Search results for the GEO ID: GSE17508  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM436499 | GPL570 |
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MCF7-NC-rep1
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MCF7 cells after 24 hours of transfection with negative control RNA duplex
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tissue: breast cancer
cell line: MCF7
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expression pattern data from MCF7 cells after 24 hours of transfection with negative control RNA duplex
|
| Sample_geo_accession | GSM436499
| | Sample_status | Public on Dec 01 2012
| | Sample_submission_date | Aug 05 2009
| | Sample_last_update_date | Dec 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells were transfected with 50nm negative control RNA duplex or miR-22 duplex and total RNA was collected 24 hours after transfection.
| | Sample_growth_protocol_ch1 | MCF7 cells were maintained in DMEM supplemented with 10% fetal bovine serum, 100U/mL penicillin, and 100mg/mL streptomycin 37℃ with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating SoftwareVersion1.4 (GCOS1.4) using Affymetrix default analysis settings and global scaling as the normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jianhua,,Xiong
| | Sample_contact_email | xiong024@163.com
| | Sample_contact_phone | +86 10 62750799
| | Sample_contact_institute | Peking University
| | Sample_contact_address | Jinguang Life Science Building,Peking University,No.5 Yiheyuan Road
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100871
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436499/suppl/GSM436499.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436499/suppl/GSM436499.CHP.gz
| | Sample_series_id | GSE17508
| | Sample_data_row_count | 54675
| | |
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GSM436500 | GPL570 |
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MCF7-NC-rep2
|
MCF7 cells after 24 hours of transfection with negative control RNA duplex
|
tissue: breast cancer
cell line: MCF7
|
expression pattern data from MCF7 cells after 24 hours of transfection with negative control RNA duplex
|
| Sample_geo_accession | GSM436500
| | Sample_status | Public on Dec 01 2012
| | Sample_submission_date | Aug 05 2009
| | Sample_last_update_date | Dec 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells were transfected with 50nm negative control RNA duplex or miR-22 duplex and total RNA was collected 24 hours after transfection.
| | Sample_growth_protocol_ch1 | MCF7 cells were maintained in DMEM supplemented with 10% fetal bovine serum, 100U/mL penicillin, and 100mg/mL streptomycin 37℃ with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating SoftwareVersion1.4 (GCOS1.4) using Affymetrix default analysis settings and global scaling as the normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jianhua,,Xiong
| | Sample_contact_email | xiong024@163.com
| | Sample_contact_phone | +86 10 62750799
| | Sample_contact_institute | Peking University
| | Sample_contact_address | Jinguang Life Science Building,Peking University,No.5 Yiheyuan Road
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100871
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436500/suppl/GSM436500.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436500/suppl/GSM436500.CHP.gz
| | Sample_series_id | GSE17508
| | Sample_data_row_count | 54675
| | |
|
GSM436501 | GPL570 |
|
MCF7-NC-rep3
|
MCF7 cells after 24 hours of transfection with negative control RNA duplex
|
tissue: breast cancer
cell line: MCF7
|
expression pattern data from MCF7 cells after 24 hours of transfection with negative control RNA duplex
|
| Sample_geo_accession | GSM436501
| | Sample_status | Public on Dec 01 2012
| | Sample_submission_date | Aug 05 2009
| | Sample_last_update_date | Dec 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells were transfected with 50nm negative control RNA duplex or miR-22 duplex and total RNA was collected 24 hours after transfection.
| | Sample_growth_protocol_ch1 | MCF7 cells were maintained in DMEM supplemented with 10% fetal bovine serum, 100U/mL penicillin, and 100mg/mL streptomycin 37℃ with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating SoftwareVersion1.4 (GCOS1.4) using Affymetrix default analysis settings and global scaling as the normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jianhua,,Xiong
| | Sample_contact_email | xiong024@163.com
| | Sample_contact_phone | +86 10 62750799
| | Sample_contact_institute | Peking University
| | Sample_contact_address | Jinguang Life Science Building,Peking University,No.5 Yiheyuan Road
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100871
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436501/suppl/GSM436501.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436501/suppl/GSM436501.CHP.gz
| | Sample_series_id | GSE17508
| | Sample_data_row_count | 54675
| | |
|
GSM436502 | GPL570 |
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MCF7-miR-rep1
|
MCF7 cells after 24 hours of transfection with miR-22 duplex
|
tissue: breast cancer
cell line: MCF7
genome/variation: miR-22 duplex
|
expression pattern data from MCF7 cells after 24 hours of transfection with miR-22 duplex
|
| Sample_geo_accession | GSM436502
| | Sample_status | Public on Dec 01 2012
| | Sample_submission_date | Aug 05 2009
| | Sample_last_update_date | Dec 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells were transfected with 50nm negative control RNA duplex or miR-22 duplex and total RNA was collected 24 hours after transfection.
