Search results for the GEO ID: GSE17129  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM428956 | GPL570 |
|
Ramos pRTS-GFP A+
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BL Ramos cell line
|
transfection: pRTS-GFP
cell type: human Burkitt lymphoma
dsmz no.: ACC 603
origin: established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described to be EBV-negative but EBV infectible, to carry the (8;14) translocation and p53 mutations
|
NA
|
| Sample_geo_accession | GSM428956
| | Sample_status | Public on Jul 22 2009
| | Sample_submission_date | Jul 16 2009
| | Sample_last_update_date | Jul 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Ramos cells were transfected in triplicates with pRTS-GFP or pRTS-CA-IKK2, selected with hygromycin and transgene expression was induced with doxycycline (0,5mg/ml) for 48hrs. RNA was isolated with RNeasy mini kit (Qiagen, Venlo, Netherlands)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | extraction with RNeasy Mini Kit (QIAGEN) according to manufacturer´s instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2 µg of total RNA were labeled using the GeneChip® One-Cycle Target Labeling assay kit (Affymetrix), according to the manufacturer´s instructions
| | Sample_hyb_protocol | Hybridization on a Hybridization Oven 640 (Affymetrix).
| | Sample_hyb_protocol | Arrays were stained and washed in a FS 450 Fluidics station (Affymetrix)
| | Sample_scan_protocol | Imaging on an Affymetrix GeneChip (3000) scanner. Raw data were generated using the GCOS 1.4 software (Affymetrix). Probe level data were obtained using the Robust Multichip Average (RMA) normalization algorithm [Bolstad, B.M.,
| | Sample_scan_protocol | Irizarry, R.A., Astrand, M. & Speed, T.P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185?193 (2003)]
| | Sample_data_processing | Data pre-processed with RMA.
| | Sample_data_processing | Data analysis was performed using the BRB-ARRAYTOOLS software package (available at http://linus.nci.nih.gov/BRB-ArrayTools.html)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kay,,Klapproth
| | Sample_contact_email | kay.klapproth@uni-ulm.de
| | Sample_contact_phone | +49 (0) 731 50033834
| | Sample_contact_fax | +49 (0) 731 5002 28924
| | Sample_contact_department | Institute of Physiological Chemistry
| | Sample_contact_institute | Ulm University
| | Sample_contact_address | Albert-Einstein-Allee 11
| | Sample_contact_city | Ulm
| | Sample_contact_zip/postal_code | 89081
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM428nnn/GSM428956/suppl/GSM428956.CEL.gz
| | Sample_series_id | GSE17129
| | Sample_data_row_count | 54675
| | |
|
GSM428957 | GPL570 |
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Ramos pRTS-GFP C+
|
BL Ramos cell line
|
transfection: pRTS-GFP
cell type: human Burkitt lymphoma
dsmz no.: ACC 603
origin: established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described to be EBV-negative but EBV infectible, to carry the (8;14) translocation and p53 mutations
|
NA
|
| Sample_geo_accession | GSM428957
| | Sample_status | Public on Jul 22 2009
| | Sample_submission_date | Jul 16 2009
| | Sample_last_update_date | Jul 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Ramos cells were transfected in triplicates with pRTS-GFP or pRTS-CA-IKK2, selected with hygromycin and transgene expression was induced with doxycycline (0,5mg/ml) for 48hrs. RNA was isolated with RNeasy mini kit (Qiagen, Venlo, Netherlands)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | extraction with RNeasy Mini Kit (QIAGEN) according to manufacturer´s instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2 µg of total RNA were labeled using the GeneChip® One-Cycle Target Labeling assay kit (Affymetrix), according to the manufacturer´s instructions
| | Sample_hyb_protocol | Hybridization on a Hybridization Oven 640 (Affymetrix).
