Search results for the GEO ID: GSE17494  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM436142 | GPL1261 |
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N2A
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Neuro2A
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cell line: Neuro2A (N2A)
cell type: neuroblastoma
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neuroblastoma cell line Neuro2A
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| Sample_geo_accession | GSM436142
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Aug 04 2009
| | Sample_last_update_date | Jan 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Srandard culture condition for Neuro2A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and purified with the use of Qiashredder spin-columns (Qiagen) and an RNeasy Plus Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS3000 scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuichi,,Tsukada
| | Sample_contact_email | ytsukada@bioreg.kyushu-u.ac.jp
| | Sample_contact_phone | +81-092-642-6820
| | Sample_contact_fax | +81-092-642-6819
| | Sample_contact_department | Medical Institute of Bioregulation
| | Sample_contact_institute | Kyushu university
| | Sample_contact_address | 3-1-1 Maidashi, Higashi-ku
| | Sample_contact_city | Fukuoka
| | Sample_contact_state | Fukuoka
| | Sample_contact_zip/postal_code | 812-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436142/suppl/GSM436142.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436142/suppl/GSM436142.CHP.gz
| | Sample_series_id | GSE17494
| | Sample_data_row_count | 45101
| | |
|
GSM436143 | GPL1261 |
|
N2A empty vector
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Neuro2A transfected with empty vector
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cell line: Neuro2A (N2A)
cell type: neuroblastoma
|
neuroblastoma cell line Neuro2A transfected with empty vector
|
| Sample_geo_accession | GSM436143
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Aug 04 2009
| | Sample_last_update_date | Jan 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Srandard culture condition for Neuro2A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and purified with the use of Qiashredder spin-columns (Qiagen) and an RNeasy Plus Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS3000 scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuichi,,Tsukada
| | Sample_contact_email | ytsukada@bioreg.kyushu-u.ac.jp
| | Sample_contact_phone | +81-092-642-6820
| | Sample_contact_fax | +81-092-642-6819
| | Sample_contact_department | Medical Institute of Bioregulation
| | Sample_contact_institute | Kyushu university
| | Sample_contact_address | 3-1-1 Maidashi, Higashi-ku
| | Sample_contact_city | Fukuoka
| | Sample_contact_state | Fukuoka
| | Sample_contact_zip/postal_code | 812-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436143/suppl/GSM436143.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436143/suppl/GSM436143.CHP.gz
| | Sample_series_id | GSE17494
| | Sample_data_row_count | 45101
| | |
|
GSM436144 | GPL1261 |
|
N2A EGFP KD
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Neuro2A transfected with vector for EGFP knock down
|
cell line: Neuro2A (N2A)
cell type: neuroblastoma
|
neuroblastoma cell line Neuro2A transfected with vector for EGFP knock down
|
| Sample_geo_accession | GSM436144
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Aug 04 2009
| | Sample_last_update_date | Jan 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Srandard culture condition for Neuro2A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and purified with the use of Qiashredder spin-columns (Qiagen) and an RNeasy Plus Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS3000 scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuichi,,Tsukada
| | Sample_contact_email | ytsukada@bioreg.kyushu-u.ac.jp
| | Sample_contact_phone | +81-092-642-6820
| | Sample_contact_fax | +81-092-642-6819
| | Sample_contact_department | Medical Institute of Bioregulation
| | Sample_contact_institute | Kyushu university
| | Sample_contact_address | 3-1-1 Maidashi, Higashi-ku
| | Sample_contact_city | Fukuoka
| | Sample_contact_state | Fukuoka
| | Sample_contact_zip/postal_code | 812-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436144/suppl/GSM436144.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436144/suppl/GSM436144.CHP.gz
| | Sample_series_id | GSE17494
| | Sample_data_row_count | 45101
| | |
|
GSM436145 | GPL1261 |
|
N2A KDM7 KD1
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Neuro2A transfected with vector 1 for KDM7 knock down
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cell line: Neuro2A (N2A)
cell type: neuroblastoma
|
neuroblastoma cell line Neuro2A transfected with vector 1 for KDM7 knock down
|
| Sample_geo_accession | GSM436145
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Aug 04 2009
| | Sample_last_update_date | Jan 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Srandard culture condition for Neuro2A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and purified with the use of Qiashredder spin-columns (Qiagen) and an RNeasy Plus Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS3000 scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuichi,,Tsukada
| | Sample_contact_email | ytsukada@bioreg.kyushu-u.ac.jp
| | Sample_contact_phone | +81-092-642-6820
| | Sample_contact_fax | +81-092-642-6819
| | Sample_contact_department | Medical Institute of Bioregulation
| | Sample_contact_institute | Kyushu university
| | Sample_contact_address | 3-1-1 Maidashi, Higashi-ku
| | Sample_contact_city | Fukuoka
| | Sample_contact_state | Fukuoka
| | Sample_contact_zip/postal_code | 812-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436145/suppl/GSM436145.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436145/suppl/GSM436145.CHP.gz
| | Sample_series_id | GSE17494
| | Sample_data_row_count | 45101
| | |
|
GSM436146 | GPL1261 |
|
N2A KDM7 KD2
|
Neuro2A transfected with vector 2 for KDM7 knock down
|
cell line: Neuro2A (N2A)
cell type: neuroblastoma
|
neuroblastoma cell line Neuro2A transfected with vector 2 for KDM7 knock down
|
| Sample_geo_accession | GSM436146
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Aug 04 2009
| | Sample_last_update_date | Jan 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Srandard culture condition for Neuro2A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and purified with the use of Qiashredder spin-columns (Qiagen) and an RNeasy Plus Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS3000 scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuichi,,Tsukada
| | Sample_contact_email | ytsukada@bioreg.kyushu-u.ac.jp
| | Sample_contact_phone | +81-092-642-6820
| | Sample_contact_fax | +81-092-642-6819
| | Sample_contact_department | Medical Institute of Bioregulation
| | Sample_contact_institute | Kyushu university
| | Sample_contact_address | 3-1-1 Maidashi, Higashi-ku
| | Sample_contact_city | Fukuoka
| | Sample_contact_state | Fukuoka
| | Sample_contact_zip/postal_code | 812-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436146/suppl/GSM436146.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436146/suppl/GSM436146.CHP.gz
| | Sample_series_id | GSE17494
| | Sample_data_row_count | 45101
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