Search results for the GEO ID: GSE17760  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM443577 | GPL1261 |
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C57BL/6 neurospheres, biological rep1
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C57BL/6 7 day old neurosphere culture, generated from neural stem cells and progenitors isolated from adult lateral walls of the lateral ventricles
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gender: Female
strain: C57BL/6
sample type: neurospheres
culture age: 7 day
|
n/a
|
| Sample_geo_accession | GSM443577
| | Sample_status | Public on Jul 16 2010
| | Sample_submission_date | Aug 21 2009
| | Sample_last_update_date | Jul 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Neural stem cells and progenitors were isolated from the lateral walls of the lateral ventricles from 84 day year old Ts1Cje and littermate control C57BL/6 mice and cultured for 7 days in medium described previously in Merson et al., J Neurosci 2006; 26: 11359-70.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using a Qiagen Rneasy micro kit according to the manufacturer's instructions with a Dnase I digestion step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chelsee,A,Hewitt
| | Sample_contact_email | Chelsee.Hewitt@petermac.org
| | Sample_contact_phone | +61 3 9656 3524
| | Sample_contact_department | Pathology
| | Sample_contact_institute | The Peter MacCallum Cancer Centre
| | Sample_contact_address | St Andrews Place
| | Sample_contact_city | East Melbourne
| | Sample_contact_state | VIC
| | Sample_contact_zip/postal_code | 3051
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443577/suppl/GSM443577.CEL.gz
| | Sample_series_id | GSE17760
| | Sample_data_row_count | 45101
| | |
|
GSM443578 | GPL1261 |
|
Ts1Cje neurospheres, biological rep1
|
Ts1Cje 7 day old neurosphere culture, generated from neural stem cells and progenitors isolated from adult lateral walls of the lateral ventricles
|
gender: Female
strain: Ts1Cje on C57BL/6 background
sample type: neurospheres
culture age: 7 day
|
n/a
|
| Sample_geo_accession | GSM443578
| | Sample_status | Public on Jul 16 2010
| | Sample_submission_date | Aug 21 2009
| | Sample_last_update_date | Jul 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Neural stem cells and progenitors were isolated from the lateral walls of the lateral ventricles from 84 day year old Ts1Cje and littermate control C57BL/6 mice and cultured for 7 days in medium described previously in Merson et al., J Neurosci 2006; 26: 11359-70.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using a Qiagen Rneasy micro kit according to the manufacturer's instructions with a Dnase I digestion step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chelsee,A,Hewitt
| | Sample_contact_email | Chelsee.Hewitt@petermac.org
| | Sample_contact_phone | +61 3 9656 3524
| | Sample_contact_department | Pathology
| | Sample_contact_institute | The Peter MacCallum Cancer Centre
| | Sample_contact_address | St Andrews Place
| | Sample_contact_city | East Melbourne
| | Sample_contact_state | VIC
| | Sample_contact_zip/postal_code | 3051
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443578/suppl/GSM443578.CEL.gz
| | Sample_series_id | GSE17760
| | Sample_data_row_count | 45101
| | |
|
GSM443579 | GPL1261 |
|
C57BL/6 neurospheres, biological rep2
|
C57BL/6 7 day old neurosphere culture, generated from neural stem cells and progenitors isolated from adult lateral walls of the lateral ventricles
|
gender: Female
strain: C57BL/6
sample type: neurospheres
culture age: 7 day
|
n/a
|
| Sample_geo_accession | GSM443579
| | Sample_status | Public on Jul 16 2010
| | Sample_submission_date | Aug 21 2009
| | Sample_last_update_date | Jul 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Neural stem cells and progenitors were isolated from the lateral walls of the lateral ventricles from 84 day year old Ts1Cje and littermate control C57BL/6 mice and cultured for 7 days in medium described previously in Merson et al., J Neurosci 2006; 26: 11359-70.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using a Qiagen Rneasy micro kit according to the manufacturer's instructions with a Dnase I digestion step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chelsee,A,Hewitt
| | Sample_contact_email | Chelsee.Hewitt@petermac.org
| | Sample_contact_phone | +61 3 9656 3524
| | Sample_contact_department | Pathology
| | Sample_contact_institute | The Peter MacCallum Cancer Centre
| | Sample_contact_address | St Andrews Place
| | Sample_contact_city | East Melbourne
| | Sample_contact_state | VIC
| | Sample_contact_zip/postal_code | 3051
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443579/suppl/GSM443579.CEL.gz
| | Sample_series_id | GSE17760
| | Sample_data_row_count | 45101
| | |
|
GSM443580 | GPL1261 |
|
Ts1Cje neurospheres, biological rep2
|
Ts1Cje 7 day old neurosphere culture, generated from neural stem cells and progenitors isolated from adult lateral walls of the lateral ventricles
|
gender: Female
strain: Ts1Cje on C57BL/6 background
sample type: neurospheres
culture age: 7 day
|
n/a
|
| Sample_geo_accession | GSM443580
| | Sample_status | Public on Jul 16 2010
| | Sample_submission_date | Aug 21 2009
