Search results for the GEO ID: GSE17100  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM427816 | GPL96 |
|
day 4, control replicate 1
|
control replicate 1, day 4 in culture
|
condition: control
replicate: 1
time point: day 4 in culture
tissue: mononuclear hematopoietic cells
|
day 4, control replicate 1
|
| Sample_geo_accession | GSM427816
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427816/suppl/GSM427816.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427817 | GPL96 |
|
day 4, control replicate 2
|
control replicate 2, day 4 in culture
|
condition: control
replicate: 2
time point: day 4 in culture
tissue: mononuclear hematopoietic cells
|
day 4, control replicate 2
|
| Sample_geo_accession | GSM427817
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427817/suppl/GSM427817.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427818 | GPL96 |
|
day 4, PRAME replicate 1
|
PRAME replicate 1, day 4 in culture
|
condition: PRAME
replicate: 1
time point: day 4 in culture
tissue: mononuclear hematopoietic cells
|
day 4, PRAME replicate 1
|
| Sample_geo_accession | GSM427818
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427818/suppl/GSM427818.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427819 | GPL96 |
|
day 4, PRAME replicate 2
|
PRAME replicate 2, day 4 in culture
|
condition: PRAME
replicate: 2
time point: day 4 in culture
tissue: mononuclear hematopoietic cells
|
day 4, PRAME replicate 2
|
| Sample_geo_accession | GSM427819
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427819/suppl/GSM427819.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427820 | GPL96 |
|
day 7, control replicate 1
|
control replicate 1, day 7 in culture
|
condition: control
replicate: 1
time point: day 7 in culture
tissue: mononuclear hematopoietic cells
|
day 7, control replicate 1
|
| Sample_geo_accession | GSM427820
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427820/suppl/GSM427820.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427821 | GPL96 |
|
day 7, control replicate 2
|
control replicate 2, day 7 in culture
|
condition: control
replicate: 2
time point: day 7 in culture
tissue: mononuclear hematopoietic cells
|
day 7, control replicate 2
|
| Sample_geo_accession | GSM427821
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427821/suppl/GSM427821.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427822 | GPL96 |
|
day 7, PRAME replicate 1
|
PRAME replicate 1, day 7 in culture
|
condition: PRAME
replicate: 1
time point: day 7 in culture
tissue: mononuclear hematopoietic cells
|
day 7, PRAME replicate 1
|
| Sample_geo_accession | GSM427822
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427822/suppl/GSM427822.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427823 | GPL96 |
|
day 7, PRAME replicate 2
|
PRAME replicate 2, day 7 in culture
|
condition: PRAME
replicate: 2
time point: day 7 in culture
tissue: mononuclear hematopoietic cells
|
day 7, PRAME replicate 2
|
| Sample_geo_accession | GSM427823
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427823/suppl/GSM427823.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427824 | GPL96 |
|
day 14, control replicate 1
|
control replicate 1, day 14 in culture
|
condition: control
replicate: 1
time point: day 14 in culture
tissue: mononuclear hematopoietic cells
|
day 14, control replicate 1
|
| Sample_geo_accession | GSM427824
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427824/suppl/GSM427824.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427825 | GPL96 |
|
day 14, control replicate 2
|
control replicate 2, day 14 in culture
|
condition: control
replicate: 2
time point: day 14 in culture
tissue: mononuclear hematopoietic cells
|
day 14, control replicate 2
|
| Sample_geo_accession | GSM427825
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427825/suppl/GSM427825.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427826 | GPL96 |
|
day 14, PRAME replicate 1
|
PRAME replicate 1, day 14 in culture
|
condition: PRAME
replicate: 1
time point: day 14 in culture
tissue: mononuclear hematopoietic cells
|
day 14, PRAME replicate 1
|
| Sample_geo_accession | GSM427826
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427826/suppl/GSM427826.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
GSM427827 | GPL96 |
|
day 14, PRAME replicate 2
|
PRAME replicate 2, day 14 in culture
|
condition: PRAME
replicate: 2
time point: day 14 in culture
tissue: mononuclear hematopoietic cells
|
day 14, PRAME replicate 2
|
| Sample_geo_accession | GSM427827
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Jul 15 2009
| | Sample_last_update_date | Jul 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells for each day were spun down and resuspended in Trizol.
| | Sample_growth_protocol_ch1 | Hematopoietic cells from peripheral blood stem cell products from healthy donors without malignancy were thawed and cultured overnight in media containing Iscove's Modified Dulbecco's Medium (IMDM), 20% FBS, 1% penicillin/streptomycin/ fungisome, 10-4 M beta mercaptoethanol, 100 ng/ml of stem cell factor (SCF), 30 ng/ml IL3, and 50 ng/ml IL6. The following day the cells were transduced with either lentiviral vectors containing PRAME or the control vector. A double transduction protocol was used. After the second transduction cells were resuspended in media containing X-vivo 15, 1% lot-tested BSA, 2mM glutamine, 10-4 M beta mercaptoethanol, 100 ng/ml of SCF, 30 ng/ml IL3, and 50 ng/ml IL6. Cells were sorted using GFP fluorescence after transduction on Day 0. To examine the effects of PRAME protein expression on in vitro myeloid differentiation, after GFP selection, cells were cultured in 100 ng/ml of SCF, 30 ng/ml IL3, 20 ng/ml IL6, 20 ng/ml G-CSF, and 20 ng/ml GM-CSF and total RNA was extracted on days 4, 7, and 14.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of high quality RNA was amplified using a single-stranded linear amplification protocol (SLAP). This protocol is published (PMID 14706461). Biotin-labeling was performed using the Enzo BioArrayTM High Yield RNA Transcript Labeling Kit (Axxora, LLC, San Diego, CA).
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols and guidelines were followed as published in the GeneChipTM Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HU-133A). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | Image (DAT), cell intensity (CEL), and chip (CHP) files were generated using MAS 5.0 software (Affymetrix, Santa Clara, CA). Individual arrays were screened for quality.
| | Sample_data_processing | Expression values for individual probe sets were extracted from CEL files using the Bioconductor and gcRMA programs to background adjust, normalize and log-transform the data.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Vivian ,G.,Oehler
| | Sample_contact_email | voehler@u.washington.edu
| | Sample_contact_phone | (206) 667-1340
| | Sample_contact_fax | (206) 667-2917
| | Sample_contact_department | Clinical Research Division
| | Sample_contact_institute | Fred Hutchinson Cancer Research Center
| | Sample_contact_address | 1100 Fairview Ave. North, D4-100
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM427nnn/GSM427827/suppl/GSM427827.CEL.gz
| | Sample_series_id | GSE17100
| | Sample_data_row_count | 22283
| | |
|
| |
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