Search results for the GEO ID: GSE18150  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM453728 | GPL570 |
|
Gliospheres_control_culture1
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Gliospheres, control, culture 1
|
treatment: control
culture: 1
cell type: glioblastoma multiforme cancer stem cells
|
none
|
| Sample_geo_accession | GSM453728
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Sep 17 2009
| | Sample_last_update_date | Sep 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Tumor samples of GBM from three patients undergoing surgical biopsies were obtained directly after surgical removal in accordance with local legislation (HUG protocol 04-113). Single cell suspensions, freshly isolated from tumor samples, were cultivated in DMEM-F12 (Gibco), supplemented with 20% BIT (Stem Cell Technologies) or B27 (1/50 Invitrogen), 10ng/ml recombinant human EGF (Invitrogen), 10ng/ml recombinant human basic FGF (Invitrogen) and 1% Penicillin/Streptomycin (Gibco). As previously described (4, 5, 7, 21), non-adherent cellular spheroids derived from these serum-free cultures conditions were considered as enriched cancer stem cells cultures and were used for subsequent experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each of the three treated primary cultures and their respective controls, using the RNeasy Mini Kit (QIAGEN, Valencia, CA) according to the manufacturer’s recommendations. The quality of total RNA was verified by an Agilent RNA 600 nanoassay and by measuring the 260/280 absorbance ratio.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Manufacturer's protocol
| | Sample_hyb_protocol | Manufacturer's protocol
| | Sample_scan_protocol | Manufacturer's protocol
| | Sample_data_processing | RMA, using the “affy” package from Bioconductor
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paolo,,Provero
| | Sample_contact_email | paolo.provero@gmail.com
| | Sample_contact_department | Genetics, Biology and Biochemistry
| | Sample_contact_institute | University of Turin
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_zip/postal_code | I-10100
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453728/suppl/GSM453728.CEL.gz
| | Sample_series_id | GSE18150
| | Sample_data_row_count | 54675
| | |
|
GSM453729 | GPL570 |
|
Gliospheres_DZNep_culture1
|
Gliospheres, DZNep treated, culture 1
|
treatment: treated
culture: 1
cell type: glioblastoma multiforme cancer stem cells
|
none
|
| Sample_geo_accession | GSM453729
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Sep 17 2009
| | Sample_last_update_date | Sep 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5uM DZNep was added to the cultures for 5 days
| | Sample_growth_protocol_ch1 | Tumor samples of GBM from three patients undergoing surgical biopsies were obtained directly after surgical removal in accordance with local legislation (HUG protocol 04-113). Single cell suspensions, freshly isolated from tumor samples, were cultivated in DMEM-F12 (Gibco), supplemented with 20% BIT (Stem Cell Technologies) or B27 (1/50 Invitrogen), 10ng/ml recombinant human EGF (Invitrogen), 10ng/ml recombinant human basic FGF (Invitrogen) and 1% Penicillin/Streptomycin (Gibco). As previously described (4, 5, 7, 21), non-adherent cellular spheroids derived from these serum-free cultures conditions were considered as enriched cancer stem cells cultures and were used for subsequent experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each of the three treated primary cultures and their respective controls, using the RNeasy Mini Kit (QIAGEN, Valencia, CA) according to the manufacturer’s recommendations. The quality of total RNA was verified by an Agilent RNA 600 nanoassay and by measuring the 260/280 absorbance ratio.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Manufacturer's protocol
| | Sample_hyb_protocol | Manufacturer's protocol
| | Sample_scan_protocol | Manufacturer's protocol
| | Sample_data_processing | RMA, using the “affy” package from Bioconductor
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paolo,,Provero
| | Sample_contact_email | paolo.provero@gmail.com
| | Sample_contact_department | Genetics, Biology and Biochemistry
| | Sample_contact_institute | University of Turin
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_zip/postal_code | I-10100
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453729/suppl/GSM453729.CEL.gz
| | Sample_series_id | GSE18150