| | Sample_hyb_protocol | Arrays were stained and washed in a FS 450 Fluidics station (Affymetrix)
| | Sample_scan_protocol | Imaging on an Affymetrix GeneChip (3000) scanner. Raw data were generated using the GCOS 1.4 software (Affymetrix). Probe level data were obtained using the Robust Multichip Average (RMA) normalization algorithm [Bolstad, B.M.,
| | Sample_scan_protocol | Irizarry, R.A., Astrand, M. & Speed, T.P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185?193 (2003)]
| | Sample_data_processing | Data pre-processed with RMA.
| | Sample_data_processing | Data analysis was performed using the BRB-ARRAYTOOLS software package (available at http://linus.nci.nih.gov/BRB-ArrayTools.html)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kay,,Klapproth
| | Sample_contact_email | kay.klapproth@uni-ulm.de
| | Sample_contact_phone | +49 (0) 731 50033834
| | Sample_contact_fax | +49 (0) 731 5002 28924
| | Sample_contact_department | Institute of Physiological Chemistry
| | Sample_contact_institute | Ulm University
| | Sample_contact_address | Albert-Einstein-Allee 11
| | Sample_contact_city | Ulm
| | Sample_contact_zip/postal_code | 89081
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM428nnn/GSM428957/suppl/GSM428957.CEL.gz
| | Sample_series_id | GSE17129
| | Sample_data_row_count | 54675
| | |
|
GSM428958 | GPL570 |
|
Ramos pRTS-GFP D+
|
BL Ramos cell line
|
transfection: pRTS-GFP
cell type: human Burkitt lymphoma
dsmz no.: ACC 603
origin: established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described to be EBV-negative but EBV infectible, to carry the (8;14) translocation and p53 mutations
|
NA
|
| Sample_geo_accession | GSM428958
| | Sample_status | Public on Jul 22 2009
| | Sample_submission_date | Jul 16 2009
| | Sample_last_update_date | Jul 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Ramos cells were transfected in triplicates with pRTS-GFP or pRTS-CA-IKK2, selected with hygromycin and transgene expression was induced with doxycycline (0,5mg/ml) for 48hrs. RNA was isolated with RNeasy mini kit (Qiagen, Venlo, Netherlands)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | extraction with RNeasy Mini Kit (QIAGEN) according to manufacturer´s instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2 µg of total RNA were labeled using the GeneChip® One-Cycle Target Labeling assay kit (Affymetrix), according to the manufacturer´s instructions
| | Sample_hyb_protocol | Hybridization on a Hybridization Oven 640 (Affymetrix).
| | Sample_hyb_protocol | Arrays were stained and washed in a FS 450 Fluidics station (Affymetrix)
| | Sample_scan_protocol | Imaging on an Affymetrix GeneChip (3000) scanner. Raw data were generated using the GCOS 1.4 software (Affymetrix). Probe level data were obtained using the Robust Multichip Average (RMA) normalization algorithm [Bolstad, B.M.,
| | Sample_scan_protocol | Irizarry, R.A., Astrand, M. & Speed, T.P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185?193 (2003)]
| | Sample_data_processing | Data pre-processed with RMA.
