Search results for the GEO ID: GSE18235 ![](/q11.jpeg) |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM455792 | GPL570 |
|
061006_Undosed control, biological rep1
|
Undosed control
|
cell type: Primary human bronchial/tracheal epithelial cells
|
Control_1
|
Sample_geo_accession | GSM455792
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455792/suppl/GSM455792.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455792/suppl/GSM455792.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455793 | GPL570 |
|
061006_Undosed control, biological rep2
|
Undosed control
|
cell type: Primary human bronchial/tracheal epithelial cells
|
Control_2
|
Sample_geo_accession | GSM455793
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455793/suppl/GSM455793.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455793/suppl/GSM455793.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455794 | GPL570 |
|
Dosed with CDC1 tobacco product, biological rep1
|
Dosed with CDC1 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC1_1
|
Sample_geo_accession | GSM455794
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455794/suppl/GSM455794.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455794/suppl/GSM455794.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455795 | GPL570 |
|
Dosed with CDC1 tobacco product, biological rep2
|
Dosed with CDC1 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC1_2
|
Sample_geo_accession | GSM455795
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455795/suppl/GSM455795.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455795/suppl/GSM455795.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455796 | GPL570 |
|
Dosed with CDC2 tobacco product, biological rep1
|
Dosed with CDC2 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC2_1
|
Sample_geo_accession | GSM455796
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455796/suppl/GSM455796.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455796/suppl/GSM455796.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455797 | GPL570 |
|
Dosed with CDC2 tobacco product, biological rep2
|
Dosed with CDC2 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC2_2
|
Sample_geo_accession | GSM455797
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455797/suppl/GSM455797.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455797/suppl/GSM455797.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455798 | GPL570 |
|
Dosed with CDC3 tobacco product, biological rep1
|
Dosed with CDC3 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC3_1
|
Sample_geo_accession | GSM455798
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455798/suppl/GSM455798.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455798/suppl/GSM455798.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455799 | GPL570 |
|
Dosed with CDC3 tobacco product, biological rep2
|
Dosed with CDC3 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC3_2
|
Sample_geo_accession | GSM455799
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455799/suppl/GSM455799.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455799/suppl/GSM455799.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455800 | GPL570 |
|
Dosed with CDC4 tobacco product, biological rep1
|
Dosed with CDC4 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC4_1
|
Sample_geo_accession | GSM455800
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455800/suppl/GSM455800.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455800/suppl/GSM455800.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455801 | GPL570 |
|
Dosed with CDC4 tobacco product, biological rep2
|
Dosed with CDC4 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC4_2
|
Sample_geo_accession | GSM455801
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455801/suppl/GSM455801.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455801/suppl/GSM455801.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455802 | GPL570 |
|
Dosed with CDC5 tobacco product, biological rep1
|
Dosed with CDC5 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC5_1
|
Sample_geo_accession | GSM455802
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455802/suppl/GSM455802.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455802/suppl/GSM455802.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455803 | GPL570 |
|
Dosed with CDC5 tobacco product, biological rep2
|
Dosed with CDC5 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC5_2
|
Sample_geo_accession | GSM455803
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455803/suppl/GSM455803.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455803/suppl/GSM455803.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455804 | GPL570 |
|
Dosed with CDC6 tobacco product, biological rep1
|
Dosed with CDC6 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC6_1
|
Sample_geo_accession | GSM455804
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455804/suppl/GSM455804.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455804/suppl/GSM455804.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455805 | GPL570 |
|
Dosed with CDC6 tobacco product, biological rep2
|
Dosed with CDC6 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC6_2
|
Sample_geo_accession | GSM455805
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455805/suppl/GSM455805.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455805/suppl/GSM455805.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455806 | GPL570 |
|
Dosed with CDC7 tobacco product, biological rep1
|
Dosed with CDC7 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC7_1
|
Sample_geo_accession | GSM455806
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455806/suppl/GSM455806.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455806/suppl/GSM455806.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455807 | GPL570 |
|
Dosed with CDC7 tobacco product, biological rep2
|
Dosed with CDC7 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC7_2
|
Sample_geo_accession | GSM455807
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455807/suppl/GSM455807.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455807/suppl/GSM455807.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455808 | GPL570 |
|
Dosed with CDC8 tobacco product, biological rep1
|
Dosed with CDC8 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC8_1
|
Sample_geo_accession | GSM455808
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455808/suppl/GSM455808.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455808/suppl/GSM455808.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455809 | GPL570 |
|
Dosed with CDC8 tobacco product, biological rep2
|
Dosed with CDC8 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC8_2
|
Sample_geo_accession | GSM455809
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455809/suppl/GSM455809.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455809/suppl/GSM455809.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455810 | GPL570 |
|
Dosed with CDC9 tobacco product, biological rep1
|
Dosed with CDC9 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC9_1
|
Sample_geo_accession | GSM455810
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455810/suppl/GSM455810.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455810/suppl/GSM455810.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455811 | GPL570 |
|
Dosed with CDC9 tobacco product, biological rep2
|
Dosed with CDC9 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC9_2
|
Sample_geo_accession | GSM455811
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455811/suppl/GSM455811.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455811/suppl/GSM455811.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455812 | GPL570 |
|
Dosed with CDC10 tobacco product, biological rep1
|
Dosed with CDC10 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC10_1
|
Sample_geo_accession | GSM455812
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455812/suppl/GSM455812.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455812/suppl/GSM455812.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
| |
|
GSM455813 | GPL570 |
|
Dosed with CDC10 tobacco product, biological rep2
|
Dosed with CDC10 tobacco product
|
cell type: Primary human bronchial/tracheal epithelial cells
|
CDC10_2
|
Sample_geo_accession | GSM455813
| Sample_status | Public on Sep 24 2009
| Sample_submission_date | Sep 23 2009
| Sample_last_update_date | Sep 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.
| Sample_growth_protocol_ch1 | Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human HGU133 2.0+ Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Gavin,,Pickett
| Sample_contact_laboratory | KUGR
| Sample_contact_department | Genomics
| Sample_contact_institute | UNM
| Sample_contact_address | 2325 Camino de Salud NE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87131
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455813/suppl/GSM455813.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455813/suppl/GSM455813.CHP.gz
| Sample_series_id | GSE18235
| Sample_data_row_count | 54675
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