Search results for the GEO ID: GSE18043  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM250019 | GPL570 |
|
BM-MSC culture rep 1
|
bone marrow MSC in culture from normal donor 1
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 67 years
treatment: none
|
Gene expression data of human bone marrow derived mesenchymal stem cells.
BM-MSC of a male 67-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage.
|
| Sample_geo_accession | GSM250019
| | Sample_status | Public on Dec 15 2007
| | Sample_submission_date | Dec 14 2007
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250019/suppl/GSM250019.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250019/suppl/GSM250019.EXP.gz
| | Sample_relation | Reanalyzed by: GSE20125
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE9894
| | Sample_series_id | GSE18043
| | Sample_series_id | GSE24598
| | Sample_data_row_count | 54675
| | |
|
GSM250020 | GPL570 |
|
BM-MSC culture rep 2
|
bone marrow MSC in culture from normal donor 2
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 72 years
treatment: none
|
Gene expression data of human bone marrow derived mesenchymal stem cells.
BM-MSC of a male 72-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage.
|
| Sample_geo_accession | GSM250020
| | Sample_status | Public on Dec 15 2007
| | Sample_submission_date | Dec 14 2007
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250020/suppl/GSM250020.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250020/suppl/GSM250020.EXP.gz
| | Sample_relation | Reanalyzed by: GSE20125
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE9894
| | Sample_series_id | GSE18043
| | Sample_series_id | GSE24598
| | Sample_data_row_count | 54675
| | |
|
GSM250021 | GPL570 |
|
BM-MSC culture rep 3
|
bone marrow MSC in culture from normal donor 3
|
cell type: bone marrow-derived mesenchymal stem cells
gender: female
age: 74 years
treatment: none
|
Gene expression data of human bone marrow derived mesenchymal stem cells.
BM-MSC of a female 74-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage.
|
| Sample_geo_accession | GSM250021
| | Sample_status | Public on Dec 15 2007
| | Sample_submission_date | Dec 14 2007
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250021/suppl/GSM250021.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250021/suppl/GSM250021.EXP.gz
| | Sample_relation | Reanalyzed by: GSE20125
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE9894
| | Sample_series_id | GSE18043
| | Sample_series_id | GSE24598
| | Sample_data_row_count | 54675
| | |
|
GSM451153 | GPL570 |
|
BM-MSC culture osteogenic induction for 1 day rep 1
|
bone marrow MSC in culture, stimulated for osteogenic induction for 1 day from normal donor 1
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 67 years
treatment: dexamethasone
treatment time: 1 day
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 1 day.
BM-MSC of a male 67-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 1 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451153
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451153/suppl/GSM451153.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451153/suppl/GSM451153.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451154 | GPL570 |
|
BM-MSC culture osteogenic induction for 1 day rep 2
|
bone marrow MSC in culture, stimulated for osteogenic induction for 1 day from normal donor 2
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 72 years
treatment: dexamethasone
treatment time: 1 day
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 1 day.
BM-MSC of a male 72-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 1 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451154
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451154/suppl/GSM451154.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451154/suppl/GSM451154.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451155 | GPL570 |
|
BM-MSC culture osteogenic induction for 1 day rep 3
|
bone marrow MSC in culture, stimulated for osteogenic induction for 1 day from normal donor 3
|
cell type: bone marrow-derived mesenchymal stem cells
gender: female
age: 74 years
treatment: dexamethasone
treatment time: 1 day
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 1 day.
BM-MSC of a female 74-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 1 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451155
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451155/suppl/GSM451155.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451155/suppl/GSM451155.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451156 | GPL570 |
|
BM-MSC culture osteogenic induction for 3 days rep 1
|
bone marrow MSC in culture, stimulated for osteogenic induction for 3 days from normal donor 1
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 67 years
treatment: dexamethasone
treatment time: 3 days
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 3 days.
BM-MSC of a male 67-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 3 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451156
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451156/suppl/GSM451156.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451156/suppl/GSM451156.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451157 | GPL570 |
|
BM-MSC culture osteogenic induction for 3 days rep 2
|
bone marrow MSC in culture, stimulated for osteogenic induction for 3 days from normal donor 2
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 72 years
treatment: dexamethasone
treatment time: 3 days
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 3 days.
BM-MSC of a male 72-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 3 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451157
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451157/suppl/GSM451157.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451157/suppl/GSM451157.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451158 | GPL570 |
|
BM-MSC culture osteogenic induction for 3 days rep 3
|
bone marrow MSC in culture, stimulated for osteogenic induction for 3 days from normal donor 3
|
cell type: bone marrow-derived mesenchymal stem cells
gender: female
age: 74 years
treatment: dexamethasone
treatment time: 3 days
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 3 days.
BM-MSC of a female 74-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 3 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451158
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451158/suppl/GSM451158.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451158/suppl/GSM451158.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451159 | GPL570 |
|
BM-MSC culture osteogenic induction for 7 days rep 1
|
bone marrow MSC in culture, stimulated for osteogenic induction for 7 days from normal donor 1
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 67 years
treatment: dexamethasone
treatment time: 7 days
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 7 days.
BM-MSC of a male 67-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 7 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451159
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Sep 09 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451159/suppl/GSM451159.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451159/suppl/GSM451159.EXP.gz
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451160 | GPL570 |
|
BM-MSC culture osteogenic induction for 7 days rep 2
|
bone marrow MSC in culture, stimulated for osteogenic induction for 7 days from normal donor 2
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 72 years
treatment: dexamethasone
treatment time: 7 days
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 7 days.
BM-MSC of a male 72-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 7 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451160
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451160/suppl/GSM451160.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451160/suppl/GSM451160.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
GSM451161 | GPL570 |
|
BM-MSC culture osteogenic induction for 7 days rep 3
|
bone marrow MSC in culture, stimulated for osteogenic induction for 7 days from normal donor 3
|
cell type: bone marrow-derived mesenchymal stem cells
gender: female
age: 74 years
treatment: dexamethasone
treatment time: 7 days
|
Gene expression data of human bone marrow-derived mesenchymal stem cells after osteogenic induction for 7 days.
BM-MSC of a female 74-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage and induced with 10E-7 M dexamethasone for 7 day for osteogenic differentiation.
|
| Sample_geo_accession | GSM451161
| | Sample_status | Public on Sep 10 2009
| | Sample_submission_date | Sep 09 2009
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After expansion for 1 passage, cells were stimulated with 10E-7 M dexamethasone for osteogenic induction for 1, 3 and 7 days.
| | Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix).
| | Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database SiPaGene (www.bioretis.de).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Häupl
| | Sample_contact_email | thomas.haeupl@charite.de
| | Sample_contact_phone | +49(0)30 450513293
| | Sample_contact_fax | +49(0)30 450513957
| | Sample_contact_department | Rheumatology
| | Sample_contact_institute | Charité
| | Sample_contact_address | Tucholskystr. 2
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451161/suppl/GSM451161.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM451nnn/GSM451161/suppl/GSM451161.EXP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE18043
| | Sample_data_row_count | 54675
| | |
|
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(2 groups will be compared at a time) |
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