Result

Search results for the GEO ID: GSE18327

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM457637

GPL1261

Wild_type_control

Entire small intestine, wild type, control

strain: C57BL/6 tissue: small intestine genotype: wild type treatment: none

WTI_070908

Sample_geo_accession	GSM457637
Sample_status	Public on Sep 20 2010
Sample_submission_date	Sep 29 2009
Sample_last_update_date	Sep 20 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were gavaged daily with 100 ul Peptamen including 10 ug/kg body weight lubiprostone for two weeks, as indicated.
Sample_growth_protocol_ch1	All mice were fed from weaning to sacrifice with a liquid diet (Peptamen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The entire small intestine was resected into ice cold PBS; the mesentery was trimmed and discarded; the tissue was homogenized in 8 ml Trizol and extracted to isolate total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The standard labeling protocol was used.
Sample_hyb_protocol	GeneChip was prehybridized with Hybridization buffer at 45C 10min.
Sample_hyb_protocol	Denatured Hybridization cocktail was applied to GeneChip.
Sample_hyb_protocol	GeneChip was hybridized 16 hr at 45C in GeneChip Hybridization Oven 640.
Sample_hyb_protocol	Washing and Staining: Automated process using the GeneChip Fluidics Station 400 with the Standard Array Format.
Sample_scan_protocol	Automated process conducted using the Agilent GeneArray Scanner.
Sample_scan_protocol = Pixel value	3um
Sample_scan_protocol = Wavelength	570nm
Sample_data_processing = Primary analysis was by GCOS with 'Scaling - All Probe Sets: Target Signal = 500'; 'Normalization – User Defined: Scale Factor	1' using MAS 5.0.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,C,De Lisle
Sample_contact_email	rdelisle@kumc.edu
Sample_contact_phone	913-588-2742
Sample_contact_department	Anatomy & Cell Biology
Sample_contact_institute	Univ Kansas Med Ctr
Sample_contact_address	3901 Rainbow Blvd
Sample_contact_city	Kansas City
Sample_contact_state	KS
Sample_contact_zip/postal_code	66160
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457637/suppl/GSM457637.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457637/suppl/GSM457637.CHP.gz
Sample_series_id	GSE18327
Sample_data_row_count	45101

GSM457638

GPL1261

Wild_type_lubiprostone

Entire small intestine, wild type, lubiprostone treated

strain: C57BL/6 tissue: small intestine genotype: wild type treatment: lubiprostone

WTIL_070908

Sample_geo_accession	GSM457638
Sample_status	Public on Sep 20 2010
Sample_submission_date	Sep 29 2009
Sample_last_update_date	Sep 20 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were gavaged daily with 100 ul Peptamen including 10 ug/kg body weight lubiprostone for two weeks, as indicated.
Sample_growth_protocol_ch1	All mice were fed from weaning to sacrifice with a liquid diet (Peptamen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The entire small intestine was resected into ice cold PBS; the mesentery was trimmed and discarded; the tissue was homogenized in 8 ml Trizol and extracted to isolate total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The standard labeling protocol was used.
Sample_hyb_protocol	GeneChip was prehybridized with Hybridization buffer at 45C 10min.
Sample_hyb_protocol	Denatured Hybridization cocktail was applied to GeneChip.
Sample_hyb_protocol	GeneChip was hybridized 16 hr at 45C in GeneChip Hybridization Oven 640.
Sample_hyb_protocol	Washing and Staining: Automated process using the GeneChip Fluidics Station 400 with the Standard Array Format.
Sample_scan_protocol	Automated process conducted using the Agilent GeneArray Scanner.
Sample_scan_protocol = Pixel value	3um
Sample_scan_protocol = Wavelength	570nm
Sample_data_processing = Primary analysis was by GCOS with 'Scaling - All Probe Sets: Target Signal = 500'; 'Normalization – User Defined: Scale Factor	1' using MAS 5.0.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,C,De Lisle
Sample_contact_email	rdelisle@kumc.edu
Sample_contact_phone	913-588-2742
Sample_contact_department	Anatomy & Cell Biology
Sample_contact_institute	Univ Kansas Med Ctr
Sample_contact_address	3901 Rainbow Blvd
Sample_contact_city	Kansas City
Sample_contact_state	KS
Sample_contact_zip/postal_code	66160
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457638/suppl/GSM457638.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457638/suppl/GSM457638.CHP.gz
Sample_series_id	GSE18327
Sample_data_row_count	45101