| | Sample_data_processing | Data analysis was performed using the BRB-ARRAYTOOLS software package (available at http://linus.nci.nih.gov/BRB-ArrayTools.html)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kay,,Klapproth
| | Sample_contact_email | kay.klapproth@uni-ulm.de
| | Sample_contact_phone | +49 (0) 731 50033834
| | Sample_contact_fax | +49 (0) 731 5002 28924
| | Sample_contact_department | Institute of Physiological Chemistry
| | Sample_contact_institute | Ulm University
| | Sample_contact_address | Albert-Einstein-Allee 11
| | Sample_contact_city | Ulm
| | Sample_contact_zip/postal_code | 89081
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM428nnn/GSM428958/suppl/GSM428958.CEL.gz
| | Sample_series_id | GSE17129
| | Sample_data_row_count | 54675
| | |
|
GSM428959 | GPL570 |
|
Ramos pRTS-CA-IKK2 E+
|
BL Ramos cell line
|
transfection: pRTS-CA-IKK2
cell type: human Burkitt lymphoma
dsmz no.: ACC 603
origin: established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described to be EBV-negative but EBV infectible, to carry the (8;14) translocation and p53 mutations
|
NA
|
| Sample_geo_accession | GSM428959
| | Sample_status | Public on Jul 22 2009
| | Sample_submission_date | Jul 16 2009
| | Sample_last_update_date | Jul 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Ramos cells were transfected in triplicates with pRTS-GFP or pRTS-CA-IKK2, selected with hygromycin and transgene expression was induced with doxycycline (0,5mg/ml) for 48hrs. RNA was isolated with RNeasy mini kit (Qiagen, Venlo, Netherlands)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | extraction with RNeasy Mini Kit (QIAGEN) according to manufacturer´s instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2 µg of total RNA were labeled using the GeneChip® One-Cycle Target Labeling assay kit (Affymetrix), according to the manufacturer´s instructions
| | Sample_hyb_protocol | Hybridization on a Hybridization Oven 640 (Affymetrix).
| | Sample_hyb_protocol | Arrays were stained and washed in a FS 450 Fluidics station (Affymetrix)
| | Sample_scan_protocol | Imaging on an Affymetrix GeneChip (3000) scanner. Raw data were generated using the GCOS 1.4 software (Affymetrix). Probe level data were obtained using the Robust Multichip Average (RMA) normalization algorithm [Bolstad, B.M.,
| | Sample_scan_protocol | Irizarry, R.A., Astrand, M. & Speed, T.P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185?193 (2003)]
| | Sample_data_processing | Data pre-processed with RMA.
| | Sample_data_processing | Data analysis was performed using the BRB-ARRAYTOOLS software package (available at http://linus.nci.nih.gov/BRB-ArrayTools.html)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kay,,Klapproth
| | Sample_contact_email | kay.klapproth@uni-ulm.de
| | Sample_contact_phone | +49 (0) 731 50033834
| | Sample_contact_fax | +49 (0) 731 5002 28924
| | Sample_contact_department | Institute of Physiological Chemistry
| | Sample_contact_institute | Ulm University
| | Sample_contact_address | Albert-Einstein-Allee 11
| | Sample_contact_city | Ulm
| | Sample_contact_zip/postal_code | 89081
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM428nnn/GSM428959/suppl/GSM428959.CEL.gz
| | Sample_series_id | GSE17129
| | Sample_data_row_count | 54675
| | |
|
GSM428960 | GPL570 |
|
Ramos pRTS-CA-IKK2 F+
|
BL Ramos cell line
|
transfection: pRTS-CA-IKK2
cell type: human Burkitt lymphoma
dsmz no.: ACC 603
origin: established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described to be EBV-negative but EBV infectible, to carry the (8;14) translocation and p53 mutations
|
NA
|
| Sample_geo_accession | GSM428960
| | Sample_status | Public on Jul 22 2009
| | Sample_submission_date | Jul 16 2009
| | Sample_last_update_date | Jul 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Ramos cells were transfected in triplicates with pRTS-GFP or pRTS-CA-IKK2, selected with hygromycin and transgene expression was induced with doxycycline (0,5mg/ml) for 48hrs. RNA was isolated with RNeasy mini kit (Qiagen, Venlo, Netherlands)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | extraction with RNeasy Mini Kit (QIAGEN) according to manufacturer´s instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2 µg of total RNA were labeled using the GeneChip® One-Cycle Target Labeling assay kit (Affymetrix), according to the manufacturer´s instructions
| | Sample_hyb_protocol | Hybridization on a Hybridization Oven 640 (Affymetrix).
