Search results for the GEO ID: GSE17076 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM427234 | GPL1261 |
|
NSCL61-1
|
mouse NSC
|
strain: C57BL/6
genome/variation: p53-/-
developmental stage: E14.5 embryo
tissue: brain
cell type: Neural Stem Cells, Glioma Initiating Cell-like
cell line: NSCL61
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NSC-L61-cont-1_M430_2_08080101.CEL
|
Sample_geo_accession | GSM427234
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Jul 14 2009
| Sample_last_update_date | Jul 14 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Neuroepithelial cells were prepared from embryonic day 14.5 mouse telencephalon and expanded in serum-free Dulbecco's Modified Eagle's medium (DMEM)/F12 medium containing bovine insulin (10 μg/ml), human transferrin (100 μg/ml), BSA (100 μg/ml), progesterone (60 ng/ml), putrescine (16 μg/ml), sodium selenite (40 ng/ml), N-acetylcysteine (60 μg/ml), forskolin (5 μM), PDGF (10 ng/ml), penicillin, streptomycin, and bFGF as floating spheres.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Five micrograms of total RNA were labeled with biotin by in vitro transcription (IVT) using the One-Cycle Target Labeling procedure for biotin labeling by in vitro transcription (Affymetrix) according to the manufacturer’s instruction.
| Sample_hyb_protocol | Labeled probes were hybridized to the GeneChip Mouse Genome 430 2.0 array (Affymetrix) according to the manufacturer’s instructions.
| Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data.
| Sample_data_processing | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data. The CEL data were then analyzed with dChip software, which can normalize and process multiple datasets simultaneously.
| Sample_platform_id | GPL1261
| Sample_contact_name | Toru,,Kondo
| Sample_contact_email | tkondo@cdb.riken.jp
| Sample_contact_phone | +81-78-306-3170
| Sample_contact_fax | +81-78-306-3171
| Sample_contact_laboratory | Laboratory for Cell Lineage Modulation
| Sample_contact_department | Center for Developmental Biology
| Sample_contact_institute | RIKEN
| Sample_contact_address | 2-2-3, Minatojima-Minamimachi, Chuo-ku
| Sample_contact_city | Kobe
| Sample_contact_zip/postal_code | 650-0047
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427234/suppl/GSM427234.CEL.gz
| Sample_series_id | GSE17076
| Sample_series_id | GSE17101
| Sample_data_row_count | 45101
| |
|
GSM427235 | GPL1261 |
|
NSCL61-2
|
mouse NSC
|
strain: C57BL/6
genome/variation: p53-/-
developmental stage: E14.5 embryo
tissue: brain
cell type: Neural Stem Cells, Glioma Initiating Cell-like
cell line: NSCL61
|
NSC-L61-cont-2_M430_2_08080102.CEL
|
Sample_geo_accession | GSM427235
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Jul 14 2009
| Sample_last_update_date | Jul 14 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Neuroepithelial cells were prepared from embryonic day 14.5 mouse telencephalon and expanded in serum-free Dulbecco's Modified Eagle's medium (DMEM)/F12 medium containing bovine insulin (10 μg/ml), human transferrin (100 μg/ml), BSA (100 μg/ml), progesterone (60 ng/ml), putrescine (16 μg/ml), sodium selenite (40 ng/ml), N-acetylcysteine (60 μg/ml), forskolin (5 μM), PDGF (10 ng/ml), penicillin, streptomycin, and bFGF as floating spheres.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Five micrograms of total RNA were labeled with biotin by in vitro transcription (IVT) using the One-Cycle Target Labeling procedure for biotin labeling by in vitro transcription (Affymetrix) according to the manufacturer’s instruction.
| Sample_hyb_protocol | Labeled probes were hybridized to the GeneChip Mouse Genome 430 2.0 array (Affymetrix) according to the manufacturer’s instructions.
| Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data.
| Sample_data_processing | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data. The CEL data were then analyzed with dChip software, which can normalize and process multiple datasets simultaneously.
