Result

Search results for the GEO ID: GSE18383

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Title

Source name

Description

Characteristics

GSM458547

GPL1261

p300+/- Rep1

hippocampal tissue from heterozygous p300 mice

genotype: p300+/- replicate: 1 strain: C57BL/6 gender: female age: adult tissue: hippocampus

Gene expression data from hippocampal tissue

Sample_geo_accession	GSM458547
Sample_status	Public on Oct 03 2009
Sample_submission_date	Oct 02 2009
Sample_last_update_date	Oct 02 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
Sample_growth_protocol_ch1	P300 deficient heterozygous mice were maintained in a C57BL/6J pure background. Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by a cleanup step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to Mouse Genome 430 2.0 genechips (Affymetrix, Santa Clara, CA). Three independent biological replicates were prepared for each genotype (wild-type and p300 deficient mice (p300+/-)). Mouse Genome 430 2.0 genechips were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Following Affymetrix instructions
Sample_hyb_protocol	Following Affymetrix instructions
Sample_scan_protocol	Following Affymetrix instructions
Sample_data_processing	GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of all samples. Data are represented as log2.
Sample_platform_id	GPL1261
Sample_contact_name	jose,p,lopez
Sample_contact_email	jose.lopez@umh.es
Sample_contact_department	Instituto Neurociencias Alicante
Sample_contact_institute	CSIC-UMH
Sample_contact_address	Av Ramon y Cajal s/n
Sample_contact_city	San Joan d'Alacant
Sample_contact_zip/postal_code	03550
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458547/suppl/GSM458547.CEL.gz
Sample_series_id	GSE18383
Sample_data_row_count	45101

GSM458548

GPL1261

p300+/- Rep2

hippocampal tissue from heterozygous p300 mice

genotype: p300+/- replicate: 2 strain: C57BL/6 gender: female age: adult tissue: hippocampus

Gene expression data from hippocampal tissue

Sample_geo_accession	GSM458548
Sample_status	Public on Oct 03 2009
Sample_submission_date	Oct 02 2009
Sample_last_update_date	Oct 02 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
Sample_growth_protocol_ch1	P300 deficient heterozygous mice were maintained in a C57BL/6J pure background. Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by a cleanup step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to Mouse Genome 430 2.0 genechips (Affymetrix, Santa Clara, CA). Three independent biological replicates were prepared for each genotype (wild-type and p300 deficient mice (p300+/-)). Mouse Genome 430 2.0 genechips were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Following Affymetrix instructions
Sample_hyb_protocol	Following Affymetrix instructions
Sample_scan_protocol	Following Affymetrix instructions
Sample_data_processing	GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of all samples. Data are represented as log2.
Sample_platform_id	GPL1261
Sample_contact_name	jose,p,lopez
Sample_contact_email	jose.lopez@umh.es
Sample_contact_department	Instituto Neurociencias Alicante
Sample_contact_institute	CSIC-UMH
Sample_contact_address	Av Ramon y Cajal s/n
Sample_contact_city	San Joan d'Alacant
Sample_contact_zip/postal_code	03550
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458548/suppl/GSM458548.CEL.gz
Sample_series_id	GSE18383
Sample_data_row_count	45101

GSM458549

GPL1261

p300+/- Rep3

hippocampal tissue from heterozygous p300 mice

genotype: p300+/- replicate: 3 strain: C57BL/6 gender: female age: adult tissue: hippocampus

Gene expression data from hippocampal tissue

Sample_geo_accession	GSM458549
Sample_status	Public on Oct 03 2009
Sample_submission_date	Oct 02 2009
Sample_last_update_date	Oct 02 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
Sample_growth_protocol_ch1	P300 deficient heterozygous mice were maintained in a C57BL/6J pure background. Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by a cleanup step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to Mouse Genome 430 2.0 genechips (Affymetrix, Santa Clara, CA). Three independent biological replicates were prepared for each genotype (wild-type and p300 deficient mice (p300+/-)). Mouse Genome 430 2.0 genechips were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Following Affymetrix instructions
Sample_hyb_protocol	Following Affymetrix instructions
Sample_scan_protocol	Following Affymetrix instructions
Sample_data_processing	GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of all samples. Data are represented as log2.
Sample_platform_id	GPL1261
Sample_contact_name	jose,p,lopez
Sample_contact_email	jose.lopez@umh.es
Sample_contact_department	Instituto Neurociencias Alicante
Sample_contact_institute	CSIC-UMH
Sample_contact_address	Av Ramon y Cajal s/n
Sample_contact_city	San Joan d'Alacant
Sample_contact_zip/postal_code	03550
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458549/suppl/GSM458549.CEL.gz
Sample_series_id	GSE18383
Sample_data_row_count	45101

