Search results for the GEO ID: GSE18476 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM460066 | GPL570 |
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U937T:PR no induction 48hr rep1
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U937T cells before induction
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cell line: U937T:PLZF-RARa
protocol: control
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Gene expression data from uninduced U937T:PLZFRARa cells
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Sample_geo_accession | GSM460066
| Sample_status | Public on Nov 30 2009
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Oct 08 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | PLZF-RARα expression was induced by washing cells three times with PBS and culturing in growth media without tetracycline.
| Sample_growth_protocol_ch1 | Cells were maintained in growth media: RPMI-1640 media supplemented with 10%(v/v) FBS, 1x Pen/Strep, puromycin (0.5ug/ml), G418 (1mg/ml) and tetracycline (0.1ug/ml)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using Qiagen Rneasy kit (Qiagen, Valencia, CA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | RNA was biotin labeled using the MessageAmpTMII kit (Ambion, Austin, TX).
| Sample_hyb_protocol | Following fragmentation, 10µg of cRNA was hybridized to U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the Perkin-Elmer Scanarray HT
| Sample_data_processing | Data was analyzed using ArrayAssistTM Version 5.2.2 (Stratagene, La Jolla, CA) and normalization was performed using the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Kim ,Lee,Rice
| Sample_contact_email | k-rice@northwestern.edu
| Sample_contact_phone | 312 503 1303
| Sample_contact_laboratory | Licht Lab
| Sample_contact_department | Hematology/Oncology
| Sample_contact_institute | Northwestern University
| Sample_contact_address | 303E Superior Street
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60611
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460066/suppl/GSM460066.CEL.gz
| Sample_series_id | GSE18476
| Sample_data_row_count | 54675
| |
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GSM460067 | GPL570 |
|
U937T:PR no induction 48hr rep2
|
U937T cells before induction
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cell line: U937T:PLZF-RARa
protocol: control
|
Gene expression data from uninduced U937T:PLZFRARa cells
|
Sample_geo_accession | GSM460067
| Sample_status | Public on Nov 30 2009
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Oct 08 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | PLZF-RARα expression was induced by washing cells three times with PBS and culturing in growth media without tetracycline.
| Sample_growth_protocol_ch1 | Cells were maintained in growth media: RPMI-1640 media supplemented with 10%(v/v) FBS, 1x Pen/Strep, puromycin (0.5ug/ml), G418 (1mg/ml) and tetracycline (0.1ug/ml)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using Qiagen Rneasy kit (Qiagen, Valencia, CA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | RNA was biotin labeled using the MessageAmpTMII kit (Ambion, Austin, TX).
| Sample_hyb_protocol | Following fragmentation, 10µg of cRNA was hybridized to U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the Perkin-Elmer Scanarray HT
| Sample_data_processing | Data was analyzed using ArrayAssistTM Version 5.2.2 (Stratagene, La Jolla, CA) and normalization was performed using the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Kim ,Lee,Rice
| Sample_contact_email | k-rice@northwestern.edu
| Sample_contact_phone | 312 503 1303
| Sample_contact_laboratory | Licht Lab
| Sample_contact_department | Hematology/Oncology
| Sample_contact_institute | Northwestern University
| Sample_contact_address | 303E Superior Street
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60611
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460067/suppl/GSM460067.CEL.gz
| Sample_series_id | GSE18476
| Sample_data_row_count | 54675
| |
|
GSM460068 | GPL570 |
|
U937T:PR no induction 48hr rep3
|
U937T cells before induction
|
cell line: U937T:PLZF-RARa
protocol: control
|
Gene expression data from uninduced U937T:PLZFRARa cells
|
Sample_geo_accession | GSM460068
| Sample_status | Public on Nov 30 2009
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Oct 08 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | PLZF-RARα expression was induced by washing cells three times with PBS and culturing in growth media without tetracycline.
| Sample_growth_protocol_ch1 | Cells were maintained in growth media: RPMI-1640 media supplemented with 10%(v/v) FBS, 1x Pen/Strep, puromycin (0.5ug/ml), G418 (1mg/ml) and tetracycline (0.1ug/ml)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using Qiagen Rneasy kit (Qiagen, Valencia, CA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | RNA was biotin labeled using the MessageAmpTMII kit (Ambion, Austin, TX).
| Sample_hyb_protocol | Following fragmentation, 10µg of cRNA was hybridized to U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the Perkin-Elmer Scanarray HT
| Sample_data_processing | Data was analyzed using ArrayAssistTM Version 5.2.2 (Stratagene, La Jolla, CA) and normalization was performed using the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Kim ,Lee,Rice
| Sample_contact_email | k-rice@northwestern.edu
| Sample_contact_phone | 312 503 1303
| Sample_contact_laboratory | Licht Lab
| Sample_contact_department | Hematology/Oncology
| Sample_contact_institute | Northwestern University
| Sample_contact_address | 303E Superior Street
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60611
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460068/suppl/GSM460068.CEL.gz
| Sample_series_id | GSE18476
| Sample_data_row_count | 54675
| |
|
GSM460069 | GPL570 |
|
U937T:PR induced 48hr rep1
|
U937T cells after PLZFRARa induction
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cell line: U937T:PLZF-RARa
protocol: PLZF-RARα expression induced by tetracycline removal for 48hr
|
Gene expression data from U937T:PLZFRARa cells induced by tetracycline removal for 48hr
|
Sample_geo_accession | GSM460069
| Sample_status | Public on Nov 30 2009
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Oct 08 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | PLZF-RARα expression was induced by washing cells three times with PBS and culturing in growth media without tetracycline.
