Search results for the GEO ID: GSE17782  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM443965 | GPL1352 |
|
Stem Cells Control 168
|
Stem Cells
|
tissue: adipose tissue
cell type: mesenchymal stem cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443965
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443965/suppl/GSM443965.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443965/suppl/GSM443965.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443966 | GPL1352 |
|
Stem Cells TGF-B 168
|
Stem Cells with TGF-B
|
tissue: adipose tissue
cell type: mesenchymal stem cells
treatment: With TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443966
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443966/suppl/GSM443966.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443966/suppl/GSM443966.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443967 | GPL1352 |
|
Stem Cells Control 207
|
Stem Cells
|
tissue: adipose tissue
cell type: mesenchymal stem cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443967
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443967/suppl/GSM443967.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443967/suppl/GSM443967.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443968 | GPL1352 |
|
Stem Cells TGF-B 207
|
Stem Cells with TGF-B
|
tissue: adipose tissue
cell type: mesenchymal stem cells
treatment: With TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443968
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443968/suppl/GSM443968.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443968/suppl/GSM443968.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443969 | GPL1352 |
|
Stem Cells Control 79
|
Stem Cells
|
tissue: adipose tissue
cell type: mesenchymal stem cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443969
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443969/suppl/GSM443969.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443969/suppl/GSM443969.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443970 | GPL1352 |
|
Stem CellsTGF-B 79
|
Stem Cells with TGF-B
|
tissue: adipose tissue
cell type: mesenchymal stem cells
treatment: With TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443970
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443970/suppl/GSM443970.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443970/suppl/GSM443970.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443971 | GPL1352 |
|
Disc Cells 18
|
Disc Cells
|
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443971
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443971/suppl/GSM443971.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443971/suppl/GSM443971.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443972 | GPL1352 |
|
Disc Cells 19
|
Disc Cells
|
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443972
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443972/suppl/GSM443972.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443972/suppl/GSM443972.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443973 | GPL1352 |
|
Disc Cells 22
|
Disc Cells
|
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443973
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443973/suppl/GSM443973.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443973/suppl/GSM443973.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443974 | GPL1352 |
|
Disc Cells 23
|
Disc Cells
|
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443974
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443974/suppl/GSM443974.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443974/suppl/GSM443974.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443975 | GPL1352 |
|
Disc Cells 24
|
Disc Cells
|
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443975
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443975/suppl/GSM443975.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443975/suppl/GSM443975.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443976 | GPL1352 |
|
Disc Cells 27
|
Disc Cells
|
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443976
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443976/suppl/GSM443976.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443976/suppl/GSM443976.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
GSM443977 | GPL1352 |
|
Disc Cells 30
|
Disc Cells
|
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
Grown in 3-D construct.
|
| Sample_geo_accession | GSM443977
| | Sample_status | Public on Aug 25 2009
| | Sample_submission_date | Aug 24 2009
| | Sample_last_update_date | Aug 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Stem cells were treated with or without 10ng/nl TGF-B3.
| | Sample_growth_protocol_ch1 | Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested according to instructions with the Trizol isolation method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
| | Sample_hyb_protocol | Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
| | Sample_scan_protocol | GeneChips were scanned once via the Affymetrix 3000G scanner.
| | Sample_data_processing | The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
| | Sample_platform_id | GPL1352
| | Sample_contact_name | Helen,,Gruber
| | Sample_contact_email | Helen.Gruber@carolinashealthcare.org
| | Sample_contact_laboratory | Orthopaedic Research
| | Sample_contact_department | Orthopaedic Surgery
| | Sample_contact_institute | Carolinas HealthCare System
| | Sample_contact_address | 1542 Graden Terrace
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443977/suppl/GSM443977.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM443nnn/GSM443977/suppl/GSM443977.CHP.gz
| | Sample_series_id | GSE17782
| | Sample_data_row_count | 61359
| | |
|
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