Result

Search results for the GEO ID: GSE17348

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GSM ID

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Title

Source name

Description

Characteristics

GSM433774

GPL1261

Obl-MDA-PCa2b-CM

conditioned medium of MDA-PCa2b cells

cell type: primary osteoblast stage: day 10 of diffferentiation

conditioned medium of MDA-PCa2b cells

Sample_geo_accession	GSM433774
Sample_status	Public on Jul 01 2010
Sample_submission_date	Jul 27 2009
Sample_last_update_date	Aug 25 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After serum-starvation over night, cells were incubated for 6 hours with condtioned medium of MDA-PCa2b or PC-3 cells.
Sample_growth_protocol_ch1	Osteoblasts were grown in alpha-MEM with 10 % fetal calf serum. Cells were diffferentiated for 10 days in the presence of ascorbic acid (50 µg/ml) and ß-glycerophosphate (10 mM).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Rneasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software GCOS 1.4 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 300.
Sample_platform_id	GPL1261
Sample_contact_name	Thomas,,Streichert
Sample_contact_department	Institute For Clinical Chemistry / Central Laboratories
Sample_contact_institute	University Medical Center Hamburg-Eppendorf
Sample_contact_address	Martinistr. 52, Campus Forschung N27, 2. OG
Sample_contact_city	Hamburg
Sample_contact_zip/postal_code	D-20246
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433774/suppl/GSM433774.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433774/suppl/GSM433774.CHP.gz
Sample_series_id	GSE17348
Sample_data_row_count	45101

GSM433775

GPL1261

Obl-MDA-PC-3-CM

conditioned medium of PC-3 cells

cell type: primary osteoblast stage: day 10 of diffferentiation

conditioned medium of PC-3 cells

Sample_geo_accession	GSM433775
Sample_status	Public on Jul 01 2010
Sample_submission_date	Jul 27 2009
Sample_last_update_date	Aug 25 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After serum-starvation over night, cells were incubated for 6 hours with condtioned medium of MDA-PCa2b or PC-3 cells.
Sample_growth_protocol_ch1	Osteoblasts were grown in alpha-MEM with 10 % fetal calf serum. Cells were diffferentiated for 10 days in the presence of ascorbic acid (50 µg/ml) and ß-glycerophosphate (10 mM).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Rneasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software GCOS 1.4 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 300.
Sample_platform_id	GPL1261
Sample_contact_name	Thomas,,Streichert
Sample_contact_department	Institute For Clinical Chemistry / Central Laboratories
Sample_contact_institute	University Medical Center Hamburg-Eppendorf
Sample_contact_address	Martinistr. 52, Campus Forschung N27, 2. OG
Sample_contact_city	Hamburg
Sample_contact_zip/postal_code	D-20246
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433775/suppl/GSM433775.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM433nnn/GSM433775/suppl/GSM433775.CHP.gz
Sample_series_id	GSE17348
Sample_data_row_count	45101

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