| | Sample_data_row_count | 54675
| | |
|
GSM453730 | GPL570 |
|
Gliospheres_control_culture2
|
Gliospheres, control, culture 2
|
treatment: control
culture: 2
cell type: glioblastoma multiforme cancer stem cells
|
none
|
| Sample_geo_accession | GSM453730
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Sep 17 2009
| | Sample_last_update_date | Sep 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Tumor samples of GBM from three patients undergoing surgical biopsies were obtained directly after surgical removal in accordance with local legislation (HUG protocol 04-113). Single cell suspensions, freshly isolated from tumor samples, were cultivated in DMEM-F12 (Gibco), supplemented with 20% BIT (Stem Cell Technologies) or B27 (1/50 Invitrogen), 10ng/ml recombinant human EGF (Invitrogen), 10ng/ml recombinant human basic FGF (Invitrogen) and 1% Penicillin/Streptomycin (Gibco). As previously described (4, 5, 7, 21), non-adherent cellular spheroids derived from these serum-free cultures conditions were considered as enriched cancer stem cells cultures and were used for subsequent experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each of the three treated primary cultures and their respective controls, using the RNeasy Mini Kit (QIAGEN, Valencia, CA) according to the manufacturer’s recommendations. The quality of total RNA was verified by an Agilent RNA 600 nanoassay and by measuring the 260/280 absorbance ratio.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Manufacturer's protocol
| | Sample_hyb_protocol | Manufacturer's protocol
| | Sample_scan_protocol | Manufacturer's protocol
| | Sample_data_processing | RMA, using the “affy” package from Bioconductor
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paolo,,Provero
| | Sample_contact_email | paolo.provero@gmail.com
| | Sample_contact_department | Genetics, Biology and Biochemistry
| | Sample_contact_institute | University of Turin
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_zip/postal_code | I-10100
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453730/suppl/GSM453730.CEL.gz
| | Sample_series_id | GSE18150
| | Sample_data_row_count | 54675
| | |
|
GSM453731 | GPL570 |
|
Gliospheres_DZNep_culture2
|
Gliospheres, DZNep treated, culture 2
|
treatment: treated
culture: 2
cell type: glioblastoma multiforme cancer stem cells
|
none
|
| Sample_geo_accession | GSM453731
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Sep 17 2009
| | Sample_last_update_date | Sep 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5uM DZNep was added to the cultures for 5 days
| | Sample_growth_protocol_ch1 | Tumor samples of GBM from three patients undergoing surgical biopsies were obtained directly after surgical removal in accordance with local legislation (HUG protocol 04-113). Single cell suspensions, freshly isolated from tumor samples, were cultivated in DMEM-F12 (Gibco), supplemented with 20% BIT (Stem Cell Technologies) or B27 (1/50 Invitrogen), 10ng/ml recombinant human EGF (Invitrogen), 10ng/ml recombinant human basic FGF (Invitrogen) and 1% Penicillin/Streptomycin (Gibco). As previously described (4, 5, 7, 21), non-adherent cellular spheroids derived from these serum-free cultures conditions were considered as enriched cancer stem cells cultures and were used for subsequent experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each of the three treated primary cultures and their respective controls, using the RNeasy Mini Kit (QIAGEN, Valencia, CA) according to the manufacturer’s recommendations. The quality of total RNA was verified by an Agilent RNA 600 nanoassay and by measuring the 260/280 absorbance ratio.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Manufacturer's protocol
| | Sample_hyb_protocol | Manufacturer's protocol
| | Sample_scan_protocol | Manufacturer's protocol
| | Sample_data_processing | RMA, using the “affy” package from Bioconductor
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paolo,,Provero
| | Sample_contact_email | paolo.provero@gmail.com
| | Sample_contact_department | Genetics, Biology and Biochemistry
| | Sample_contact_institute | University of Turin
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_zip/postal_code | I-10100
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453731/suppl/GSM453731.CEL.gz
| | Sample_series_id | GSE18150
| | Sample_data_row_count | 54675
| | |
|
GSM453732 | GPL570 |
|
