Result

Search results for the GEO ID: GSE17987

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM450325

GPL570

MIA.PACA2

human pancreatic tumour cell line, none

cell line: MiaPaca2 cell type: pancreatic tumor cells treatment: none

Expression analysis of human pancreatic tumor cells with no treatment.

Sample_geo_accession	GSM450325
Sample_status	Public on Sep 05 2009
Sample_submission_date	Sep 04 2009
Sample_last_update_date	Sep 04 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Masitinib (AB Science, Paris, France) was prepared from powder as a 10 or 20 mM stock solution in dimethyl sulfoxide and stored at −80°C. Gemcitabine (Gemzar, Lilly France) was obtained as a powder and dissolved in sterile 0.9% NaCl solution and stored as aliquots at -80°C. Fresh dilutions were prepared for each experiment.
Sample_growth_protocol_ch1	Cells were maintained in RPMI (BxPC-3, Capan-2) or DMEM (MiaPaca2, Panc1) medium containing glutamax-1 (Lonza), supplemented with 100 U/ml penicillin/100 µg/ml streptomycin, and 10% foetal calf serum (AbCys, Lot S02823S1800).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy mini kits. On column, DNase digestion was used.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix standard protocols.
Sample_hyb_protocol	Affymetrix standard protocols.
Sample_scan_protocol	Affymetrix standard protocols.
Sample_data_processing	RMA. Controls were removed, like all genes with low and poorly measured expression as defined by an expression value inferior to 100 units in all samples and an SD less than 0.1 on log2-transformed data. Thus, 8249 probe sets were selected and analysed.
Sample_platform_id	GPL570
Sample_contact_name	Pascal,,FINETTI
Sample_contact_email	finettip@ipc.unicancer.fnclcc.fr
Sample_contact_phone	(33)+4 91 22 33 04
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Centre de cancérologie de Marseille
Sample_contact_institute	Institut Paoli-Calmettes
Sample_contact_address	232 Bd Ste Marguerite
Sample_contact_city	Marseille
Sample_contact_state	BdR
Sample_contact_zip/postal_code	13009
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM450nnn/GSM450325/suppl/GSM450325.CEL.gz
Sample_series_id	GSE17987
Sample_data_row_count	8249

GSM450326

GPL570

MIA.PACA2 AB1010

human pancreatic tumour cell line, masitinib

cell line: MiaPaca2 cell type: pancreatic tumor cells treatment: masitinib

Expression analysis of human pancreatic tumor cells treated with masitinib only.

Sample_geo_accession	GSM450326
Sample_status	Public on Sep 05 2009
Sample_submission_date	Sep 04 2009
Sample_last_update_date	Sep 04 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Masitinib (AB Science, Paris, France) was prepared from powder as a 10 or 20 mM stock solution in dimethyl sulfoxide and stored at −80°C. Gemcitabine (Gemzar, Lilly France) was obtained as a powder and dissolved in sterile 0.9% NaCl solution and stored as aliquots at -80°C. Fresh dilutions were prepared for each experiment.
Sample_growth_protocol_ch1	Cells were maintained in RPMI (BxPC-3, Capan-2) or DMEM (MiaPaca2, Panc1) medium containing glutamax-1 (Lonza), supplemented with 100 U/ml penicillin/100 µg/ml streptomycin, and 10% foetal calf serum (AbCys, Lot S02823S1800).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy mini kits. On column, DNase digestion was used.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix standard protocols.
Sample_hyb_protocol	Affymetrix standard protocols.
Sample_scan_protocol	Affymetrix standard protocols.
Sample_data_processing	RMA. Controls were removed, like all genes with low and poorly measured expression as defined by an expression value inferior to 100 units in all samples and an SD less than 0.1 on log2-transformed data. Thus, 8249 probe sets were selected and analysed.
Sample_platform_id	GPL570
Sample_contact_name	Pascal,,FINETTI
Sample_contact_email	finettip@ipc.unicancer.fnclcc.fr
Sample_contact_phone	(33)+4 91 22 33 04
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Centre de cancérologie de Marseille
Sample_contact_institute	Institut Paoli-Calmettes
Sample_contact_address	232 Bd Ste Marguerite
Sample_contact_city	Marseille
Sample_contact_state	BdR
Sample_contact_zip/postal_code	13009
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM450nnn/GSM450326/suppl/GSM450326.CEL.gz
Sample_series_id	GSE17987
Sample_data_row_count	8249

GSM450327

GPL570

MIA.PACA2 GEM

human pancreatic tumour cell line, gemcitabine

cell line: MiaPaca2 cell type: pancreatic tumor cells treatment: gemcitabine

Expression analysis of human pancreatic tumor cells treated with gemcitabine only.

