Result

Search results for the GEO ID: GSE18162

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM453976

GPL1355

070314-control-48

placenta G20 control

tissue: placenta agent: saccharine solution alone (control)

Gene expression data from placenta of control dams

Sample_geo_accession	GSM453976
Sample_status	Public on Jan 29 2010
Sample_submission_date	Sep 18 2009
Sample_last_update_date	Sep 18 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On Gestational day 20, placentae were removed by cesarian section, saline perfused, frozen in liquid nitrogen and stored at -80C
Sample_growth_protocol_ch1	Dams were consuming 5%ethanol in 0.066% saccharine solution or saccharine solution alone (controls) during their pregnancy, averaging 16ml during a 4hr interval every day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performedwith RNeasy (Qiagen, Valencia,CA) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on affymetrix rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Daniel,,Savage
Sample_contact_email	dsavage@salud.unm.edu
Sample_contact_phone	505-272-8808
Sample_contact_fax	505-272-8082
Sample_contact_department	Neurosciences
Sample_contact_institute	University of New Mexico
Sample_contact_address	1University of New Mexico
Sample_contact_city	Albuquerque
Sample_contact_state	NM
Sample_contact_zip/postal_code	87131
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453976/suppl/GSM453976.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453976/suppl/GSM453976.CHP.gz
Sample_series_id	GSE18162
Sample_data_row_count	31099

GSM453977

GPL1355

070419-control-25

placenta G20 control

tissue: placenta agent: saccharine solution alone (control)

Gene expression data from placenta of control dams

Sample_geo_accession	GSM453977
Sample_status	Public on Jan 29 2010
Sample_submission_date	Sep 18 2009
Sample_last_update_date	Sep 18 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On Gestational day 20, placentae were removed by cesarian section, saline perfused, frozen in liquid nitrogen and stored at -80C
Sample_growth_protocol_ch1	Dams were consuming 5%ethanol in 0.066% saccharine solution or saccharine solution alone (controls) during their pregnancy, averaging 16ml during a 4hr interval every day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performedwith RNeasy (Qiagen, Valencia,CA) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on affymetrix rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Daniel,,Savage
Sample_contact_email	dsavage@salud.unm.edu
Sample_contact_phone	505-272-8808
Sample_contact_fax	505-272-8082
Sample_contact_department	Neurosciences
Sample_contact_institute	University of New Mexico
Sample_contact_address	1University of New Mexico
Sample_contact_city	Albuquerque
Sample_contact_state	NM
Sample_contact_zip/postal_code	87131
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453977/suppl/GSM453977.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453977/suppl/GSM453977.CHP.gz
Sample_series_id	GSE18162
Sample_data_row_count	31099

GSM453978

GPL1355

070419-control-37

placenta G20 control

tissue: placenta agent: saccharine solution alone (control)

Gene expression data from placenta of control dams

Sample_geo_accession	GSM453978
Sample_status	Public on Jan 29 2010
Sample_submission_date	Sep 18 2009
Sample_last_update_date	Sep 18 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On Gestational day 20, placentae were removed by cesarian section, saline perfused, frozen in liquid nitrogen and stored at -80C
Sample_growth_protocol_ch1	Dams were consuming 5%ethanol in 0.066% saccharine solution or saccharine solution alone (controls) during their pregnancy, averaging 16ml during a 4hr interval every day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performedwith RNeasy (Qiagen, Valencia,CA) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on affymetrix rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Daniel,,Savage
Sample_contact_email	dsavage@salud.unm.edu
Sample_contact_phone	505-272-8808
Sample_contact_fax	505-272-8082
Sample_contact_department	Neurosciences
Sample_contact_institute	University of New Mexico
Sample_contact_address	1University of New Mexico
Sample_contact_city	Albuquerque
Sample_contact_state	NM
Sample_contact_zip/postal_code	87131
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453978/suppl/GSM453978.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453978/suppl/GSM453978.CHP.gz
Sample_series_id	GSE18162
Sample_data_row_count	31099

GSM453979

GPL1355

070314_ETOH-20

placenta G20 ETOH

tissue: placenta agent: 5% ethanol in 0.066% saccharine solution

Gene expression data from placenta of ethanol consuming dams

Sample_geo_accession	GSM453979
Sample_status	Public on Jan 29 2010
Sample_submission_date	Sep 18 2009
Sample_last_update_date	Sep 18 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On Gestational day 20, placentae were removed by cesarian section, saline perfused, frozen in liquid nitrogen and stored at -80C
Sample_growth_protocol_ch1	Dams were consuming 5%ethanol in 0.066% saccharine solution or saccharine solution alone (controls) during their pregnancy, averaging 16ml during a 4hr interval every day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performedwith RNeasy (Qiagen, Valencia,CA) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on affymetrix rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Daniel,,Savage
Sample_contact_email	dsavage@salud.unm.edu
Sample_contact_phone	505-272-8808
Sample_contact_fax	505-272-8082
Sample_contact_department	Neurosciences
Sample_contact_institute	University of New Mexico
Sample_contact_address	1University of New Mexico
Sample_contact_city	Albuquerque
Sample_contact_state	NM
Sample_contact_zip/postal_code	87131
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453979/suppl/GSM453979.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453979/suppl/GSM453979.CHP.gz
Sample_series_id	GSE18162
Sample_data_row_count	31099