Gliospheres_control_culture3
|
Gliospheres, control, culture 3
|
treatment: control
culture: 3
cell type: glioblastoma multiforme cancer stem cells
|
none
|
| Sample_geo_accession | GSM453732
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Sep 17 2009
| | Sample_last_update_date | Sep 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Tumor samples of GBM from three patients undergoing surgical biopsies were obtained directly after surgical removal in accordance with local legislation (HUG protocol 04-113). Single cell suspensions, freshly isolated from tumor samples, were cultivated in DMEM-F12 (Gibco), supplemented with 20% BIT (Stem Cell Technologies) or B27 (1/50 Invitrogen), 10ng/ml recombinant human EGF (Invitrogen), 10ng/ml recombinant human basic FGF (Invitrogen) and 1% Penicillin/Streptomycin (Gibco). As previously described (4, 5, 7, 21), non-adherent cellular spheroids derived from these serum-free cultures conditions were considered as enriched cancer stem cells cultures and were used for subsequent experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each of the three treated primary cultures and their respective controls, using the RNeasy Mini Kit (QIAGEN, Valencia, CA) according to the manufacturer’s recommendations. The quality of total RNA was verified by an Agilent RNA 600 nanoassay and by measuring the 260/280 absorbance ratio.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Manufacturer's protocol
| | Sample_hyb_protocol | Manufacturer's protocol
| | Sample_scan_protocol | Manufacturer's protocol
| | Sample_data_processing | RMA, using the “affy” package from Bioconductor
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paolo,,Provero
| | Sample_contact_email | paolo.provero@gmail.com
| | Sample_contact_department | Genetics, Biology and Biochemistry
| | Sample_contact_institute | University of Turin
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_zip/postal_code | I-10100
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453732/suppl/GSM453732.CEL.gz
| | Sample_series_id | GSE18150
| | Sample_data_row_count | 54675
| | |
|
GSM453733 | GPL570 |
|
Gliospheres_DZNep_culture3
|
Gliospheres, DZNep treated, culture 3
|
treatment: treated
culture: 3
cell type: glioblastoma multiforme cancer stem cells
|
none
|
| Sample_geo_accession | GSM453733
| | Sample_status | Public on Nov 30 2009
| | Sample_submission_date | Sep 17 2009
| | Sample_last_update_date | Sep 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5uM DZNep was added to the cultures for 5 days
| | Sample_growth_protocol_ch1 | Tumor samples of GBM from three patients undergoing surgical biopsies were obtained directly after surgical removal in accordance with local legislation (HUG protocol 04-113). Single cell suspensions, freshly isolated from tumor samples, were cultivated in DMEM-F12 (Gibco), supplemented with 20% BIT (Stem Cell Technologies) or B27 (1/50 Invitrogen), 10ng/ml recombinant human EGF (Invitrogen), 10ng/ml recombinant human basic FGF (Invitrogen) and 1% Penicillin/Streptomycin (Gibco). As previously described (4, 5, 7, 21), non-adherent cellular spheroids derived from these serum-free cultures conditions were considered as enriched cancer stem cells cultures and were used for subsequent experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from each of the three treated primary cultures and their respective controls, using the RNeasy Mini Kit (QIAGEN, Valencia, CA) according to the manufacturer’s recommendations. The quality of total RNA was verified by an Agilent RNA 600 nanoassay and by measuring the 260/280 absorbance ratio.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Manufacturer's protocol
| | Sample_hyb_protocol | Manufacturer's protocol
| | Sample_scan_protocol | Manufacturer's protocol
| | Sample_data_processing | RMA, using the “affy” package from Bioconductor
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paolo,,Provero
| | Sample_contact_email | paolo.provero@gmail.com
| | Sample_contact_department | Genetics, Biology and Biochemistry
| | Sample_contact_institute | University of Turin
| | Sample_contact_address | Via Nizza 52
| | Sample_contact_city | Torino
| | Sample_contact_zip/postal_code | I-10100
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453733/suppl/GSM453733.CEL.gz
| | Sample_series_id | GSE18150
| | Sample_data_row_count | 54675
| | |
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