Result

Search results for the GEO ID: GSE17263

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GSM ID

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Title

Source name

Description

Characteristics

GSM432386

GPL1261

Control, replicate 1

mouse uterus, wild-type SmoM2

mouse type: SmoM2 (control) tissue: uterus

Control, replicate 1

Sample_geo_accession	GSM432386
Sample_status	Public on Apr 13 2010
Sample_submission_date	Jul 22 2009
Sample_last_update_date	Apr 13 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals. SmoM2-YFP mice were obtained from Dr. Andrew P. McMahon. We generated mice with constitutive activation of Smo in the uterus by crossing SmoM2 mice with the PR-cre mouse model. These mice have Smo activated in all PR-positive cells which includes all compartments of the uterus. Because the SmoM2 allele was fused to YFP, we could use detection of YFP to validate activation of the SmoM2 allele. Immunofluorescence for YFP demonstrated that Smo was activated in the stroma of SmoM2 uteri but not in control uteri.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Mouse430_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM432nnn/GSM432386/suppl/GSM432386.cel.gz
Sample_series_id	GSE17263
Sample_data_row_count	45101

GSM432387

GPL1261

Control, replicate 2

mouse uterus, wild-type SmoM2

mouse type: SmoM2 (control) tissue: uterus

Control, replicate 2

Sample_geo_accession	GSM432387
Sample_status	Public on Apr 13 2010
Sample_submission_date	Jul 22 2009
Sample_last_update_date	Apr 13 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals. SmoM2-YFP mice were obtained from Dr. Andrew P. McMahon. We generated mice with constitutive activation of Smo in the uterus by crossing SmoM2 mice with the PR-cre mouse model. These mice have Smo activated in all PR-positive cells which includes all compartments of the uterus. Because the SmoM2 allele was fused to YFP, we could use detection of YFP to validate activation of the SmoM2 allele. Immunofluorescence for YFP demonstrated that Smo was activated in the stroma of SmoM2 uteri but not in control uteri.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Mouse430_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM432nnn/GSM432387/suppl/GSM432387.cel.gz
Sample_series_id	GSE17263
Sample_data_row_count	45101

GSM432388

GPL1261

Control, replicate 3

mouse uterus, wild-type SmoM2

mouse type: SmoM2 (control) tissue: uterus

Control, replicate 3

Sample_geo_accession	GSM432388
Sample_status	Public on Apr 13 2010
Sample_submission_date	Jul 22 2009
Sample_last_update_date	Apr 13 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals. SmoM2-YFP mice were obtained from Dr. Andrew P. McMahon. We generated mice with constitutive activation of Smo in the uterus by crossing SmoM2 mice with the PR-cre mouse model. These mice have Smo activated in all PR-positive cells which includes all compartments of the uterus. Because the SmoM2 allele was fused to YFP, we could use detection of YFP to validate activation of the SmoM2 allele. Immunofluorescence for YFP demonstrated that Smo was activated in the stroma of SmoM2 uteri but not in control uteri.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Mouse430_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM432nnn/GSM432388/suppl/GSM432388.cel.gz
Sample_series_id	GSE17263
Sample_data_row_count	45101

GSM432389

GPL1261

SmoM2, replicate 1

mouse uterus, mutant SmoM2

mouse type: Mutant SmoM2 tissue: uterus

SmoM2, replicate 1

Sample_geo_accession	GSM432389
Sample_status	Public on Apr 13 2010
Sample_submission_date	Jul 22 2009
Sample_last_update_date	Apr 13 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals. SmoM2-YFP mice were obtained from Dr. Andrew P. McMahon. We generated mice with constitutive activation of Smo in the uterus by crossing SmoM2 mice with the PR-cre mouse model. These mice have Smo activated in all PR-positive cells which includes all compartments of the uterus. Because the SmoM2 allele was fused to YFP, we could use detection of YFP to validate activation of the SmoM2 allele. Immunofluorescence for YFP demonstrated that Smo was activated in the stroma of SmoM2 uteri but not in control uteri.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Mouse430_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM432nnn/GSM432389/suppl/GSM432389.cel.gz
Sample_series_id	GSE17263
Sample_data_row_count	45101

GSM432390

GPL1261

SmoM2, replicate 2

mouse uterus, mutant SmoM2

mouse type: Mutant SmoM2 tissue: uterus

SmoM2, replicate 2

Sample_geo_accession	GSM432390
Sample_status	Public on Apr 13 2010
Sample_submission_date	Jul 22 2009
Sample_last_update_date	Apr 13 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals. SmoM2-YFP mice were obtained from Dr. Andrew P. McMahon. We generated mice with constitutive activation of Smo in the uterus by crossing SmoM2 mice with the PR-cre mouse model. These mice have Smo activated in all PR-positive cells which includes all compartments of the uterus. Because the SmoM2 allele was fused to YFP, we could use detection of YFP to validate activation of the SmoM2 allele. Immunofluorescence for YFP demonstrated that Smo was activated in the stroma of SmoM2 uteri but not in control uteri.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Mouse430_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM432nnn/GSM432390/suppl/GSM432390.cel.gz
Sample_series_id	GSE17263
Sample_data_row_count	45101

GSM432391

GPL1261

SmoM2, replicate 3

mouse uterus, mutant SmoM2

mouse type: Mutant SmoM2 tissue: uterus

SmoM2, replicate 3

Sample_geo_accession	GSM432391
Sample_status	Public on Apr 13 2010
Sample_submission_date	Jul 22 2009
Sample_last_update_date	Apr 13 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Mice were maintained in the designated animal care facility at Baylor College of Medicine according to the institutional guidelines for the care and use of laboratory animals. SmoM2-YFP mice were obtained from Dr. Andrew P. McMahon. We generated mice with constitutive activation of Smo in the uterus by crossing SmoM2 mice with the PR-cre mouse model. These mice have Smo activated in all PR-positive cells which includes all compartments of the uterus. Because the SmoM2 allele was fused to YFP, we could use detection of YFP to validate activation of the SmoM2 allele. Immunofluorescence for YFP demonstrated that Smo was activated in the stroma of SmoM2 uteri but not in control uteri.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized on Mouse430_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM432nnn/GSM432391/suppl/GSM432391.cel.gz
Sample_series_id	GSE17263
Sample_data_row_count	45101

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