Search results for the GEO ID: GSE17948 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM448936 | GPL8300 |
|
mock-infected monocyte, biological rep1
|
Mock-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: none
|
Monocyte_MOCK-1
|
Sample_geo_accession | GSM448936
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448936/suppl/GSM448936.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448936/suppl/GSM448936.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448937 | GPL8300 |
|
mock-infected monocyte, biological rep2
|
Mock-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: none
|
Monocyte_MOCK-2
|
Sample_geo_accession | GSM448937
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448937/suppl/GSM448937.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448937/suppl/GSM448937.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448938 | GPL8300 |
|
mock-infected monocyte, biological rep3
|
Mock-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: none
|
Monocyte_MOCK-3
|
Sample_geo_accession | GSM448938
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448938/suppl/GSM448938.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448938/suppl/GSM448938.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448939 | GPL8300 |
|
mock-infected monocyte, biological rep4
|
Mock-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: none
|
Monocyte_MOCK-4
|
Sample_geo_accession | GSM448939
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448939/suppl/GSM448939.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448939/suppl/GSM448939.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448940 | GPL8300 |
|
HCMV-infected monocyte, biological rep1
|
HCMV-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: HCMV
|
Monocyte_HCMV-1
|
Sample_geo_accession | GSM448940
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448940/suppl/GSM448940.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448940/suppl/GSM448940.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448941 | GPL8300 |
|
HCMV-infected monocyte, biological rep2
|
HCMV-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: HCMV
|
Monocyte_HCMV-2
|
Sample_geo_accession | GSM448941
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448941/suppl/GSM448941.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448941/suppl/GSM448941.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448942 | GPL8300 |
|
HCMV-infected monocyte, biological rep3
|
HCMV-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: HCMV
|
Monocyte_HCMV-3
|
Sample_geo_accession | GSM448942
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448942/suppl/GSM448942.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448942/suppl/GSM448942.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448943 | GPL8300 |
|
HCMV-infected monocyte, biological rep4
|
HCMV-infected monocytes at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: none
infection: HCMV
|
Monocyte_HCMV-4
|
Sample_geo_accession | GSM448943
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448943/suppl/GSM448943.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448943/suppl/GSM448943.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448944 | GPL8300 |
|
HCMV-infected monocyte pretreated with anti-EGFR, biological rep1
|
HCMV-infected monocyte pretreated with anti-EGFR antibody at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: anti-EGFR antibody
infection: HCMV
|
Monocyte_HCMV_EGFR-1
|
Sample_geo_accession | GSM448944
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448944/suppl/GSM448944.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448944/suppl/GSM448944.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448945 | GPL8300 |
|
HCMV-infected monocyte pretreated with anti-EGFR, biological rep2
|
HCMV-infected monocyte pretreated with anti-EGFR antibody at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: anti-EGFR antibody
infection: HCMV
|
Monocyte_HCMV_EGFR-2
|
Sample_geo_accession | GSM448945
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448945/suppl/GSM448945.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448945/suppl/GSM448945.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448946 | GPL8300 |
|
HCMV-infected monocyte pretreated with anti-EGFR, biological rep3
|
HCMV-infected monocyte pretreated with anti-EGFR antibody at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: anti-EGFR antibody
infection: HCMV
|
Monocyte_HCMV_EGFR-3
|
Sample_geo_accession | GSM448946
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448946/suppl/GSM448946.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448946/suppl/GSM448946.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448947 | GPL8300 |
|
HCMV-infected monocyte pretreated with anti-EGFR, biological rep4
|
HCMV-infected monocyte pretreated with anti-EGFR antibody at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: anti-EGFR antibody
infection: HCMV
|
Monocyte_HCMV_EGFR-4
|
Sample_geo_accession | GSM448947
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448947/suppl/GSM448947.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448947/suppl/GSM448947.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448948 | GPL8300 |
|
HCMV-infected monocyte pretreated with AG, biological rep1
|
HCMV-infected monocyte pretreated with AG at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: AG
infection: HCMV
|
Monocyte_HCMV_AG-1
|
Sample_geo_accession | GSM448948
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448948/suppl/GSM448948.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448948/suppl/GSM448948.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448949 | GPL8300 |
|
HCMV-infected monocyte pretreated with AG, biological rep2
|
HCMV-infected monocyte pretreated with AG at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: AG
infection: HCMV
|
Monocyte_HCMV_AG-2
|
Sample_geo_accession | GSM448949
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448949/suppl/GSM448949.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448949/suppl/GSM448949.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448950 | GPL8300 |
|
HCMV-infected monocyte pretreated with AG, biological rep3
|
HCMV-infected monocyte pretreated with AG at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: AG
infection: HCMV
|
Monocyte_HCMV_AG-3
|
Sample_geo_accession | GSM448950
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448950/suppl/GSM448950.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448950/suppl/GSM448950.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
GSM448951 | GPL8300 |
|
HCMV-infected monocyte pretreated with AG, biological rep4
|
HCMV-infected monocyte pretreated with AG at 4 hours post infection
|
tissue: peripheral blood
cell type: monocytes
human cytomegalovirus strain: Towne/E
pretreatment: AG
infection: HCMV
|
Monocyte_HCMV_AG-4
|
Sample_geo_accession | GSM448951
| Sample_status | Public on Sep 03 2009
| Sample_submission_date | Sep 02 2009
| Sample_last_update_date | Sep 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 60 min with 10μg/ml of anti-EGFR antibody or 1 μM of the EGFR inhibitor AG1478 prior to infection. Cells were then HCMV infected (MOI 5) and incubated nonadherently at 37C for 24 hours.
| Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| Sample_platform_id | GPL8300
| Sample_contact_name | Andrew,D.,Yurochko
| Sample_contact_email | ayuroc@lsuhsc.edu
| Sample_contact_phone | 318-675-8332
| Sample_contact_fax | 318-675-5764
| Sample_contact_department | Microbiology & Immunology
| Sample_contact_institute | LSUHSC-S
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130-3932
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448951/suppl/GSM448951.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM448nnn/GSM448951/suppl/GSM448951.CHP.gz
| Sample_series_id | GSE17948
| Sample_data_row_count | 12625
| |
|
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