Sample_geo_accession	GSM450327
Sample_status	Public on Sep 05 2009
Sample_submission_date	Sep 04 2009
Sample_last_update_date	Sep 04 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Masitinib (AB Science, Paris, France) was prepared from powder as a 10 or 20 mM stock solution in dimethyl sulfoxide and stored at −80°C. Gemcitabine (Gemzar, Lilly France) was obtained as a powder and dissolved in sterile 0.9% NaCl solution and stored as aliquots at -80°C. Fresh dilutions were prepared for each experiment.
Sample_growth_protocol_ch1	Cells were maintained in RPMI (BxPC-3, Capan-2) or DMEM (MiaPaca2, Panc1) medium containing glutamax-1 (Lonza), supplemented with 100 U/ml penicillin/100 µg/ml streptomycin, and 10% foetal calf serum (AbCys, Lot S02823S1800).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy mini kits. On column, DNase digestion was used.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix standard protocols.
Sample_hyb_protocol	Affymetrix standard protocols.
Sample_scan_protocol	Affymetrix standard protocols.
Sample_data_processing	RMA. Controls were removed, like all genes with low and poorly measured expression as defined by an expression value inferior to 100 units in all samples and an SD less than 0.1 on log2-transformed data. Thus, 8249 probe sets were selected and analysed.
Sample_platform_id	GPL570
Sample_contact_name	Pascal,,FINETTI
Sample_contact_email	finettip@ipc.unicancer.fnclcc.fr
Sample_contact_phone	(33)+4 91 22 33 04
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Centre de cancérologie de Marseille
Sample_contact_institute	Institut Paoli-Calmettes
Sample_contact_address	232 Bd Ste Marguerite
Sample_contact_city	Marseille
Sample_contact_state	BdR
Sample_contact_zip/postal_code	13009
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM450nnn/GSM450327/suppl/GSM450327.CEL.gz
Sample_series_id	GSE17987
Sample_data_row_count	8249

GSM450328

GPL570

MIA.PACA2 both

human pancreatic tumour cell line, masitinib and gemcitabine

cell line: MiaPaca2 cell type: pancreatic tumor cells treatment: masitinib and gemcitabine

Expression analysis of human pancreatic tumor cells treated with both masitinib and gemcitabine.

Sample_geo_accession	GSM450328
Sample_status	Public on Sep 05 2009
Sample_submission_date	Sep 04 2009
Sample_last_update_date	Sep 04 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Masitinib (AB Science, Paris, France) was prepared from powder as a 10 or 20 mM stock solution in dimethyl sulfoxide and stored at −80°C. Gemcitabine (Gemzar, Lilly France) was obtained as a powder and dissolved in sterile 0.9% NaCl solution and stored as aliquots at -80°C. Fresh dilutions were prepared for each experiment.
Sample_growth_protocol_ch1	Cells were maintained in RPMI (BxPC-3, Capan-2) or DMEM (MiaPaca2, Panc1) medium containing glutamax-1 (Lonza), supplemented with 100 U/ml penicillin/100 µg/ml streptomycin, and 10% foetal calf serum (AbCys, Lot S02823S1800).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy mini kits. On column, DNase digestion was used.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix standard protocols.
Sample_hyb_protocol	Affymetrix standard protocols.
Sample_scan_protocol	Affymetrix standard protocols.
Sample_data_processing	RMA. Controls were removed, like all genes with low and poorly measured expression as defined by an expression value inferior to 100 units in all samples and an SD less than 0.1 on log2-transformed data. Thus, 8249 probe sets were selected and analysed.
Sample_platform_id	GPL570
Sample_contact_name	Pascal,,FINETTI
Sample_contact_email	finettip@ipc.unicancer.fnclcc.fr
Sample_contact_phone	(33)+4 91 22 33 04
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Centre de cancérologie de Marseille
Sample_contact_institute	Institut Paoli-Calmettes
Sample_contact_address	232 Bd Ste Marguerite
Sample_contact_city	Marseille
Sample_contact_state	BdR
Sample_contact_zip/postal_code	13009
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM450nnn/GSM450328/suppl/GSM450328.CEL.gz
Sample_series_id	GSE17987
Sample_data_row_count	8249

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