Result

Search results for the GEO ID: GSE18244

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM455882

GPL1261

Control LLC lung

Lung of C57BL/6 mice injected with Lewis lung carcinoma (LLC) cells

tissue: lung strain: C57BL/6 agent: vector-transfected Lewis lung carcinoma cells

None.

Sample_geo_accession	GSM455882
Sample_status	Public on Sep 06 2010
Sample_submission_date	Sep 24 2009
Sample_last_update_date	Sep 03 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from murine whole lungs and purified using an RNeasy Mini Kit (Qiagen). The total lung RNA samples from ten mice were pooled.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	2µg of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5’-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3’ (Affymetrix). Double-stranded cDNA was cleaned up by using the GeneChip® Sample Cleanup Module (Affymetrix). In vitro transcription was performed on all of cDNA using the GeneChip® IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized (45°C, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip® Scanner 3000. Chip analysis was performed using the Affymetrix GeneChip® Operating Software v1.4.
Sample_data_processing	The scan values were normalized to make its average equal to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Satoshi,,Ishii
Sample_contact_email	mame@m.u-tokyo.ac.jp
Sample_contact_phone	+81-3-5082-2925
Sample_contact_fax	+81-3-3813-8732
Sample_contact_department	Department of Biochem. & Mol. Biol.
Sample_contact_institute	The University of Tokyo
Sample_contact_address	7-3-1 Hongo, Bunkyo
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-0033
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455882/suppl/GSM455882.CEL.gz
Sample_series_id	GSE18244
Sample_data_row_count	45101

GSM455883

GPL1261

TDAG8 LLC lung

Lung of C57BL/6 mice injected with Lewis lung carcinoma (LLC) cells

tissue: lung strain: C57BL/6 agent: TDAG8-overexpressing Lewis lung carcinoma cells

None.

Sample_geo_accession	GSM455883
Sample_status	Public on Sep 06 2010
Sample_submission_date	Sep 24 2009
Sample_last_update_date	Sep 03 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from murine whole lungs and purified using an RNeasy Mini Kit (Qiagen). The total lung RNA samples from ten mice were pooled.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	2µg of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5’-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3’ (Affymetrix). Double-stranded cDNA was cleaned up by using the GeneChip® Sample Cleanup Module (Affymetrix). In vitro transcription was performed on all of cDNA using the GeneChip® IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized (45°C, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip® Scanner 3000. Chip analysis was performed using the Affymetrix GeneChip® Operating Software v1.4.
Sample_data_processing	The scan values were normalized to make its average equal to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Satoshi,,Ishii
Sample_contact_email	mame@m.u-tokyo.ac.jp
Sample_contact_phone	+81-3-5082-2925
Sample_contact_fax	+81-3-3813-8732
Sample_contact_department	Department of Biochem. & Mol. Biol.
Sample_contact_institute	The University of Tokyo
Sample_contact_address	7-3-1 Hongo, Bunkyo
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	113-0033
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM455nnn/GSM455883/suppl/GSM455883.CEL.gz
Sample_series_id	GSE18244
Sample_data_row_count	45101

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