Result

Search results for the GEO ID: GSE17140

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM429008

GPL339

myeloma cells control1

multiple myeloma cells (bone marrow)

agent: control disease state: multiple myeloma cell type: malignant plasma cells

myeloma cells control1

Sample_geo_accession	GSM429008
Sample_status	Public on Feb 28 2010
Sample_submission_date	Jul 16 2009
Sample_last_update_date	Dec 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	myeloma cells were cultured for 12h in serum free medium without IGF-1
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol (Gibco BRL, Carlsbad, CA), followed by a cleanup procedure with RNeasy columns (Qiagen, Cologne, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). Five microgram of total RNA was used to prepare biotinylated cRNA with the Enzo RNA transcript labeling kit (distributed by Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.2. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94 degrees C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
Sample_hyb_protocol	Fragmented cRNA was hybridised to mouse 430A arrays (Affymetrix) during 16h at 45 degrees C and 60rpm.
Sample_scan_protocol	The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
Sample_data_processing	Image files were analysed within GCOS (Affymetrix) and processed using the MAS5 algorithm.
Sample_platform_id	GPL339
Sample_contact_name	Lieven,,Thorrez
Sample_contact_institute	Katholieke Universiteit Leuven
Sample_contact_address	Kasteelpark Arenberg 10
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429008/suppl/GSM429008.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429008/suppl/GSM429008.CHP.gz
Sample_series_id	GSE17140
Sample_data_row_count	22690

GSM429009

GPL339

myeloma cells control2

multiple myeloma cells (bone marrow)

agent: control disease state: multiple myeloma cell type: malignant plasma cells

myeloma cells control2

Sample_geo_accession	GSM429009
Sample_status	Public on Feb 28 2010
Sample_submission_date	Jul 16 2009
Sample_last_update_date	Dec 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	myeloma cells were cultured for 12h in serum free medium without IGF-1
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol (Gibco BRL, Carlsbad, CA), followed by a cleanup procedure with RNeasy columns (Qiagen, Cologne, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). Five microgram of total RNA was used to prepare biotinylated cRNA with the Enzo RNA transcript labeling kit (distributed by Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.2. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94 degrees C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
Sample_hyb_protocol	Fragmented cRNA was hybridised to mouse 430A arrays (Affymetrix) during 16h at 45 degrees C and 60rpm.
Sample_scan_protocol	The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
Sample_data_processing	Image files were analysed within GCOS (Affymetrix) and processed using the MAS5 algorithm.
Sample_platform_id	GPL339
Sample_contact_name	Lieven,,Thorrez
Sample_contact_institute	Katholieke Universiteit Leuven
Sample_contact_address	Kasteelpark Arenberg 10
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429009/suppl/GSM429009.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429009/suppl/GSM429009.CHP.gz
Sample_series_id	GSE17140
Sample_data_row_count	22690

GSM429010

GPL339

myeloma cells control3

multiple myeloma cells (bone marrow)

agent: control disease state: multiple myeloma cell type: malignant plasma cells

myeloma cells control3

Sample_geo_accession	GSM429010
Sample_status	Public on Feb 28 2010
Sample_submission_date	Jul 16 2009
Sample_last_update_date	Dec 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	myeloma cells were cultured for 12h in serum free medium without IGF-1
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol (Gibco BRL, Carlsbad, CA), followed by a cleanup procedure with RNeasy columns (Qiagen, Cologne, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). Five microgram of total RNA was used to prepare biotinylated cRNA with the Enzo RNA transcript labeling kit (distributed by Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.2. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94 degrees C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
Sample_hyb_protocol	Fragmented cRNA was hybridised to mouse 430A arrays (Affymetrix) during 16h at 45 degrees C and 60rpm.
Sample_scan_protocol	The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
Sample_data_processing	Image files were analysed within GCOS (Affymetrix) and processed using the MAS5 algorithm.
Sample_platform_id	GPL339
Sample_contact_name	Lieven,,Thorrez
Sample_contact_institute	Katholieke Universiteit Leuven
Sample_contact_address	Kasteelpark Arenberg 10
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429010/suppl/GSM429010.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429010/suppl/GSM429010.CHP.gz
Sample_series_id	GSE17140
Sample_data_row_count	22690

