Search results for the GEO ID: GSE18139  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM453594 | GPL570 |
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IMR-32
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mRNA from IMR-32
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treatment: untreated
cell line: IMR-32
cell type: neuroblastoma
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Untreated IMR-32 cells in regular growth medium.
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| Sample_geo_accession | GSM453594
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | Sep 16 2009
| | Sample_last_update_date | May 05 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Untreated.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 2mM L-glutamine, and Minimum Essential Medium supplemented with 10% FBS, 2mM L-glutamine, 1% non-essential amino acids and 1% sodium pyruvate, respectively.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | mRNA was isolated from the samples using the FastTrack 2.0 mRNA isolation kit according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 3 micrograms of mRNA per sample according to manufacturer's (Affymetrix) instructions.
| | Sample_hyb_protocol | Biotin-labelled target cRNAs were fragmented and the quality of labelling procedures was assessed with GeneChip Test3 arrays. Hybridizations to U133 Plus 2.0 arrays were performed for 16 hours at 45oC, followed by automated array washing and staining procedures.
| | Sample_scan_protocol | Arrays were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Basic GeneChip array analysis was performed for the obtained image file. Data preprocessing was done using the MAS5 algorithm implemented in the Bioconductor package “affy” (Gentleman et al., Genome Biol 2004, 5:R80).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maija,K,Wolf
| | Sample_contact_email | maija.wolf@helsinki.fi
| | Sample_contact_institute | Institute for Molecular Medicine Finland (FIMM)
| | Sample_contact_address | Biomedicum Helsinki 2U, Tukholmankatu 8,
| | Sample_contact_city | Helsinki
| | Sample_contact_zip/postal_code | FIN-00290
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453594/suppl/GSM453594.CEL.gz
| | Sample_series_id | GSE18139
| | Sample_series_id | GSE18144
| | Sample_data_row_count | 54675
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GSM453595 | GPL570 |
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NGP
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mRNA from NGP
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treatment: untreated
cell line: NGP
cell type: neuroblastoma
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Untreated NGP cells in regular growth medium.
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| Sample_geo_accession | GSM453595
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | Sep 16 2009
| | Sample_last_update_date | May 05 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Untreated.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 2mM L-glutamine, and Minimum Essential Medium supplemented with 10% FBS, 2mM L-glutamine, 1% non-essential amino acids and 1% sodium pyruvate, respectively.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | mRNA was isolated from the samples using the FastTrack 2.0 mRNA isolation kit according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 3 micrograms of mRNA per sample according to manufacturer's (Affymetrix) instructions.
| | Sample_hyb_protocol | Biotin-labelled target cRNAs were fragmented and the quality of labelling procedures was assessed with GeneChip Test3 arrays. Hybridizations to U133 Plus 2.0 arrays were performed for 16 hours at 45oC, followed by automated array washing and staining procedures.
| | Sample_scan_protocol | Arrays were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Basic GeneChip array analysis was performed for the obtained image file. Data preprocessing was done using the MAS5 algorithm implemented in the Bioconductor package “affy” (Gentleman et al., Genome Biol 2004, 5:R80).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maija,K,Wolf
| | Sample_contact_email | maija.wolf@helsinki.fi
| | Sample_contact_institute | Institute for Molecular Medicine Finland (FIMM)
| | Sample_contact_address | Biomedicum Helsinki 2U, Tukholmankatu 8,
| | Sample_contact_city | Helsinki
| | Sample_contact_zip/postal_code | FIN-00290
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453595/suppl/GSM453595.CEL.gz
| | Sample_series_id | GSE18139
| | Sample_series_id | GSE18144
| | Sample_data_row_count | 54675
| | |
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GSM453596 | GPL570 |
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Reference
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mRNA from a pooled sample consisting of 16 different cancer cell lines
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treatment: untreated
cell line: Pool of 16 different cancer cell lines
cell type: mixed
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Untreated reference cells in regular growth medium.
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| Sample_geo_accession | GSM453596
| | Sample_status | Public on May 05 2010
| | Sample_submission_date | Sep 16 2009
| | Sample_last_update_date | May 05 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Untreated.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 2mM L-glutamine, and Minimum Essential Medium supplemented with 10% FBS, 2mM L-glutamine, 1% non-essential amino acids and 1% sodium pyruvate, respectively.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | mRNA was isolated from the samples using the FastTrack 2.0 mRNA isolation kit according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 3 micrograms of mRNA per sample according to manufacturer's (Affymetrix) instructions.
| | Sample_hyb_protocol | Biotin-labelled target cRNAs were fragmented and the quality of labelling procedures was assessed with GeneChip Test3 arrays. Hybridizations to U133 Plus 2.0 arrays were performed for 16 hours at 45oC, followed by automated array washing and staining procedures.
| | Sample_scan_protocol | Arrays were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Basic GeneChip array analysis was performed for the obtained image file. Data preprocessing was done using the MAS5 algorithm implemented in the Bioconductor package “affy” (Gentleman et al., Genome Biol 2004, 5:R80).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maija,K,Wolf
| | Sample_contact_email | maija.wolf@helsinki.fi
| | Sample_contact_institute | Institute for Molecular Medicine Finland (FIMM)
| | Sample_contact_address | Biomedicum Helsinki 2U, Tukholmankatu 8,
| | Sample_contact_city | Helsinki
| | Sample_contact_zip/postal_code | FIN-00290
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453596/suppl/GSM453596.CEL.gz
| | Sample_series_id | GSE18139
| | Sample_series_id | GSE18144
| | Sample_data_row_count | 54675
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