Search results for the GEO ID: GSE17639  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM440234 | GPL570 |
|
Adult Blood 15, biological replicate 1
|
Reticulocytes from adult periperal blood
|
tissue: peripheral blood
cell type: reticulocytes
developmental stage: adult
|
Gene expression data of reticulocytes from adult periperal blood.
|
| Sample_geo_accession | GSM440234
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440234/suppl/GSM440234.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440235 | GPL570 |
|
Adult Blood 16, biological replicate 2
|
Reticulocytes from adult periperal blood
|
tissue: peripheral blood
cell type: reticulocytes
developmental stage: adult
|
Gene expression data of reticulocytes from adult periperal blood.
|
| Sample_geo_accession | GSM440235
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440235/suppl/GSM440235.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440236 | GPL570 |
|
Adult Blood 17, biological replicate 3
|
Reticulocytes from adult periperal blood
|
tissue: peripheral blood
cell type: reticulocytes
developmental stage: adult
|
Gene expression data of reticulocytes from adult periperal blood.
|
| Sample_geo_accession | GSM440236
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440236/suppl/GSM440236.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440237 | GPL570 |
|
Adult Blood 18, biological replicate 4
|
Reticulocytes from adult periperal blood
|
tissue: peripheral blood
cell type: reticulocytes
developmental stage: adult
|
Gene expression data of reticulocytes from adult periperal blood.
|
| Sample_geo_accession | GSM440237
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440237/suppl/GSM440237.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440238 | GPL570 |
|
Adult Blood 19, biological replicate 5
|
Reticulocytes from adult periperal blood
|
tissue: peripheral blood
cell type: reticulocytes
developmental stage: adult
|
Gene expression data of reticulocytes from adult periperal blood.
|
| Sample_geo_accession | GSM440238
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440238/suppl/GSM440238.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440239 | GPL570 |
|
Adult Blood 20, biological replicate 6
|
Reticulocytes from adult periperal blood
|
tissue: peripheral blood
cell type: reticulocytes
developmental stage: adult
|
Gene expression data of reticulocytes from adult periperal blood.
|
| Sample_geo_accession | GSM440239
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440239/suppl/GSM440239.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440240 | GPL570 |
|
Cord Blood 15, biological replicate 1
|
Reticulocytes from umbilical cord blood
|
tissue: umbilical cord blood
cell type: reticulocytes
|
Gene expression data of reticulocytes from umbilical cord blood.
|
| Sample_geo_accession | GSM440240
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440240/suppl/GSM440240.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440241 | GPL570 |
|
Cord Blood 16, biological replicate 2
|
Reticulocytes from umbilical cord blood
|
tissue: umbilical cord blood
cell type: reticulocytes
|
Gene expression data of reticulocytes from umbilical cord blood.
|
| Sample_geo_accession | GSM440241
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440241/suppl/GSM440241.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440242 | GPL570 |
|
Cord Blood 17, biological replicate 3
|
Reticulocytes from umbilical cord blood
|
tissue: umbilical cord blood
cell type: reticulocytes
|
Gene expression data of reticulocytes from umbilical cord blood.
|
| Sample_geo_accession | GSM440242
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440242/suppl/GSM440242.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440243 | GPL570 |
|
Cord Blood 18, biological replicate 4
|
Reticulocytes from umbilical cord blood
|
tissue: umbilical cord blood
cell type: reticulocytes
|
Gene expression data of reticulocytes from umbilical cord blood.
|
| Sample_geo_accession | GSM440243
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440243/suppl/GSM440243.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
|
GSM440244 | GPL570 |
|
Cord Blood 19, biological replicate 5
|
Reticulocytes from umbilical cord blood
|
tissue: umbilical cord blood
cell type: reticulocytes
|
Gene expression data of reticulocytes from umbilical cord blood.
|
| Sample_geo_accession | GSM440244
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440244/suppl/GSM440244.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
| | |
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GSM440245 | GPL570 |
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Cord Blood 20, biological replicate 6
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Reticulocytes from umbilical cord blood
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tissue: umbilical cord blood
cell type: reticulocytes
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Gene expression data of reticulocytes from umbilical cord blood.
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| Sample_geo_accession | GSM440245
| | Sample_status | Public on Dec 01 2009
| | Sample_submission_date | Aug 13 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
| | Sample_growth_protocol_ch1 | NA
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM440nnn/GSM440245/suppl/GSM440245.CEL.gz
| | Sample_series_id | GSE17639
| | Sample_data_row_count | 54675
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