Search results for the GEO ID: GSE18373  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM458345 | GPL570 |
|
HUVEC+MAGP2, Biological rep1
|
HUVEC cell line, MAGP2 treated
|
cell line: HUVEC
treatment: recombinant MAGP2
|
Gene expression data from HUVEC cells.
|
| Sample_geo_accession | GSM458345
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_HUVEC.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458345/suppl/GSM458345.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458346 | GPL570 |
|
HUVEC+MAGP2, Biological rep2
|
HUVEC cell line, MAGP2 treated
|
cell line: HUVEC
treatment: recombinant MAGP2
|
Gene expression data from HUVEC cells.
|
| Sample_geo_accession | GSM458346
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_HUVEC.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458346/suppl/GSM458346.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458347 | GPL570 |
|
HUVEC+MAGP2, Biological rep3
|
HUVEC cell line, MAGP2 treated
|
cell line: HUVEC
treatment: recombinant MAGP2
|
Gene expression data from HUVEC cells.
|
| Sample_geo_accession | GSM458347
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_HUVEC.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458347/suppl/GSM458347.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458348 | GPL570 |
|
HUVEC-MAGP2, Biological rep1
|
HUVEC cell line, untreated
|
cell line: HUVEC
treatment: untreated cells
|
Gene expression data from HUVEC cells.
|
| Sample_geo_accession | GSM458348
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_HUVEC.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458348/suppl/GSM458348.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458349 | GPL570 |
|
HUVEC-MAGP2, Biological rep2
|
HUVEC cell line, untreated
|
cell line: HUVEC
treatment: untreated cells
|
Gene expression data from HUVEC cells.
|
| Sample_geo_accession | GSM458349
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_HUVEC.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458349/suppl/GSM458349.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458350 | GPL570 |
|
HUVEC-MAGP2, Biological rep3
|
HUVEC cell line, untreated
|
cell line: HUVEC
treatment: untreated cells
|
Gene expression data from HUVEC cells.
|
| Sample_geo_accession | GSM458350
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_HUVEC.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458350/suppl/GSM458350.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458351 | GPL570 |
|
OVCA429+MAGP2, Biological rep1
|
OVCA429 cell line, MAGP2 treated
|
cell line: OVCA429
treatment: recombinant MAGP2
|
Gene expression data from OVCA429 cells.
|
| Sample_geo_accession | GSM458351
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_OVCA429.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458351/suppl/GSM458351.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458352 | GPL570 |
|
OVCA429+MAGP2, Biological rep2
|
OVCA429 cell line, MAGP2 treated
|
cell line: OVCA429
treatment: recombinant MAGP2
|
Gene expression data from OVCA429 cells.
|
| Sample_geo_accession | GSM458352
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_OVCA429.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458352/suppl/GSM458352.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458353 | GPL570 |
|
OVCA429+MAGP2, Biological rep3
|
OVCA429 cell line, MAGP2 treated
|
cell line: OVCA429
treatment: recombinant MAGP2
|
Gene expression data from OVCA429 cells.
|
| Sample_geo_accession | GSM458353
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_OVCA429.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458353/suppl/GSM458353.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458354 | GPL570 |
|
OVCA429-MAGP2, Biological rep1
|
OVCA429 cell line, untreated
|
cell line: OVCA429
treatment: untreated cells
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Gene expression data from OVCA429 cells.
|
| Sample_geo_accession | GSM458354
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_OVCA429.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458354/suppl/GSM458354.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458355 | GPL570 |
|
OVCA429-MAGP2, Biological rep2
|
OVCA429 cell line, untreated
|
cell line: OVCA429
treatment: untreated cells
|
Gene expression data from OVCA429 cells.
|
| Sample_geo_accession | GSM458355
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_OVCA429.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458355/suppl/GSM458355.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
| | |
|
GSM458356 | GPL570 |
|
OVCA429-MAGP2, Biological rep3
|
OVCA429 cell line, untreated
|
cell line: OVCA429
treatment: untreated cells
|
Gene expression data from OVCA429 cells.
|
| Sample_geo_accession | GSM458356
| | Sample_status | Public on Oct 17 2009
| | Sample_submission_date | Oct 01 2009
| | Sample_last_update_date | Oct 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | recMAGP2 treated and untreated cell lines.
| | Sample_growth_protocol_ch1 | OVCA429 were grown in RPMI-1640 supplemented with 10% FBS, 2mM glutamine and penicillin/streptomycin (Invitrogen). HUVECs were grown in EBM supplemented with 2% FBS and EGM-2 MV SingleQuots.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini Kit, Qiagen.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Two rounds of amplification were completed according to standard Affymetrix Two-Cycle Amplification protocol using 25ng of total RNA.
| | Sample_hyb_protocol | A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Biometric Research Branch (BRB) ArrayTools version 3.2.2 software developed by Dr. Richard Simon and Amy Peng Lam of the Biometrics Research Branch of the National Cancer Institute was used to filter and complete the statistical analysis. Only those probe sets present in greater than 50% of the arrays and displaying a variance in the top 50th percentile were evaluated. Differentially expressed genes were identified by a univariate t-test (P<0.01 and fold-change>1.5). A random-variance model was selected to permit information sharing among probe sets.
| | Sample_data_processing |
| | Sample_data_processing | The differentially expressed genes are available in the file 'GSE18373_diff_exp_OVCA429.txt', which is linked to the Series GSE18373 record as a supplementary file.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Birrer
| | Sample_contact_email | mbirrer@partners.org
| | Sample_contact_phone | 6177244800
| | Sample_contact_laboratory | Surgical Oncology Research Labs
| | Sample_contact_department | Medicine
| | Sample_contact_institute | MGH
| | Sample_contact_address | 70 Blossom Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458356/suppl/GSM458356.CEL.gz
| | Sample_series_id | GSE18373
| | Sample_series_id | GSE18521
| | Sample_data_row_count | 54675
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