Search results for the GEO ID: GSE17777  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM443878 | GPL570 |
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Untreated control
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Pooled primary dermal human microvascular endothelial cells
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treatment: Untreated
cell type: primary dermal human microvascular endothelial cell
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n/a
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| Sample_geo_accession | GSM443878
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were mock treated (control) or treated for 4 hours with either VEGF (100pg/ml), VEGF + 8CPT (100 micromolar), or VEGF + ET [5 micrograms/ml protective antigen (PA) + 1 microgram/ml edema factor (EF)]
| | Sample_growth_protocol_ch1 | 10^6 HMVEC were starved overnight in EBM2 media supplemented with 0.1% BSA followed by treatment as indicated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the Qiagen Rneasy kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated RNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 10 ug of cRNA were hybridized for 16 hr at 45C on Affy U133A + 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,C,Doebele
| | Sample_contact_email | robert.doebele@ucdenver.edu
| | Sample_contact_phone | 3037242980
| | Sample_contact_fax | 3037243889
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 5841 S. Maryland Ave. MC 2115
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443878/suppl/GSM443878_No_Treat.CEL.gz
| | Sample_series_id | GSE17777
| | Sample_data_row_count | 54675
| | |
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GSM443879 | GPL570 |
|
VEGF
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Pooled primary dermal human microvascular endothelial cells
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treatment: VEGF
cell type: primary dermal human microvascular endothelial cell
|
n/a
|
| Sample_geo_accession | GSM443879
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were mock treated (control) or treated for 4 hours with either VEGF (100pg/ml), VEGF + 8CPT (100 micromolar), or VEGF + ET [5 micrograms/ml protective antigen (PA) + 1 microgram/ml edema factor (EF)]
| | Sample_growth_protocol_ch1 | 10^6 HMVEC were starved overnight in EBM2 media supplemented with 0.1% BSA followed by treatment as indicated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the Qiagen Rneasy kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated RNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 10 ug of cRNA were hybridized for 16 hr at 45C on Affy U133A + 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,C,Doebele
| | Sample_contact_email | robert.doebele@ucdenver.edu
| | Sample_contact_phone | 3037242980
| | Sample_contact_fax | 3037243889
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 5841 S. Maryland Ave. MC 2115
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443879/suppl/GSM443879_VEGF.CEL.gz
| | Sample_series_id | GSE17777
| | Sample_data_row_count | 54675
| | |
|
GSM443880 | GPL570 |
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VEGF + 8CPT
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Pooled primary dermal human microvascular endothelial cells
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treatment: VEGF + 8CPT
cell type: primary dermal human microvascular endothelial cell
|
n/a
|
| Sample_geo_accession | GSM443880
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were mock treated (control) or treated for 4 hours with either VEGF (100pg/ml), VEGF + 8CPT (100 micromolar), or VEGF + ET [5 micrograms/ml protective antigen (PA) + 1 microgram/ml edema factor (EF)]
| | Sample_growth_protocol_ch1 | 10^6 HMVEC were starved overnight in EBM2 media supplemented with 0.1% BSA followed by treatment as indicated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the Qiagen Rneasy kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated RNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 10 ug of cRNA were hybridized for 16 hr at 45C on Affy U133A + 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,C,Doebele
| | Sample_contact_email | robert.doebele@ucdenver.edu
| | Sample_contact_phone | 3037242980
| | Sample_contact_fax | 3037243889
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 5841 S. Maryland Ave. MC 2115
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443880/suppl/GSM443880_VEGF+EPAC.CEL.gz
| | Sample_series_id | GSE17777
| | Sample_data_row_count | 54675
| | |
|
GSM443881 | GPL570 |
|
VEGF + ET
|
Pooled primary dermal human microvascular endothelial cells
|
treatment: VEGF + ET
cell type: primary dermal human microvascular endothelial cell
|
n/a
|
| Sample_geo_accession | GSM443881
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were mock treated (control) or treated for 4 hours with either VEGF (100pg/ml), VEGF + 8CPT (100 micromolar), or VEGF + ET [5 micrograms/ml protective antigen (PA) + 1 microgram/ml edema factor (EF)]
| | Sample_growth_protocol_ch1 | 10^6 HMVEC were starved overnight in EBM2 media supplemented with 0.1% BSA followed by treatment as indicated
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the Qiagen Rneasy kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated RNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 10 ug of cRNA were hybridized for 16 hr at 45C on Affy U133A + 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,C,Doebele
| | Sample_contact_email | robert.doebele@ucdenver.edu
| | Sample_contact_phone | 3037242980
| | Sample_contact_fax | 3037243889
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 5841 S. Maryland Ave. MC 2115
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443881/suppl/GSM443881_VEGF+ET.CEL.gz
| | Sample_series_id | GSE17777
| | Sample_data_row_count | 54675
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