GSM457639

GPL1261

CF_control

Entire small intestine, CF, control

strain: C57BL/6 tissue: small intestine genotype: cftr-tm1Unc (cftr null) treatment: none

CFI_070908

Sample_geo_accession	GSM457639
Sample_status	Public on Sep 20 2010
Sample_submission_date	Sep 29 2009
Sample_last_update_date	Sep 20 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were gavaged daily with 100 ul Peptamen including 10 ug/kg body weight lubiprostone for two weeks, as indicated.
Sample_growth_protocol_ch1	All mice were fed from weaning to sacrifice with a liquid diet (Peptamen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The entire small intestine was resected into ice cold PBS; the mesentery was trimmed and discarded; the tissue was homogenized in 8 ml Trizol and extracted to isolate total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The standard labeling protocol was used.
Sample_hyb_protocol	GeneChip was prehybridized with Hybridization buffer at 45C 10min.
Sample_hyb_protocol	Denatured Hybridization cocktail was applied to GeneChip.
Sample_hyb_protocol	GeneChip was hybridized 16 hr at 45C in GeneChip Hybridization Oven 640.
Sample_hyb_protocol	Washing and Staining: Automated process using the GeneChip Fluidics Station 400 with the Standard Array Format.
Sample_scan_protocol	Automated process conducted using the Agilent GeneArray Scanner.
Sample_scan_protocol = Pixel value	3um
Sample_scan_protocol = Wavelength	570nm
Sample_data_processing = Primary analysis was by GCOS with 'Scaling - All Probe Sets: Target Signal = 500'; 'Normalization – User Defined: Scale Factor	1' using MAS 5.0.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,C,De Lisle
Sample_contact_email	rdelisle@kumc.edu
Sample_contact_phone	913-588-2742
Sample_contact_department	Anatomy & Cell Biology
Sample_contact_institute	Univ Kansas Med Ctr
Sample_contact_address	3901 Rainbow Blvd
Sample_contact_city	Kansas City
Sample_contact_state	KS
Sample_contact_zip/postal_code	66160
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457639/suppl/GSM457639.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457639/suppl/GSM457639.CHP.gz
Sample_series_id	GSE18327
Sample_data_row_count	45101

GSM457640

GPL1261

CF_lubiprostone

Entire small intestine, CF, lubiprostone treated

strain: C57BL/6 tissue: small intestine genotype: cftr-tm1Unc (cftr null) treatment: lubiprostone

CFIL_070908

Sample_geo_accession	GSM457640
Sample_status	Public on Sep 20 2010
Sample_submission_date	Sep 29 2009
Sample_last_update_date	Sep 20 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were gavaged daily with 100 ul Peptamen including 10 ug/kg body weight lubiprostone for two weeks, as indicated.
Sample_growth_protocol_ch1	All mice were fed from weaning to sacrifice with a liquid diet (Peptamen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The entire small intestine was resected into ice cold PBS; the mesentery was trimmed and discarded; the tissue was homogenized in 8 ml Trizol and extracted to isolate total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The standard labeling protocol was used.
Sample_hyb_protocol	GeneChip was prehybridized with Hybridization buffer at 45C 10min.
Sample_hyb_protocol	Denatured Hybridization cocktail was applied to GeneChip.
Sample_hyb_protocol	GeneChip was hybridized 16 hr at 45C in GeneChip Hybridization Oven 640.
Sample_hyb_protocol	Washing and Staining: Automated process using the GeneChip Fluidics Station 400 with the Standard Array Format.
Sample_scan_protocol	Automated process conducted using the Agilent GeneArray Scanner.
Sample_scan_protocol = Pixel value	3um
Sample_scan_protocol = Wavelength	570nm
Sample_data_processing = Primary analysis was by GCOS with 'Scaling - All Probe Sets: Target Signal = 500'; 'Normalization – User Defined: Scale Factor	1' using MAS 5.0.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,C,De Lisle
Sample_contact_email	rdelisle@kumc.edu
Sample_contact_phone	913-588-2742
Sample_contact_department	Anatomy & Cell Biology
Sample_contact_institute	Univ Kansas Med Ctr
Sample_contact_address	3901 Rainbow Blvd
Sample_contact_city	Kansas City
Sample_contact_state	KS
Sample_contact_zip/postal_code	66160
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457640/suppl/GSM457640.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM457nnn/GSM457640/suppl/GSM457640.CHP.gz
Sample_series_id	GSE18327
Sample_data_row_count	45101

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