Search results for the GEO ID: GSE17592  |
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GSM ID | GPL ID |
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Title |
Source name |
Description |
Characteristics |
GSM438279 | GPL339 |
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E14tg2a-shDAB2-285-4
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E14tg2a cells were stably transfected with the plasmid encoding shDAB2-285; clone 4 of shDAB2-285 (285-4)
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cell type: Mouse embryonic stem cells
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Gene expression data from shDAB2-expressing E14tg2a
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| Sample_geo_accession | GSM438279
| | Sample_status | Public on Aug 12 2009
| | Sample_submission_date | Aug 11 2009
| | Sample_last_update_date | Aug 11 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In the normal growth condition as described above
| | Sample_growth_protocol_ch1 | The E14tg2a cells were cultured in GMEM supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, 1,000 U/ml LIF, 100 U/ml penicillin, and 100 mg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were isolated by Trizol (Invitrogen) and were further purified using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16-18 hr at 45C on Affymetrix Mouse 430A array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using 2500 Scanner.
| | Sample_data_processing | The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chien-Ling,,Huang
| | Sample_contact_email | m9105001@stmail.cgu.edu.tw
| | Sample_contact_institute | Chang Gung University
| | Sample_contact_address | 259 Wen-Hwa 1st Road, Kwei-Shan
| | Sample_contact_city | Taoyuan
| | Sample_contact_zip/postal_code | 333
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM438nnn/GSM438279/suppl/GSM438279.CEL.gz
| | Sample_series_id | GSE17592
| | Sample_data_row_count | 22626
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GSM438280 | GPL339 |
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E14tg2a-vector-9
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E14tg2a cells were stably transfected with the vector control plasmid; clone 9 of the vector control (ES-V9)
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cell type: Mouse embryonic stem cells
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Gene expression data from vector control E14tg2a
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| Sample_geo_accession | GSM438280
| | Sample_status | Public on Aug 12 2009
| | Sample_submission_date | Aug 11 2009
| | Sample_last_update_date | Aug 11 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In the normal growth condition as described above
| | Sample_growth_protocol_ch1 | The E14tg2a cells were cultured in GMEM supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, 1,000 U/ml LIF, 100 U/ml penicillin, and 100 mg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were isolated by Trizol (Invitrogen) and were further purified using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16-18 hr at 45C on Affymetrix Mouse 430A array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using 2500 Scanner.
| | Sample_data_processing | The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Chien-Ling,,Huang
| | Sample_contact_email | m9105001@stmail.cgu.edu.tw
| | Sample_contact_institute | Chang Gung University
| | Sample_contact_address | 259 Wen-Hwa 1st Road, Kwei-Shan
| | Sample_contact_city | Taoyuan
| | Sample_contact_zip/postal_code | 333
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM438nnn/GSM438280/suppl/GSM438280.CEL.gz
| | Sample_series_id | GSE17592
| | Sample_data_row_count | 22626
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