Search results for the GEO ID: GSE18161  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM453956 | GPL570 |
|
A1, 0x st_wash, 1st series
|
A1, 0x st_wash, 1st series
|
stringent wash cycles (total): 0x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 0x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453956
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453956/suppl/GSM453956_A1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453956/suppl/GSM453956_A1.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453957 | GPL570 |
|
A2, 2x st_wash, 1st series
|
A2, 2x st_wash, 1st series
|
stringent wash cycles (total): 2x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 2x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453957
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453957/suppl/GSM453957_A2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453957/suppl/GSM453957_A2.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453958 | GPL570 |
|
A3, 7x st_wash, 1st series
|
A3, 7x st_wash, 1st series
|
stringent wash cycles (total): 7x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 7x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453958
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453958/suppl/GSM453958_A3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453958/suppl/GSM453958_A3.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453959 | GPL570 |
|
A4, 17x st_wash, 1st series
|
A4, 17x st_wash, 1st series
|
stringent wash cycles (total): 17x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453959
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453959/suppl/GSM453959_A4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453959/suppl/GSM453959_A4.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453960 | GPL570 |
|
A5, 17x st_wash, 2nd series
|
A5, 17x st_wash, 2nd series
|
stringent wash cycles (total): 17x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453960
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453960/suppl/GSM453960_A5.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453960/suppl/GSM453960_A5.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453961 | GPL570 |
|
A6, 19x st_wash, 2nd series
|
A6, 19x st_wash, 2nd series
|
stringent wash cycles (total): 19x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 2x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453961
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453961/suppl/GSM453961_A6.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453961/suppl/GSM453961_A6.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453962 | GPL570 |
|
A7, 24x st_wash, 2nd series
|
A7, 24x st_wash, 2nd series
|
stringent wash cycles (total): 24x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 7x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453962
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453962/suppl/GSM453962_A7.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453962/suppl/GSM453962_A7.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453963 | GPL570 |
|
A8, 34x st_wash, 2nd series
|
A8, 34x st_wash, 2nd series
|
stringent wash cycles (total): 34x
initial stringent wash cycles before first staining (first series): 0x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 17x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453963
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453963/suppl/GSM453963_A8.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453963/suppl/GSM453963_A8.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453964 | GPL570 |
|
B1, 2x st_wash, 1st series
|
B1, 2x st_wash, 1st series
|
stringent wash cycles (total): 2x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 0x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453964
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453964/suppl/GSM453964_B1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453964/suppl/GSM453964_B1.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453965 | GPL570 |
|
B2, 4x st_wash, 1st series
|
B2, 4x st_wash, 1st series
|
stringent wash cycles (total): 4x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 2x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453965
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453965/suppl/GSM453965_B2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453965/suppl/GSM453965_B2.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453966 | GPL570 |
|
B3, 9x st_wash, 1st series
|
B3, 9x st_wash, 1st series
|
stringent wash cycles (total): 9x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 7x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453966
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453966/suppl/GSM453966_B3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453966/suppl/GSM453966_B3.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453967 | GPL570 |
|
B4, 19x st_wash, 1st series
|
B4, 19x st_wash, 1st series
|
stringent wash cycles (total): 19x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453967
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453967/suppl/GSM453967_B4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453967/suppl/GSM453967_B4.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453968 | GPL570 |
|
B5, 21x st_wash, 2nd series
|
B5, 21x st_wash, 2nd series
|
stringent wash cycles (total): 21x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 2x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453968
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453968/suppl/GSM453968_B5.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453968/suppl/GSM453968_B5.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453969 | GPL570 |
|
B6 23x st_wash, 2nd series
|
B6 23x st_wash, 2nd series
|
stringent wash cycles (total): 23x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 2x
additional stringent wash cycles after second staining (second series): 2x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453969
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453969/suppl/GSM453969_B6.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453969/suppl/GSM453969_B6.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453970 | GPL570 |
|
B7, 28x st_wash, 2nd series
|
B7, 28x st_wash, 2nd series
|
stringent wash cycles (total): 28x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 2x
additional stringent wash cycles after second staining (second series): 7x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453970
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453970/suppl/GSM453970_B7.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453970/suppl/GSM453970_B7.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453971 | GPL570 |
|
B8, 38x st_wash, 2nd series
|
B8, 38x st_wash, 2nd series
|
stringent wash cycles (total): 38x
initial stringent wash cycles before first staining (first series): 2x
additional stringent wash cycles after first staining (first series): 17x
initial stringent wash cycles before second staining (second series): 2x
additional stringent wash cycles after second staining (second series): 17x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453971
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453971/suppl/GSM453971_B8.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453971/suppl/GSM453971_B8.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453972 | GPL570 |
|
C1, 6x st_wash, 1st series
|
C1, 6x st_wash, 1st series
|
stringent wash cycles (total): 6x
initial stringent wash cycles before first staining (first series): 6x
additional stringent wash cycles after first staining (first series): 0x
initial stringent wash cycles before second staining (second series): 0x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): none
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453972
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453972/suppl/GSM453972_C1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453972/suppl/GSM453972_C1.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
| | |
|
GSM453973 | GPL570 |
|
C2, 12x st_wash, 2nd series
|
C2, 12x st_wash, 2nd series
|
stringent wash cycles (total): 12x
initial stringent wash cycles before first staining (first series): 6x
additional stringent wash cycles after first staining (first series): 0x
initial stringent wash cycles before second staining (second series): 6x
additional stringent wash cycles after second staining (second series): 0x
staining (first series): 3 rounds
staining (second series): 3 rounds
cell line: FTC133
|
identical RNA hybridised to three arrays (A, B, C), different stringent wash cycles
staining protocol: 3 rounds total composed of staining with streptavidin phycoerythrin (SAPE) in two rounds which are intermitted by a round of anti-SAPE antibody staining
|
| Sample_geo_accession | GSM453973
| | Sample_status | Public on Sep 22 2009
| | Sample_submission_date | Sep 18 2009
| | Sample_last_update_date | Sep 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were untreated.
| | Sample_growth_protocol_ch1 | FTC133 cells were cultured under standard conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by a column purification with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 25 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array U133 Plus 2.0. An Affymetrix Fluidics Station 400 was used for washing and staining.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChipScanner 3000 with the 7G upgrade.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Knut,,Krohn
| | Sample_contact_laboratory | DNA Technologies
| | Sample_contact_department | IZKF
| | Sample_contact_institute | University Leipzig
| | Sample_contact_address | Inselstraße 22
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453973/suppl/GSM453973_C2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453973/suppl/GSM453973_C2.mas5.CHP.gz
| | Sample_series_id | GSE18161
| | Sample_data_row_count | 54675
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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