GSM453980

GPL1355

070314_ETOH-28

placenta G20 ETOH

tissue: placenta agent: 5% ethanol in 0.066% saccharine solution

Gene expression data from placenta of ethanol consuming dams

Sample_geo_accession	GSM453980
Sample_status	Public on Jan 29 2010
Sample_submission_date	Sep 18 2009
Sample_last_update_date	Sep 18 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On Gestational day 20, placentae were removed by cesarian section, saline perfused, frozen in liquid nitrogen and stored at -80C
Sample_growth_protocol_ch1	Dams were consuming 5%ethanol in 0.066% saccharine solution or saccharine solution alone (controls) during their pregnancy, averaging 16ml during a 4hr interval every day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performedwith RNeasy (Qiagen, Valencia,CA) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on affymetrix rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Daniel,,Savage
Sample_contact_email	dsavage@salud.unm.edu
Sample_contact_phone	505-272-8808
Sample_contact_fax	505-272-8082
Sample_contact_department	Neurosciences
Sample_contact_institute	University of New Mexico
Sample_contact_address	1University of New Mexico
Sample_contact_city	Albuquerque
Sample_contact_state	NM
Sample_contact_zip/postal_code	87131
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453980/suppl/GSM453980.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453980/suppl/GSM453980.CHP.gz
Sample_series_id	GSE18162
Sample_data_row_count	31099

GSM453981

GPL1355

070419_ETOH-34

placenta G20 ETOH

tissue: placenta agent: 5% ethanol in 0.066% saccharine solution

Gene expression data from placenta of ethanol consuming dams

Sample_geo_accession	GSM453981
Sample_status	Public on Jan 29 2010
Sample_submission_date	Sep 18 2009
Sample_last_update_date	Sep 18 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On Gestational day 20, placentae were removed by cesarian section, saline perfused, frozen in liquid nitrogen and stored at -80C
Sample_growth_protocol_ch1	Dams were consuming 5%ethanol in 0.066% saccharine solution or saccharine solution alone (controls) during their pregnancy, averaging 16ml during a 4hr interval every day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performedwith RNeasy (Qiagen, Valencia,CA) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on affymetrix rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Daniel,,Savage
Sample_contact_email	dsavage@salud.unm.edu
Sample_contact_phone	505-272-8808
Sample_contact_fax	505-272-8082
Sample_contact_department	Neurosciences
Sample_contact_institute	University of New Mexico
Sample_contact_address	1University of New Mexico
Sample_contact_city	Albuquerque
Sample_contact_state	NM
Sample_contact_zip/postal_code	87131
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453981/suppl/GSM453981.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453981/suppl/GSM453981.CHP.gz
Sample_series_id	GSE18162
Sample_data_row_count	31099

GSM453982

GPL1355

070419_ETOH-45

placenta G20 ETOH

tissue: placenta agent: 5% ethanol in 0.066% saccharine solution

Gene expression data from placenta of ethanol consuming dams

Sample_geo_accession	GSM453982
Sample_status	Public on Jan 29 2010
Sample_submission_date	Sep 18 2009
Sample_last_update_date	Sep 18 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On Gestational day 20, placentae were removed by cesarian section, saline perfused, frozen in liquid nitrogen and stored at -80C
Sample_growth_protocol_ch1	Dams were consuming 5%ethanol in 0.066% saccharine solution or saccharine solution alone (controls) during their pregnancy, averaging 16ml during a 4hr interval every day.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performedwith RNeasy (Qiagen, Valencia,CA) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on affymetrix rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Daniel,,Savage
Sample_contact_email	dsavage@salud.unm.edu
Sample_contact_phone	505-272-8808
Sample_contact_fax	505-272-8082
Sample_contact_department	Neurosciences
Sample_contact_institute	University of New Mexico
Sample_contact_address	1University of New Mexico
Sample_contact_city	Albuquerque
Sample_contact_state	NM
Sample_contact_zip/postal_code	87131
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453982/suppl/GSM453982.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM453nnn/GSM453982/suppl/GSM453982.CHP.gz
Sample_series_id	GSE18162
Sample_data_row_count	31099

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