| Sample_growth_protocol_ch1 | Cells were maintained in growth media: RPMI-1640 media supplemented with 10%(v/v) FBS, 1x Pen/Strep, puromycin (0.5ug/ml), G418 (1mg/ml) and tetracycline (0.1ug/ml)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using Qiagen Rneasy kit (Qiagen, Valencia, CA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | RNA was biotin labeled using the MessageAmpTMII kit (Ambion, Austin, TX).
| Sample_hyb_protocol | Following fragmentation, 10µg of cRNA was hybridized to U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the Perkin-Elmer Scanarray HT
| Sample_data_processing | Data was analyzed using ArrayAssistTM Version 5.2.2 (Stratagene, La Jolla, CA) and normalization was performed using the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Kim ,Lee,Rice
| Sample_contact_email | k-rice@northwestern.edu
| Sample_contact_phone | 312 503 1303
| Sample_contact_laboratory | Licht Lab
| Sample_contact_department | Hematology/Oncology
| Sample_contact_institute | Northwestern University
| Sample_contact_address | 303E Superior Street
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60611
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460069/suppl/GSM460069.CEL.gz
| Sample_series_id | GSE18476
| Sample_data_row_count | 54675
| |
|
GSM460070 | GPL570 |
|
U937T:PR induced 48hr rep2
|
U937T cells after PLZFRARa induction
|
cell line: U937T:PLZF-RARa
protocol: PLZF-RARα expression induced by tetracycline removal for 48hr
|
Gene expression data from U937T:PLZFRARa cells induced by tetracycline removal for 48hr
|
Sample_geo_accession | GSM460070
| Sample_status | Public on Nov 30 2009
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Oct 08 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | PLZF-RARα expression was induced by washing cells three times with PBS and culturing in growth media without tetracycline.
| Sample_growth_protocol_ch1 | Cells were maintained in growth media: RPMI-1640 media supplemented with 10%(v/v) FBS, 1x Pen/Strep, puromycin (0.5ug/ml), G418 (1mg/ml) and tetracycline (0.1ug/ml)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using Qiagen Rneasy kit (Qiagen, Valencia, CA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | RNA was biotin labeled using the MessageAmpTMII kit (Ambion, Austin, TX).
| Sample_hyb_protocol | Following fragmentation, 10µg of cRNA was hybridized to U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the Perkin-Elmer Scanarray HT
| Sample_data_processing | Data was analyzed using ArrayAssistTM Version 5.2.2 (Stratagene, La Jolla, CA) and normalization was performed using the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Kim ,Lee,Rice
| Sample_contact_email | k-rice@northwestern.edu
| Sample_contact_phone | 312 503 1303
| Sample_contact_laboratory | Licht Lab
| Sample_contact_department | Hematology/Oncology
| Sample_contact_institute | Northwestern University
| Sample_contact_address | 303E Superior Street
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60611
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460070/suppl/GSM460070.CEL.gz
| Sample_series_id | GSE18476
| Sample_data_row_count | 54675
| |
|
GSM460071 | GPL570 |
|
U937T:PR induced 48hr rep3
|
U937T cells after PLZFRARa induction
|
cell line: U937T:PLZF-RARa
protocol: PLZF-RARα expression induced by tetracycline removal for 48hr
|
Gene expression data from U937T:PLZFRARa cells induced by tetracycline removal for 48hr
|
Sample_geo_accession | GSM460071
| Sample_status | Public on Nov 30 2009
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Oct 08 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | PLZF-RARα expression was induced by washing cells three times with PBS and culturing in growth media without tetracycline.
| Sample_growth_protocol_ch1 | Cells were maintained in growth media: RPMI-1640 media supplemented with 10%(v/v) FBS, 1x Pen/Strep, puromycin (0.5ug/ml), G418 (1mg/ml) and tetracycline (0.1ug/ml)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using Qiagen Rneasy kit (Qiagen, Valencia, CA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | RNA was biotin labeled using the MessageAmpTMII kit (Ambion, Austin, TX).
| Sample_hyb_protocol | Following fragmentation, 10µg of cRNA was hybridized to U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the Perkin-Elmer Scanarray HT
| Sample_data_processing | Data was analyzed using ArrayAssistTM Version 5.2.2 (Stratagene, La Jolla, CA) and normalization was performed using the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Kim ,Lee,Rice
| Sample_contact_email | k-rice@northwestern.edu
| Sample_contact_phone | 312 503 1303
| Sample_contact_laboratory | Licht Lab
| Sample_contact_department | Hematology/Oncology
| Sample_contact_institute | Northwestern University
| Sample_contact_address | 303E Superior Street
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60611
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460071/suppl/GSM460071.CEL.gz
| Sample_series_id | GSE18476
| Sample_data_row_count | 54675
| |
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