Search results for the GEO ID: GSE17347  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM433761 | GPL570 |
|
A459 1-2D
|
A459, 2D
|
cell line: A459
cell type: lung carcinoma
genotype: hypotriploid
|
First RNA isolated from conventional 2D cell culture
|
| Sample_geo_accession | GSM433761
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433761/suppl/GSM433761.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433761/suppl/GSM433761.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433762 | GPL570 |
|
A459 2-2D
|
A459, 2D
|
cell line: A459
cell type: lung carcinoma
genotype: hypotriploid
|
Second RNA isolated from conventional 2D cell culture
|
| Sample_geo_accession | GSM433762
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433762/suppl/GSM433762.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433762/suppl/GSM433762.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433763 | GPL570 |
|
A459 3-2D
|
A459, 2D
|
cell line: A459
cell type: lung carcinoma
genotype: hypotriploid
|
Third RNA isolated from conventional 2D cell culture
|
| Sample_geo_accession | GSM433763
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433763/suppl/GSM433763.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433763/suppl/GSM433763.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433764 | GPL570 |
|
A459 1-3D
|
A459, 3D
|
cell line: A459
cell type: lung carcinoma
genotype: hypotriploid
|
First RNA isolated from 3D cell culture
|
| Sample_geo_accession | GSM433764
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433764/suppl/GSM433764.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433764/suppl/GSM433764.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433765 | GPL570 |
|
A459 2-3D
|
A459, 3D
|
cell line: A459
cell type: lung carcinoma
genotype: hypotriploid
|
Second RNA isolated from 3D cell culture
|
| Sample_geo_accession | GSM433765
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433765/suppl/GSM433765.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433765/suppl/GSM433765.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433766 | GPL570 |
|
A459 3-3D
|
A459, 3D
|
cell line: A459
cell type: lung carcinoma
genotype: hypotriploid
|
Third RNA isolated from 3D cell culture
|
| Sample_geo_accession | GSM433766
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433766/suppl/GSM433766.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433766/suppl/GSM433766.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433767 | GPL570 |
|
UT-15 1-2D
|
UT-15, 2D
|
cell line: UT-15
cell type: squamous cell carcinoma
genotype: p53 deficient
|
First RNA isolated from conventional 2D cell culture
|
| Sample_geo_accession | GSM433767
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433767/suppl/GSM433767.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433767/suppl/GSM433767.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433768 | GPL570 |
|
UT-15 2-2D
|
UT-15, 2D
|
cell line: UT-15
cell type: squamous cell carcinoma
genotype: p53 deficient
|
Second RNA isolated from conventional 2D cell culture
|
| Sample_geo_accession | GSM433768
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433768/suppl/GSM433768.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433768/suppl/GSM433768.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433769 | GPL570 |
|
UT-15 3-2D
|
UT-15, 2D
|
cell line: UT-15
cell type: squamous cell carcinoma
genotype: p53 deficient
|
Third RNA isolated from conventional 2D cell culture
|
| Sample_geo_accession | GSM433769
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433769/suppl/GSM433769.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433769/suppl/GSM433769.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433770 | GPL570 |
|
UT-15 1-3D
|
UT-15, 3D
|
cell line: UT-15
cell type: squamous cell carcinoma
genotype: p53 deficient
|
First RNA isolated from 3D cell culture
|
| Sample_geo_accession | GSM433770
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433770/suppl/GSM433770.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433770/suppl/GSM433770.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433771 | GPL570 |
|
UT-15 2-3D
|
UT-15, 3D
|
cell line: UT-15
cell type: squamous cell carcinoma
genotype: p53 deficient
|
Second RNA isolated from 3D cell culture
|
| Sample_geo_accession | GSM433771
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433771/suppl/GSM433771.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433771/suppl/GSM433771.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
| | |
|
GSM433772 | GPL570 |
|
UT-15 3-3D
|
UT-15, 3D
|
cell line: UT-15
cell type: squamous cell carcinoma
genotype: p53 deficient
|
Third RNA isolated from 3D cell culture
|
| Sample_geo_accession | GSM433772
| | Sample_status | Public on Jul 01 2010
| | Sample_submission_date | Jul 27 2009
| | Sample_last_update_date | Jul 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | RNA isolation of cells growing exponentially for 4 days in 2 dimensional cell culture or 3 dimensional cell culture (matrigel embedded cells)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Following cleanup according to Qiagen Rneasy Mini Kit Protocol.
| | Sample_label_ch1 | Biotinylated Nucleotides
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | The data were analyzed with Expression Console version 1.1 using RMA as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433772/suppl/GSM433772.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433772/suppl/GSM433772.chp.gz
| | Sample_series_id | GSE17347
| | Sample_data_row_count | 54675
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