| | Sample_hyb_protocol | Arrays were stained and washed in a FS 450 Fluidics station (Affymetrix)
| | Sample_scan_protocol | Imaging on an Affymetrix GeneChip (3000) scanner. Raw data were generated using the GCOS 1.4 software (Affymetrix). Probe level data were obtained using the Robust Multichip Average (RMA) normalization algorithm [Bolstad, B.M.,
| | Sample_scan_protocol | Irizarry, R.A., Astrand, M. & Speed, T.P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185?193 (2003)]
| | Sample_data_processing | Data pre-processed with RMA.
| | Sample_data_processing | Data analysis was performed using the BRB-ARRAYTOOLS software package (available at http://linus.nci.nih.gov/BRB-ArrayTools.html)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kay,,Klapproth
| | Sample_contact_email | kay.klapproth@uni-ulm.de
| | Sample_contact_phone | +49 (0) 731 50033834
| | Sample_contact_fax | +49 (0) 731 5002 28924
| | Sample_contact_department | Institute of Physiological Chemistry
| | Sample_contact_institute | Ulm University
| | Sample_contact_address | Albert-Einstein-Allee 11
| | Sample_contact_city | Ulm
| | Sample_contact_zip/postal_code | 89081
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM428nnn/GSM428960/suppl/GSM428960.CEL.gz
| | Sample_series_id | GSE17129
| | Sample_data_row_count | 54675
| | |
|
GSM428961 | GPL570 |
|
Ramos pRTS-CA-IKK2 H+
|
BL Ramos cell line
|
transfection: pRTS-CA-IKK2
cell type: human Burkitt lymphoma
dsmz no.: ACC 603
origin: established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described to be EBV-negative but EBV infectible, to carry the (8;14) translocation and p53 mutations
|
NA
|
| Sample_geo_accession | GSM428961
| | Sample_status | Public on Jul 22 2009
| | Sample_submission_date | Jul 16 2009
| | Sample_last_update_date | Jul 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Ramos cells were transfected in triplicates with pRTS-GFP or pRTS-CA-IKK2, selected with hygromycin and transgene expression was induced with doxycycline (0,5mg/ml) for 48hrs. RNA was isolated with RNeasy mini kit (Qiagen, Venlo, Netherlands)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | extraction with RNeasy Mini Kit (QIAGEN) according to manufacturer´s instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2 µg of total RNA were labeled using the GeneChip® One-Cycle Target Labeling assay kit (Affymetrix), according to the manufacturer´s instructions
| | Sample_hyb_protocol | Hybridization on a Hybridization Oven 640 (Affymetrix).
| | Sample_hyb_protocol | Arrays were stained and washed in a FS 450 Fluidics station (Affymetrix)
| | Sample_scan_protocol | Imaging on an Affymetrix GeneChip (3000) scanner. Raw data were generated using the GCOS 1.4 software (Affymetrix). Probe level data were obtained using the Robust Multichip Average (RMA) normalization algorithm [Bolstad, B.M.,
| | Sample_scan_protocol | Irizarry, R.A., Astrand, M. & Speed, T.P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185?193 (2003)]
| | Sample_data_processing | Data pre-processed with RMA.
| | Sample_data_processing | Data analysis was performed using the BRB-ARRAYTOOLS software package (available at http://linus.nci.nih.gov/BRB-ArrayTools.html)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kay,,Klapproth
| | Sample_contact_email | kay.klapproth@uni-ulm.de
| | Sample_contact_phone | +49 (0) 731 50033834
| | Sample_contact_fax | +49 (0) 731 5002 28924
| | Sample_contact_department | Institute of Physiological Chemistry
| | Sample_contact_institute | Ulm University
| | Sample_contact_address | Albert-Einstein-Allee 11
| | Sample_contact_city | Ulm
| | Sample_contact_zip/postal_code | 89081
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM428nnn/GSM428961/suppl/GSM428961.CEL.gz
| | Sample_series_id | GSE17129
| | Sample_data_row_count | 54675
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