| Sample_platform_id | GPL1261
| Sample_contact_name | Toru,,Kondo
| Sample_contact_email | tkondo@cdb.riken.jp
| Sample_contact_phone | +81-78-306-3170
| Sample_contact_fax | +81-78-306-3171
| Sample_contact_laboratory | Laboratory for Cell Lineage Modulation
| Sample_contact_department | Center for Developmental Biology
| Sample_contact_institute | RIKEN
| Sample_contact_address | 2-2-3, Minatojima-Minamimachi, Chuo-ku
| Sample_contact_city | Kobe
| Sample_contact_zip/postal_code | 650-0047
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427235/suppl/GSM427235.CEL.gz
| Sample_series_id | GSE17076
| Sample_series_id | GSE17101
| Sample_data_row_count | 45101
| |
|
GSM427236 | GPL1261 |
|
NSCL61-sox11-1
|
mouse NSC
|
strain: C57BL/6
genome/variation: p53-/-, sox11-expressing
developmental stage: E14.5 embryo
tissue: brain
cell type: Neural Stem Cells, Glioma Initiating Cell-like
cell line: NSCL61-sox11
|
NSC-L61-s11-314-1_M430_2_08080103.CEL
|
Sample_geo_accession | GSM427236
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Jul 14 2009
| Sample_last_update_date | Jul 14 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Neuroepithelial cells were prepared from embryonic day 14.5 mouse telencephalon and expanded in serum-free Dulbecco's Modified Eagle's medium (DMEM)/F12 medium containing bovine insulin (10 μg/ml), human transferrin (100 μg/ml), BSA (100 μg/ml), progesterone (60 ng/ml), putrescine (16 μg/ml), sodium selenite (40 ng/ml), N-acetylcysteine (60 μg/ml), forskolin (5 μM), PDGF (10 ng/ml), penicillin, streptomycin, and bFGF as floating spheres.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Five micrograms of total RNA were labeled with biotin by in vitro transcription (IVT) using the One-Cycle Target Labeling procedure for biotin labeling by in vitro transcription (Affymetrix) according to the manufacturer’s instruction.
| Sample_hyb_protocol | Labeled probes were hybridized to the GeneChip Mouse Genome 430 2.0 array (Affymetrix) according to the manufacturer’s instructions.
| Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data.
| Sample_data_processing | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data. The CEL data were then analyzed with dChip software, which can normalize and process multiple datasets simultaneously.
| Sample_platform_id | GPL1261
| Sample_contact_name | Toru,,Kondo
| Sample_contact_email | tkondo@cdb.riken.jp
| Sample_contact_phone | +81-78-306-3170
| Sample_contact_fax | +81-78-306-3171
| Sample_contact_laboratory | Laboratory for Cell Lineage Modulation
| Sample_contact_department | Center for Developmental Biology
| Sample_contact_institute | RIKEN
| Sample_contact_address | 2-2-3, Minatojima-Minamimachi, Chuo-ku
| Sample_contact_city | Kobe
| Sample_contact_zip/postal_code | 650-0047
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427236/suppl/GSM427236.CEL.gz
| Sample_series_id | GSE17076
| Sample_series_id | GSE17101
| Sample_data_row_count | 45101
| |
|
GSM427237 | GPL1261 |
|
NSCL61-sox11-2
|
mouse NSC
|
strain: C57BL/6
genome/variation: p53-/-, sox11-expressing
developmental stage: E14.5 embryo
tissue: brain
cell type: Neural Stem Cells, Glioma Initiating Cell-like
cell line: NSCL61-sox11
|
NSC-L61-s11-314-2_M430_2_08080104.CEL
|
Sample_geo_accession | GSM427237
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Jul 14 2009
| Sample_last_update_date | Jul 14 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Neuroepithelial cells were prepared from embryonic day 14.5 mouse telencephalon and expanded in serum-free Dulbecco's Modified Eagle's medium (DMEM)/F12 medium containing bovine insulin (10 μg/ml), human transferrin (100 μg/ml), BSA (100 μg/ml), progesterone (60 ng/ml), putrescine (16 μg/ml), sodium selenite (40 ng/ml), N-acetylcysteine (60 μg/ml), forskolin (5 μM), PDGF (10 ng/ml), penicillin, streptomycin, and bFGF as floating spheres.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Five micrograms of total RNA were labeled with biotin by in vitro transcription (IVT) using the One-Cycle Target Labeling procedure for biotin labeling by in vitro transcription (Affymetrix) according to the manufacturer’s instruction.
| Sample_hyb_protocol | Labeled probes were hybridized to the GeneChip Mouse Genome 430 2.0 array (Affymetrix) according to the manufacturer’s instructions.
| Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data.
| Sample_data_processing | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data. The CEL data were then analyzed with dChip software, which can normalize and process multiple datasets simultaneously.
| Sample_platform_id | GPL1261
| Sample_contact_name | Toru,,Kondo
| Sample_contact_email | tkondo@cdb.riken.jp
| Sample_contact_phone | +81-78-306-3170
| Sample_contact_fax | +81-78-306-3171
| Sample_contact_laboratory | Laboratory for Cell Lineage Modulation
| Sample_contact_department | Center for Developmental Biology
| Sample_contact_institute | RIKEN
| Sample_contact_address | 2-2-3, Minatojima-Minamimachi, Chuo-ku
| Sample_contact_city | Kobe
| Sample_contact_zip/postal_code | 650-0047
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427237/suppl/GSM427237.CEL.gz
| Sample_series_id | GSE17076
| Sample_series_id | GSE17101
| Sample_data_row_count | 45101
| |
|
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