Search results for the GEO ID: GSE17375  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM434140 | GPL570 |
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C100 TOP-GFP high rep 1
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C100 Colon CSC culture
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cell type: colon cancer stem cells (CSC)
expression: high Wnt
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10% highest TOP-GFP fraction sorted by FACS
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| Sample_geo_accession | GSM434140
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Jul 28 2009
| | Sample_last_update_date | Jul 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing = Expression data (cel files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Louis ,,Vermeulen
| | Sample_contact_email | l.vermeulen@amc.uva.nl
| | Sample_contact_phone | +31 20 5664777
| | Sample_contact_laboratory | LEXOR (Lab for Exp. Oncology and Radiobiology)
| | Sample_contact_department | Center for Experimental Molecular Medicine
| | Sample_contact_institute | Academic Medical Center Amsterdam
| | Sample_contact_address | Meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1105 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434140/suppl/GSM434140.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434140/suppl/GSM434140.CHP.gz
| | Sample_series_id | GSE17375
| | Sample_data_row_count | 54675
| | |
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GSM434141 | GPL570 |
|
C100 TOP-GFP low rep 1
|
C100 CSC culture
|
cell type: colon cancer stem cells (CSC)
expression: low Wnt
|
10% lowest TOP-GFP fraction sorted by FACS
|
| Sample_geo_accession | GSM434141
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Jul 28 2009
| | Sample_last_update_date | Jul 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing = Expression data (cel files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Louis ,,Vermeulen
| | Sample_contact_email | l.vermeulen@amc.uva.nl
| | Sample_contact_phone | +31 20 5664777
| | Sample_contact_laboratory | LEXOR (Lab for Exp. Oncology and Radiobiology)
| | Sample_contact_department | Center for Experimental Molecular Medicine
| | Sample_contact_institute | Academic Medical Center Amsterdam
| | Sample_contact_address | Meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1105 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434141/suppl/GSM434141.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434141/suppl/GSM434141.CHP.gz
| | Sample_series_id | GSE17375
| | Sample_data_row_count | 54675
| | |
|
GSM434142 | GPL570 |
|
C200 TOP-GFP high rep 2
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C200 CSC culture
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cell type: colon cancer stem cells (CSC)
expression: high Wnt
|
10% higest TOP-GFP fraction sorted by FACS
|
| Sample_geo_accession | GSM434142
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Jul 28 2009
| | Sample_last_update_date | Jul 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing = Expression data (cel files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Louis ,,Vermeulen
| | Sample_contact_email | l.vermeulen@amc.uva.nl
| | Sample_contact_phone | +31 20 5664777
| | Sample_contact_laboratory | LEXOR (Lab for Exp. Oncology and Radiobiology)
| | Sample_contact_department | Center for Experimental Molecular Medicine
| | Sample_contact_institute | Academic Medical Center Amsterdam
| | Sample_contact_address | Meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1105 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434142/suppl/GSM434142.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434142/suppl/GSM434142.CHP.gz
| | Sample_series_id | GSE17375
| | Sample_data_row_count | 54675
| | |
|
GSM434143 | GPL570 |
|
C200 TOP-GFP low rep 2
|
C200 CSC culture
|
cell type: colon cancer stem cells (CSC)
expression: low Wnt
|
10% lowest TOP-GFP fraction sorted by FACS
|
| Sample_geo_accession | GSM434143
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Jul 28 2009
| | Sample_last_update_date | Jul 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing = Expression data (cel files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Louis ,,Vermeulen
| | Sample_contact_email | l.vermeulen@amc.uva.nl
| | Sample_contact_phone | +31 20 5664777
| | Sample_contact_laboratory | LEXOR (Lab for Exp. Oncology and Radiobiology)
| | Sample_contact_department | Center for Experimental Molecular Medicine
| | Sample_contact_institute | Academic Medical Center Amsterdam
| | Sample_contact_address | Meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1105 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434143/suppl/GSM434143.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM434nnn/GSM434143/suppl/GSM434143.CHP.gz
| | Sample_series_id | GSE17375
| | Sample_data_row_count | 54675
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