Search results for the GEO ID: GSE18326 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM457629 | GPL1261 |
|
FoxO3null_2_1
|
FoxO3 null mutant
|
strain: FVB/N
tissue: forebrain
|
A_F3 NS2 #1_Mouse430_2
|
Sample_geo_accession | GSM457629
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457629/suppl/GSM457629.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
GSM457630 | GPL1261 |
|
FoxO3null_2_2
|
FoxO3 null mutant
|
strain: FVB/N
tissue: forebrain
|
A_F3 NS2 #2_Mouse430_2
|
Sample_geo_accession | GSM457630
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457630/suppl/GSM457630.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
GSM457631 | GPL1261 |
|
WildType_2_1
|
wild type
|
strain: FVB/N
tissue: forebrain
|
A_WT NS2 #1_Mouse430_2
|
Sample_geo_accession | GSM457631
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457631/suppl/GSM457631.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
GSM457632 | GPL1261 |
|
WildType_2_2
|
wild type
|
strain: FVB/N
tissue: forebrain
|
A_WT NS2 #2_Mouse430_2
|
Sample_geo_accession | GSM457632
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457632/suppl/GSM457632.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
GSM457633 | GPL1261 |
|
FoxO3null_1_1
|
FoxO3 null mutant
|
strain: FVB/N
tissue: forebrain
|
B_NS2-Fox03null #1_Mouse430_2
|
Sample_geo_accession | GSM457633
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457633/suppl/GSM457633.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
GSM457634 | GPL1261 |
|
FoxO3null_1_2
|
FoxO3 null mutant
|
strain: FVB/N
tissue: forebrain
|
B_NS2-Fox03null #2_Mouse430_2
|
Sample_geo_accession | GSM457634
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457634/suppl/GSM457634.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
GSM457635 | GPL1261 |
|
WildType_1_1
|
wild type
|
strain: FVB/N
tissue: forebrain
|
B_NS2-WT #1_Mouse430_2
|
Sample_geo_accession | GSM457635
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457635/suppl/GSM457635.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
GSM457636 | GPL1261 |
|
WildType_1_2
|
wild type
|
strain: FVB/N
tissue: forebrain
|
B_NS2-WT #2_Mouse430_2
|
Sample_geo_accession | GSM457636
| Sample_status | Public on Nov 29 2009
| Sample_submission_date | Sep 29 2009
| Sample_last_update_date | Sep 29 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | NSC from neurospheres were enzymatically dissociated for 5-10 minutes at 37ºC with Accutase (Chemicon), then mechanically dissociated and seeded at a density of 5x104 cells/ml.
| Sample_growth_protocol_ch1 | Mouse forebrains were finely minced, digested for 30 minutes at 37ºC in HBSS media containing 2.5 U/ml Papain (Worthington), 1U/ml Dispase (Roche or Stem Cell Technologies), and 250 U/ml DNase I (Sigma) and mechanically dissociated. NSC/progenitors were purified using two successive Percoll gradients (25% then 65%) (Amersham) and plated on uncoated tissue culture dishes at a density of 105 cells/cm2 in NeuroBasal-A medium supplemented with penicillin (100 U/ml)-streptomycin (100 mg/ml)-glutamine (292 µg/ml), serum-free B27 supplement without vitamin A (2%), bFGF (20 ng/ml), and EGF (20 ng/ml) (self-renewal/proliferation media). Cells were incubated at 37ºC. Secondary neurospheres were collected 6 days after initial plating at 50000 cells/ml.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the mirVana isolation kit following the manufacturer’s protocol (Ambion)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin
| Sample_hyb_protocol | Microarray hybridization was performed at the Stanford PAN facility. RNA was reverse-transcribed to cDNA followed by in vitro transcription and biotinylation. Biotinylated cRNAs were then fragmented, mixed with control cRNA fragments, and hybridized to Affymetrix oligonucleotide arrays (Mouse Genome 430 2.0 Array).
| Sample_scan_protocol | Microarray scanning was performed at the Stanford PAN facility.
| Sample_data_processing | Microarray results were pre-processed by performing a background adjustment and normalization with RMA (robust multi-array analysis) using Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Alex,,Morgan
| Sample_contact_laboratory | Atul Butte Lab
| Sample_contact_department | Biomedical Informatics
| Sample_contact_institute | Stanford Medical School
| Sample_contact_address | 251 Campus Drive, MSOB X-215
| Sample_contact_city | Stanford
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 94305-5479
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.stanford.edu/~alexmo/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457636/suppl/GSM457636.CEL.gz
| Sample_series_id | GSE18326
| Sample_data_row_count | 45101
| |
|
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