Search results for the GEO ID: GSE18349  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM458064 | GPL570 |
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M031 CTX +7
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Human fetal cortex
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tissue: Fetal cortex
genotype: XY, trisomy 7
age: 94 day fetus
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Gene expression data from hNPCs containing a trisomy of chromosome 7
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| Sample_geo_accession | GSM458064
| | Sample_status | Public on Oct 01 2009
| | Sample_submission_date | Sep 30 2009
| | Sample_last_update_date | Sep 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Diploid and +7 human neural progenitor cells (hNPCs) were grown for approximately 25-30 weeks or 19-20 passages
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted trizol reagent. Total RNA was further extracted using a chloroform/isopropanol separation method. RNA was ethanol precipitated and allowed to briefly dry before elution in 33 ml of RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Stained U133 Plus 2.0 arrays were scanned at 3 μM resolution using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | GeneChip Operating Software v1.2 (GCOS) was used to analyze the relative abundance of each gene derived from the average difference of intensities.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dhruv,,Sareen
| | Sample_contact_email | dsareen@wisc.edu
| | Sample_contact_laboratory | Dr. Clive N. Svendsen Laboratory
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of Wisconsin Madison
| | Sample_contact_address | 1111 Highland Ave, Rm 5031 WIMR
| | Sample_contact_city | Madison
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53726
| | Sample_contact_country | USA
| | Sample_contact_web_link | stemcells.wisc.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458064/suppl/GSM458064.CEL.gz
| | Sample_series_id | GSE18349
| | Sample_data_row_count | 54675
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GSM458065 | GPL570 |
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M031 CTX diploid
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Human fetal cortex
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tissue: Fetal cortex
genotype: XY, diploid
age: 94 day fetus
|
Gene expression data from karyotypically normal hNPCs.
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| Sample_geo_accession | GSM458065
| | Sample_status | Public on Oct 01 2009
| | Sample_submission_date | Sep 30 2009
| | Sample_last_update_date | Sep 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Diploid and +7 human neural progenitor cells (hNPCs) were grown for approximately 25-30 weeks or 19-20 passages
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted trizol reagent. Total RNA was further extracted using a chloroform/isopropanol separation method. RNA was ethanol precipitated and allowed to briefly dry before elution in 33 ml of RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Stained U133 Plus 2.0 arrays were scanned at 3 μM resolution using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | GeneChip Operating Software v1.2 (GCOS) was used to analyze the relative abundance of each gene derived from the average difference of intensities.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dhruv,,Sareen
| | Sample_contact_email | dsareen@wisc.edu
| | Sample_contact_laboratory | Dr. Clive N. Svendsen Laboratory
| | Sample_contact_department | Neurology
| | Sample_contact_institute | University of Wisconsin Madison
| | Sample_contact_address | 1111 Highland Ave, Rm 5031 WIMR
| | Sample_contact_city | Madison
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53726
| | Sample_contact_country | USA
| | Sample_contact_web_link | stemcells.wisc.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458065/suppl/GSM458065.CEL.gz
| | Sample_series_id | GSE18349
| | Sample_data_row_count | 54675
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