| | Sample_last_update_date | Jul 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Neural stem cells and progenitors were isolated from the lateral walls of the lateral ventricles from 84 day year old Ts1Cje and littermate control C57BL/6 mice and cultured for 7 days in medium described previously in Merson et al., J Neurosci 2006; 26: 11359-70.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using a Qiagen Rneasy micro kit according to the manufacturer's instructions with a Dnase I digestion step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chelsee,A,Hewitt
| | Sample_contact_email | Chelsee.Hewitt@petermac.org
| | Sample_contact_phone | +61 3 9656 3524
| | Sample_contact_department | Pathology
| | Sample_contact_institute | The Peter MacCallum Cancer Centre
| | Sample_contact_address | St Andrews Place
| | Sample_contact_city | East Melbourne
| | Sample_contact_state | VIC
| | Sample_contact_zip/postal_code | 3051
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443580/suppl/GSM443580.CEL.gz
| | Sample_series_id | GSE17760
| | Sample_data_row_count | 45101
| | |
|
GSM443581 | GPL1261 |
|
C57BL/6 neurospheres, biological rep3
|
C57BL/6 7 day old neurosphere culture, generated from neural stem cells and progenitors isolated from adult lateral walls of the lateral ventricles
|
gender: Female
strain: C57BL/6
sample type: neurospheres
culture age: 7 day
|
n/a
|
| Sample_geo_accession | GSM443581
| | Sample_status | Public on Jul 16 2010
| | Sample_submission_date | Aug 21 2009
| | Sample_last_update_date | Jul 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Neural stem cells and progenitors were isolated from the lateral walls of the lateral ventricles from 84 day year old Ts1Cje and littermate control C57BL/6 mice and cultured for 7 days in medium described previously in Merson et al., J Neurosci 2006; 26: 11359-70.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using a Qiagen Rneasy micro kit according to the manufacturer's instructions with a Dnase I digestion step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chelsee,A,Hewitt
| | Sample_contact_email | Chelsee.Hewitt@petermac.org
| | Sample_contact_phone | +61 3 9656 3524
| | Sample_contact_department | Pathology
| | Sample_contact_institute | The Peter MacCallum Cancer Centre
| | Sample_contact_address | St Andrews Place
| | Sample_contact_city | East Melbourne
| | Sample_contact_state | VIC
| | Sample_contact_zip/postal_code | 3051
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443581/suppl/GSM443581.CEL.gz
| | Sample_series_id | GSE17760
| | Sample_data_row_count | 45101
| | |
|
GSM443582 | GPL1261 |
|
Ts1Cje neurospheres, biological rep3
|
Ts1Cje 7 day old neurosphere culture, generated from neural stem cells and progenitors isolated from adult lateral walls of the lateral ventricles
|
gender: Female
strain: Ts1Cje on C57BL/6 background
sample type: neurospheres
culture age: 7 day
|
n/a
|
| Sample_geo_accession | GSM443582
| | Sample_status | Public on Jul 16 2010
| | Sample_submission_date | Aug 21 2009
| | Sample_last_update_date | Jul 12 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Neural stem cells and progenitors were isolated from the lateral walls of the lateral ventricles from 84 day year old Ts1Cje and littermate control C57BL/6 mice and cultured for 7 days in medium described previously in Merson et al., J Neurosci 2006; 26: 11359-70.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using a Qiagen Rneasy micro kit according to the manufacturer's instructions with a Dnase I digestion step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was processed according to the Australian Genome Research Facility (AGRF) protocol using the Two-Cycle Target cDNA synthesis kit (Millenium Sciences cat no. 900432), GeneChip Eukaryotic poly-A RNA spikes (Millenium Sciences cat no. 900433), Affymetrix GeneChip Sample CLeanup kit (Millenium Sciences cat no. 900371) and Affymetrix IVT labelling kit (Millenium Sciences cat no. 900449) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Labelled RNA samples were hybridised onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays according to the AGRFs protocol. Samples were prepared for hybridisation to the GeneChip by preparing a probe cocktail (cRNA @ 0.05ug/ul) that included 1x Hybridisation Buffer (100mM MES, 1m NaCl, 20mM EDTA, 0.01% Tween-20), 0.1mg/ml Herring Sperm DNA, 0.5mg/ml BSA, and 7% DMSO. A total hybridisation volume of 300ul was prepared for each sample and 200ul loaded into a GeneChip. The chip was hybridised at 45C for 16 hours in an oven with a rotating wheel at 60rpm. After hybridisation the chip was washed and stained with SAPE using the appropriate fluidics script in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Genechips were scanned using a GeneChip scanner 3000 with the scanner operating software, GCOS.
| | Sample_data_processing | Expression data were pre-processed and normalized using the gcRMA algorithm.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chelsee,A,Hewitt
| | Sample_contact_email | Chelsee.Hewitt@petermac.org
| | Sample_contact_phone | +61 3 9656 3524
| | Sample_contact_department | Pathology
| | Sample_contact_institute | The Peter MacCallum Cancer Centre
| | Sample_contact_address | St Andrews Place
| | Sample_contact_city | East Melbourne
| | Sample_contact_state | VIC
| | Sample_contact_zip/postal_code | 3051
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443582/suppl/GSM443582.CEL.gz
| | Sample_series_id | GSE17760
| | Sample_data_row_count | 45101
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