count | 45101
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GSM425429 | GPL1261 |
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32Dcl3 Xbp1S
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pMY-Xbp1S retrovirus transfected 32Dcl3 cells
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cell type: 32Dcl3
transfectant type: pMY-Xbp1S retrovirus vector
genome/variation: Xbp1S (spliced form)
|
in situ oligonucleotide
|
| Sample_geo_accession | GSM425429
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Jul 08 2009
| | Sample_last_update_date | May 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The retrovirus vector-plasmid was transfected into Plat-E packaging cells (a gift from Dr. Toshio Kitamura) using lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. 1 x 10e6 32Dcl3 cells were added to retroviral stock with 6 ug/ml of polybrene (Sigma) and spun at 1,400 g for 2 hours. After 24 hours, cells were harvested.
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 10% heat-inactivated fetal bovine serum, 10% mouse Interleukin-3 (10ng/ml) at 37.0°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was obtained using the RNeasy kit (Qiagen) according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | 5ug of cRNA was hybridized for 16 hours using standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol (Gene Chip Scanner 3000) was used.
| | Sample_data_processing | MAS5 Statistical Algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Morito,,Kurata
| | Sample_contact_email | kurata.pth2@tmd.ac.jp
| | Sample_contact_department | Comprehensive Patho.
| | Sample_contact_institute | Tokyo Med. & Dent. univ.
| | Sample_contact_address | bunkyo-ku yusima1-5-45
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-8519
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM425nnn/GSM425429/suppl/GSM425429.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM425nnn/GSM425429/suppl/GSM425429.CHP.gz
| | Sample_series_id | GSE17023
| | Sample_data_row_count | 45101
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