Search results for the GEO ID: GSE18350  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM458070 | GPL570 |
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GM06690 replicate 1
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GM06690
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cell line: GM06690
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mRNA Expression
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| Sample_geo_accession | GSM458070
| | Sample_status | Public on Oct 01 2009
| | Sample_submission_date | Sep 30 2009
| | Sample_last_update_date | Sep 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human cell line GM06990, an EBV-transformed lymphoblastoid cell line (Coriell, Camden, NJ), was cultured in RPMI1640, 15% fetal calf serum, 1% penicillin-streptomycin, and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using QIAzol reagent and following the miRNeasy kit's procedure (Qiagen) and sample quality was controlled on a 2100 Bioanalyzer (Agilent).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | All samples were normalized to 125 ng/uL The Affymetrix One-Cycle Target labeling assay (P/N 900687) was used to synthesize hybridization target. Labeled cRNA hybridization target was produced using a 3-day, semi-automated workflow designed to maximize efficiency and reduce processing times. Samples were hybridized on GeneChip® Human Genome U133 Plus 2.0 arrays (P/N 900467).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | The washing and staining were performed on the GeneChip® Fluidics Station and the GeneChip® Scanner 3000 was used for scanning.
| | Sample_data_processing | After scanning, the expression value for each gene was calculated using RMA (Robust Multi-Array) normalization. The average intensity difference values were normalized across the sample set. Probe sets that were absent in all samples according to Affymetrix flags were removed. All values lower than 50 were replaced by 50.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Erez,,Lieberman-Aiden
| | Sample_contact_email | erez@erez.com
| | Sample_contact_institute | Broad Institute
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458070/suppl/GSM458070.CEL.gz
| | Sample_series_id | GSE18350
| | Sample_data_row_count | 36289
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GSM458071 | GPL570 |
|
GM06690 replicate 2
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GM06690
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cell line: GM06690
|
mRNA Expression
|
| Sample_geo_accession | GSM458071
| | Sample_status | Public on Oct 01 2009
| | Sample_submission_date | Sep 30 2009
| | Sample_last_update_date | Sep 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human cell line GM06990, an EBV-transformed lymphoblastoid cell line (Coriell, Camden, NJ), was cultured in RPMI1640, 15% fetal calf serum, 1% penicillin-streptomycin, and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using QIAzol reagent and following the miRNeasy kit's procedure (Qiagen) and sample quality was controlled on a 2100 Bioanalyzer (Agilent).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | All samples were normalized to 125 ng/uL The Affymetrix One-Cycle Target labeling assay (P/N 900687) was used to synthesize hybridization target. Labeled cRNA hybridization target was produced using a 3-day, semi-automated workflow designed to maximize efficiency and reduce processing times. Samples were hybridized on GeneChip® Human Genome U133 Plus 2.0 arrays (P/N 900467).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | The washing and staining were performed on the GeneChip® Fluidics Station and the GeneChip® Scanner 3000 was used for scanning.
| | Sample_data_processing | After scanning, the expression value for each gene was calculated using RMA (Robust Multi-Array) normalization. The average intensity difference values were normalized across the sample set. Probe sets that were absent in all samples according to Affymetrix flags were removed. All values lower than 50 were replaced by 50.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Erez,,Lieberman-Aiden
| | Sample_contact_email | erez@erez.com
| | Sample_contact_institute | Broad Institute
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458071/suppl/GSM458071.CEL.gz
| | Sample_series_id | GSE18350
| | Sample_data_row_count | 36289
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