GSM458550

GPL1261

Wild-type Rep1

hippocampal tissue from wild-type mice

genotype: Wild-type replicate: 1 strain: C57BL/6 gender: female age: adult tissue: hippocampus

Gene expression data from hippocampal tissue

Sample_geo_accession	GSM458550
Sample_status	Public on Oct 03 2009
Sample_submission_date	Oct 02 2009
Sample_last_update_date	Oct 02 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
Sample_growth_protocol_ch1	P300 deficient heterozygous mice were maintained in a C57BL/6J pure background. Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by a cleanup step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to Mouse Genome 430 2.0 genechips (Affymetrix, Santa Clara, CA). Three independent biological replicates were prepared for each genotype (wild-type and p300 deficient mice (p300+/-)). Mouse Genome 430 2.0 genechips were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Following Affymetrix instructions
Sample_hyb_protocol	Following Affymetrix instructions
Sample_scan_protocol	Following Affymetrix instructions
Sample_data_processing	GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of all samples. Data are represented as log2.
Sample_platform_id	GPL1261
Sample_contact_name	jose,p,lopez
Sample_contact_email	jose.lopez@umh.es
Sample_contact_department	Instituto Neurociencias Alicante
Sample_contact_institute	CSIC-UMH
Sample_contact_address	Av Ramon y Cajal s/n
Sample_contact_city	San Joan d'Alacant
Sample_contact_zip/postal_code	03550
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458550/suppl/GSM458550.CEL.gz
Sample_series_id	GSE18383
Sample_data_row_count	45101

GSM458551

GPL1261

Wild-type Rep2

hippocampal tissue from wild-type mice

genotype: Wild-type replicate: 2 strain: C57BL/6 gender: female age: adult tissue: hippocampus

Gene expression data from hippocampal tissue

Sample_geo_accession	GSM458551
Sample_status	Public on Oct 03 2009
Sample_submission_date	Oct 02 2009
Sample_last_update_date	Oct 02 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
Sample_growth_protocol_ch1	P300 deficient heterozygous mice were maintained in a C57BL/6J pure background. Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by a cleanup step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to Mouse Genome 430 2.0 genechips (Affymetrix, Santa Clara, CA). Three independent biological replicates were prepared for each genotype (wild-type and p300 deficient mice (p300+/-)). Mouse Genome 430 2.0 genechips were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Following Affymetrix instructions
Sample_hyb_protocol	Following Affymetrix instructions
Sample_scan_protocol	Following Affymetrix instructions
Sample_data_processing	GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of all samples. Data are represented as log2.
Sample_platform_id	GPL1261
Sample_contact_name	jose,p,lopez
Sample_contact_email	jose.lopez@umh.es
Sample_contact_department	Instituto Neurociencias Alicante
Sample_contact_institute	CSIC-UMH
Sample_contact_address	Av Ramon y Cajal s/n
Sample_contact_city	San Joan d'Alacant
Sample_contact_zip/postal_code	03550
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458551/suppl/GSM458551.CEL.gz
Sample_series_id	GSE18383
Sample_data_row_count	45101

GSM458552

GPL1261

Wild-type Rep3

hippocampal tissue from wild-type mice

genotype: Wild-type replicate: 3 strain: C57BL/6 gender: female age: adult tissue: hippocampus

Gene expression data from hippocampal tissue

Sample_geo_accession	GSM458552
Sample_status	Public on Oct 03 2009
Sample_submission_date	Oct 02 2009
Sample_last_update_date	Oct 02 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
Sample_growth_protocol_ch1	P300 deficient heterozygous mice were maintained in a C57BL/6J pure background. Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by a cleanup step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to Mouse Genome 430 2.0 genechips (Affymetrix, Santa Clara, CA). Three independent biological replicates were prepared for each genotype (wild-type and p300 deficient mice (p300+/-)). Mouse Genome 430 2.0 genechips were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Following Affymetrix instructions
Sample_hyb_protocol	Following Affymetrix instructions
Sample_scan_protocol	Following Affymetrix instructions
Sample_data_processing	GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of all samples. Data are represented as log2.
Sample_platform_id	GPL1261
Sample_contact_name	jose,p,lopez
Sample_contact_email	jose.lopez@umh.es
Sample_contact_department	Instituto Neurociencias Alicante
Sample_contact_institute	CSIC-UMH
Sample_contact_address	Av Ramon y Cajal s/n
Sample_contact_city	San Joan d'Alacant
Sample_contact_zip/postal_code	03550
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM458nnn/GSM458552/suppl/GSM458552.CEL.gz
Sample_series_id	GSE18383
Sample_data_row_count	45101

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