GSM429011

GPL339

myeloma cells_IGF1_1

multiple myeloma cells (bone marrow)

agent: IGF-1 disease state: multiple myeloma cell type: malignant plasma cells

myeloma cells IGF1 treated 1

Sample_geo_accession	GSM429011
Sample_status	Public on Feb 28 2010
Sample_submission_date	Jul 16 2009
Sample_last_update_date	Dec 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	myeloma cells were treated with IGF-1 (100 ng/ml) for 12h in serum free medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol (Gibco BRL, Carlsbad, CA), followed by a cleanup procedure with RNeasy columns (Qiagen, Cologne, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). Five microgram of total RNA was used to prepare biotinylated cRNA with the Enzo RNA transcript labeling kit (distributed by Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.2. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94 degrees C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
Sample_hyb_protocol	Fragmented cRNA was hybridised to mouse 430A arrays (Affymetrix) during 16h at 45 degrees C and 60rpm.
Sample_scan_protocol	The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
Sample_data_processing	Image files were analysed within GCOS (Affymetrix) and processed using the MAS5 algorithm.
Sample_platform_id	GPL339
Sample_contact_name	Lieven,,Thorrez
Sample_contact_institute	Katholieke Universiteit Leuven
Sample_contact_address	Kasteelpark Arenberg 10
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429011/suppl/GSM429011.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429011/suppl/GSM429011.CHP.gz
Sample_series_id	GSE17140
Sample_data_row_count	22690

GSM429012

GPL339

myeloma cells_IGF1_2

multiple myeloma cells (bone marrow)

agent: IGF-1 disease state: multiple myeloma cell type: malignant plasma cells

myeloma cells IGF-1 treated 2

Sample_geo_accession	GSM429012
Sample_status	Public on Feb 28 2010
Sample_submission_date	Jul 16 2009
Sample_last_update_date	Dec 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	myeloma cells were treated with IGF-1 (100 ng/ml) for 12h in serum free medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol (Gibco BRL, Carlsbad, CA), followed by a cleanup procedure with RNeasy columns (Qiagen, Cologne, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). Five microgram of total RNA was used to prepare biotinylated cRNA with the Enzo RNA transcript labeling kit (distributed by Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.2. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94 degrees C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
Sample_hyb_protocol	Fragmented cRNA was hybridised to mouse 430A arrays (Affymetrix) during 16h at 45 degrees C and 60rpm.
Sample_scan_protocol	The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
Sample_data_processing	Image files were analysed within GCOS (Affymetrix) and processed using the MAS5 algorithm.
Sample_platform_id	GPL339
Sample_contact_name	Lieven,,Thorrez
Sample_contact_institute	Katholieke Universiteit Leuven
Sample_contact_address	Kasteelpark Arenberg 10
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429012/suppl/GSM429012.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429012/suppl/GSM429012.CHP.gz
Sample_series_id	GSE17140
Sample_data_row_count	22690

GSM429013

GPL339

myeloma cells_IGF1_3

multiple myeloma cells (bone marrow)

agent: IGF-1 disease state: multiple myeloma cell type: malignant plasma cells

myeloma cells IGF-1 treated 3

Sample_geo_accession	GSM429013
Sample_status	Public on Feb 28 2010
Sample_submission_date	Jul 16 2009
Sample_last_update_date	Dec 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	myeloma cells were treated with IGF-1 (100 ng/ml) for 12h in serum free medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol (Gibco BRL, Carlsbad, CA), followed by a cleanup procedure with RNeasy columns (Qiagen, Cologne, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). Five microgram of total RNA was used to prepare biotinylated cRNA with the Enzo RNA transcript labeling kit (distributed by Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.2. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94 degrees C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
Sample_hyb_protocol	Fragmented cRNA was hybridised to mouse 430A arrays (Affymetrix) during 16h at 45 degrees C and 60rpm.
Sample_scan_protocol	The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
Sample_data_processing	Image files were analysed within GCOS (Affymetrix) and processed using the MAS5 algorithm.
Sample_platform_id	GPL339
Sample_contact_name	Lieven,,Thorrez
Sample_contact_institute	Katholieke Universiteit Leuven
Sample_contact_address	Kasteelpark Arenberg 10
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429013/suppl/GSM429013.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM429nnn/GSM429013/suppl/GSM429013.CHP.gz
Sample_series_id	GSE17140
Sample_data_row_count	22690

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