Search results for the GEO ID: GSE18486 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM460255 | GPL570 |
|
A2780 untreated, rep1
|
A2780, untreated
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: Untreated
|
Gene expression data from A2780 untreated.
|
Sample_geo_accession | GSM460255
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460255/suppl/GSM460255.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460256 | GPL570 |
|
A2780 untreated, rep2
|
A2780, untreated
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: Untreated
|
Gene expression data from A2780 untreated.
|
Sample_geo_accession | GSM460256
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460256/suppl/GSM460256.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460257 | GPL570 |
|
A2780 untreated, rep3
|
A2780, untreated
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: Untreated
|
Gene expression data from A2780 untreated.
|
Sample_geo_accession | GSM460257
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460257/suppl/GSM460257.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460258 | GPL570 |
|
A2780 treated with CDk-125, rep1
|
A2780, CDk-125
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: CDk-125
|
Gene expression data from A2780 treated with CDK-125.
|
Sample_geo_accession | GSM460258
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460258/suppl/GSM460258.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460259 | GPL570 |
|
A2780 treated with CDk-125, rep2
|
A2780, CDk-125
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: CDk-125
|
Gene expression data from A2780 treated with CDK-125.
|
Sample_geo_accession | GSM460259
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460259/suppl/GSM460259.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460260 | GPL570 |
|
A2780 treated with CDk-125, rep3
|
A2780, CDk-125
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: CDk-125
|
Gene expression data from A2780 treated with CDK-125.
|
Sample_geo_accession | GSM460260
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460260/suppl/GSM460260.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460261 | GPL570 |
|
A2780 treated with CDk-509, rep1
|
A2780, CDk-509
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: CDk-509
|
Gene expression data from A2780 treated with CDK-509.
|
Sample_geo_accession | GSM460261
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460261/suppl/GSM460261.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460262 | GPL570 |
|
A2780 treated with CDk-509, rep2
|
A2780, CDk-509
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: CDk-509
|
Gene expression data from A2780 treated with CDK-509.
|
Sample_geo_accession | GSM460262
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460262/suppl/GSM460262.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM460263 | GPL570 |
|
A2780 treated with CDk-509, rep3
|
A2780, CDk-509
|
cell line: A2780
cell type: adenocarcinoma ovary
treatment: CDk-509
|
Gene expression data from A2780 treated with CDK-509.
|
Sample_geo_accession | GSM460263
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 08 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after seeding, cells were treated with different compounds at a dose equal to 5 time the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | A2780 (human adenocarcinoma ovary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM460nnn/GSM460263/suppl/GSM460263.CEL.gz
| Sample_series_id | GSE18486
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
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