| | Sample_growth_protocol_ch1 | MCF7 cells were maintained in DMEM supplemented with 10% fetal bovine serum, 100U/mL penicillin, and 100mg/mL streptomycin 37℃ with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating SoftwareVersion1.4 (GCOS1.4) using Affymetrix default analysis settings and global scaling as the normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jianhua,,Xiong
| | Sample_contact_email | xiong024@163.com
| | Sample_contact_phone | +86 10 62750799
| | Sample_contact_institute | Peking University
| | Sample_contact_address | Jinguang Life Science Building,Peking University,No.5 Yiheyuan Road
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100871
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436502/suppl/GSM436502.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436502/suppl/GSM436502.CHP.gz
| | Sample_series_id | GSE17508
| | Sample_data_row_count | 54675
| | |
|
GSM436503 | GPL570 |
|
MCF7-miR-rep2
|
MCF7 cells after 24 hours of transfection with miR-22 duplex
|
tissue: breast cancer
cell line: MCF7
genome/variation: miR-22 duplex
|
expression pattern data from MCF7 cells after 24 hours of transfection with miR-22 duplex
|
| Sample_geo_accession | GSM436503
| | Sample_status | Public on Dec 01 2012
| | Sample_submission_date | Aug 05 2009
| | Sample_last_update_date | Dec 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells were transfected with 50nm negative control RNA duplex or miR-22 duplex and total RNA was collected 24 hours after transfection.
| | Sample_growth_protocol_ch1 | MCF7 cells were maintained in DMEM supplemented with 10% fetal bovine serum, 100U/mL penicillin, and 100mg/mL streptomycin 37℃ with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating SoftwareVersion1.4 (GCOS1.4) using Affymetrix default analysis settings and global scaling as the normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jianhua,,Xiong
| | Sample_contact_email | xiong024@163.com
| | Sample_contact_phone | +86 10 62750799
| | Sample_contact_institute | Peking University
| | Sample_contact_address | Jinguang Life Science Building,Peking University,No.5 Yiheyuan Road
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100871
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436503/suppl/GSM436503.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436503/suppl/GSM436503.CHP.gz
| | Sample_series_id | GSE17508
| | Sample_data_row_count | 54675
| | |
|
GSM436504 | GPL570 |
|
MCF7-miR-rep3
|
MCF7 cells after 24 hours of transfection with miR-22 duplex
|
tissue: breast cancer
cell line: MCF7
genome/variation: miR-22 duplex
|
expression pattern data from MCF7 cells after 24 hours of transfection with miR-22 duplex
|
| Sample_geo_accession | GSM436504
| | Sample_status | Public on Dec 01 2012
| | Sample_submission_date | Aug 05 2009
| | Sample_last_update_date | Dec 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells were transfected with 50nm negative control RNA duplex or miR-22 duplex and total RNA was collected 24 hours after transfection.
| | Sample_growth_protocol_ch1 | MCF7 cells were maintained in DMEM supplemented with 10% fetal bovine serum, 100U/mL penicillin, and 100mg/mL streptomycin 37℃ with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating SoftwareVersion1.4 (GCOS1.4) using Affymetrix default analysis settings and global scaling as the normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jianhua,,Xiong
| | Sample_contact_email | xiong024@163.com
| | Sample_contact_phone | +86 10 62750799
| | Sample_contact_institute | Peking University
| | Sample_contact_address | Jinguang Life Science Building,Peking University,No.5 Yiheyuan Road
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100871
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436504/suppl/GSM436504.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM436nnn/GSM436504/suppl/GSM436504.CHP.gz
| | Sample_series_id | GSE17508
| | Sample_data_row_count | 54675
| | |
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