Search results for the GEO ID: GSE18113  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM452738 | GPL570 |
|
quiescent HMVECs rep1
|
Human microvascular endothelial cells (primary culture from Clonetics)
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL1A
|
| Sample_geo_accession | GSM452738
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452738/suppl/GSM452738.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452739 | GPL570 |
|
quiescent HMVECs rep2
|
Human microvascular endothelial cells (primary culture from Clonetics)
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL2A
|
| Sample_geo_accession | GSM452739
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452739/suppl/GSM452739.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452740 | GPL570 |
|
quiescent HMVECs rep3
|
Human microvascular endothelial cells (primary culture from Clonetics)
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL3A
|
| Sample_geo_accession | GSM452740
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452740/suppl/GSM452740.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452741 | GPL570 |
|
HMVECs co-cultured with LS180 cells rep1
|
Human microvascular endothelial cells (primary culture from Clonetics) + LS180 cells
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL1B
|
| Sample_geo_accession | GSM452741
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452741/suppl/GSM452741.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452742 | GPL570 |
|
HMVECs co-cultured with LS180 cells rep2
|
Human microvascular endothelial cells (primary culture from Clonetics) + LS180 cells
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL2B
|
| Sample_geo_accession | GSM452742
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452742/suppl/GSM452742.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452743 | GPL570 |
|
HMVECs co-cultured with LS180 cells rep3
|
Human microvascular endothelial cells (primary culture from Clonetics) + LS180 cells
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL3B
|
| Sample_geo_accession | GSM452743
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452743/suppl/GSM452743.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452744 | GPL570 |
|
HMVECs co-cultured with HT-29 cells rep1
|
Human microvascular endothelial cells (primary culture from Clonetics) + HT-29 cells
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL1C
|
| Sample_geo_accession | GSM452744
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452744/suppl/GSM452744.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452745 | GPL570 |
|
HMVECs co-cultured with HT-29 cells rep2
|
Human microvascular endothelial cells (primary culture from Clonetics) + HT-29 cells
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL2C
|
| Sample_geo_accession | GSM452745
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452745/suppl/GSM452745.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452746 | GPL570 |
|
HMVECs co-cultured with HT-29 cells rep3
|
Human microvascular endothelial cells (primary culture from Clonetics) + HT-29 cells
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL3C
|
| Sample_geo_accession | GSM452746
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452746/suppl/GSM452746.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452747 | GPL570 |
|
HMVECs co-cultured with blood components only rep1
|
Human microvascular endothelial cells (primary culture from Clonetics) + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL1alpha
|
| Sample_geo_accession | GSM452747
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452747/suppl/GSM452747.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452748 | GPL570 |
|
HMVECs co-cultured with blood components only rep2
|
Human microvascular endothelial cells (primary culture from Clonetics) + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL2alpha
|
| Sample_geo_accession | GSM452748
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452748/suppl/GSM452748.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452749 | GPL570 |
|
HMVECs co-cultured with blood components only rep3
|
Human microvascular endothelial cells (primary culture from Clonetics) + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL3alpha
|
| Sample_geo_accession | GSM452749
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452749/suppl/GSM452749.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452750 | GPL570 |
|
HMVECs co-cultured with LS180 cells and blood components rep1
|
Human microvascular endothelial cells (primary culture from Clonetics) + LS180 cells + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL1beta
|
| Sample_geo_accession | GSM452750
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452750/suppl/GSM452750.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452751 | GPL570 |
|
HMVECs co-cultured with LS180 cells and blood components rep2
|
Human microvascular endothelial cells (primary culture from Clonetics) + LS180 cells + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL2beta
|
| Sample_geo_accession | GSM452751
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452751/suppl/GSM452751.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452752 | GPL570 |
|
HMVECs co-cultured with LS180 cells and blood components rep3
|
Human microvascular endothelial cells (primary culture from Clonetics) + LS180 cells + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL3beta
|
| Sample_geo_accession | GSM452752
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452752/suppl/GSM452752.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452753 | GPL570 |
|
HMVECs co-cultured with HT-29 cells and blood components rep1
|
Human microvascular endothelial cells (primary culture from Clonetics) + HT-29 cells + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL1gamma
|
| Sample_geo_accession | GSM452753
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452753/suppl/GSM452753.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452754 | GPL570 |
|
HMVECs co-cultured with HT-29 cells and blood components rep2
|
Human microvascular endothelial cells (primary culture from Clonetics) + HT-29 cells + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL2gamma
|
| Sample_geo_accession | GSM452754
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452754/suppl/GSM452754.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
GSM452755 | GPL570 |
|
HMVECs co-cultured with HT-29 cells and blood components rep3
|
Human microvascular endothelial cells (primary culture from Clonetics) + HT-29 cells + blood components
|
tissue: endothelium
cell type: human microvascular endothelial cells (HMVECs)
|
HL3gamma
|
| Sample_geo_accession | GSM452755
| | Sample_status | Public on Oct 13 2009
| | Sample_submission_date | Sep 15 2009
| | Sample_last_update_date | Oct 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Tumor cells and/or blood components purified from healthy volunteers were added to HMVECs and co-cultured for 8 h. HMVECs were purified with the magnetic sorting to >95% purity.
| | Sample_growth_protocol_ch1 | HMVECs were grown to ~90% confluency according to Manufactures instroctions (Clonetics)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lubor,,Borsig
| | Sample_contact_email | lborsig@access.uzh.ch
| | Sample_contact_phone | +41 44 635 5134
| | Sample_contact_department | Institute of Physiology
| | Sample_contact_institute | University of Zürich
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zürich
| | Sample_contact_zip/postal_code | 8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM452nnn/GSM452755/suppl/GSM452755.CEL.gz
| | Sample_series_id | GSE18113
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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