Search results for the GEO ID: GSE17543  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM437400 | GPL570 |
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control siRNA transfected Human aortic vascular smooth muscle cells
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HAVSMC transfected control siRNA for 48hours
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tissue: aorta
cell type: vascular smooth muscle cells
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HAVSMC transfected control siRNA for 48hours
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| Sample_geo_accession | GSM437400
| | Sample_status | Public on Aug 07 2009
| | Sample_submission_date | Aug 06 2009
| | Sample_last_update_date | Aug 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human aortic vascular smooth muscle cells were transfected with 100nM of siRNA targeting human FoxM1 or non-targeting control using Invitrogen Lipofectamine 2000 reagent. After 48 hr culture, cells were harvested using RNAstat-60.
| | Sample_growth_protocol_ch1 | Human aortic vascular smooth muscle cells were cultured on 100mm plates in a single layer, and replated cell 24 hr before siRNA transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAstat-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug cRNA was hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipR Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | I-Ching,,Wang
| | Sample_contact_email | I-Ching.Wang@cchmc.org
| | Sample_contact_phone | 513-636-8230
| | Sample_contact_laboratory | Kalinichenko
| | Sample_contact_department | Pulmonary Biology
| | Sample_contact_institute | CCHMC
| | Sample_contact_address | 3333 Burnet Ave.
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45229
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM437nnn/GSM437400/suppl/GSM437400.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM437nnn/GSM437400/suppl/GSM437400.CHP.gz
| | Sample_series_id | GSE17543
| | Sample_data_row_count | 4584
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GSM437401 | GPL570 |
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FoxM1 siRNA transfected Human aortic vascular smooth muscle cells
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HAVSMC transfected FoxM1 siRNA for 48hours
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tissue: aorta
cell type: vascular smooth muscle cells
genome/variation: FoxM1 siRNA knock-down
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HAVSMC transfected FoxM1 siRNA for 48hours
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| Sample_geo_accession | GSM437401
| | Sample_status | Public on Aug 07 2009
| | Sample_submission_date | Aug 06 2009
| | Sample_last_update_date | Aug 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human aortic vascular smooth muscle cells were transfected with 100nM of siRNA targeting human FoxM1 or non-targeting control using Invitrogen Lipofectamine 2000 reagent. After 48 hr culture, cells were harvested using RNAstat-60.
| | Sample_growth_protocol_ch1 | Human aortic vascular smooth muscle cells were cultured on 100mm plates in a single layer, and replated cell 24 hr before siRNA transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAstat-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug cRNA was hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipR Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | I-Ching,,Wang
| | Sample_contact_email | I-Ching.Wang@cchmc.org
| | Sample_contact_phone | 513-636-8230
| | Sample_contact_laboratory | Kalinichenko
| | Sample_contact_department | Pulmonary Biology
| | Sample_contact_institute | CCHMC
| | Sample_contact_address | 3333 Burnet Ave.
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45229
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM437nnn/GSM437401/suppl/GSM437401.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM437nnn/GSM437401/suppl/GSM437401.CHP.gz
| | Sample_series_id | GSE17543
| | Sample_data_row_count | 4584
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