Search results for the GEO ID: GSE18501 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM461114 | GPL570 |
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MCF7 untreated, rep1
|
MCF7, untreated
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cell line: MCF7
cell type: adenocarcinoma mammary cells
treatment: untreated
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Gene expression data from MCF7 untreated.
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Sample_geo_accession | GSM461114
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 09 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | After 24 hours, cells were treated with the compound at a dose equal to 5 times the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | MCF7 (human adenocarcinoma mammary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as per manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM461nnn/GSM461114/suppl/GSM461114.CEL.gz
| Sample_series_id | GSE18501
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM461115 | GPL570 |
|
MCF7 untreated, rep2
|
MCF7, untreated
|
cell line: MCF7
cell type: adenocarcinoma mammary cells
treatment: untreated
|
Gene expression data from MCF7 untreated.
|
Sample_geo_accession | GSM461115
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 09 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | After 24 hours, cells were treated with the compound at a dose equal to 5 times the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | MCF7 (human adenocarcinoma mammary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as per manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM461nnn/GSM461115/suppl/GSM461115.CEL.gz
| Sample_series_id | GSE18501
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM461116 | GPL570 |
|
MCF7 treated with CDK-125, rep1
|
MCF7, CDK-125
|
cell line: MCF7
cell type: adenocarcinoma mammary cells
treatment: CDK-125
|
Gene expression data from MCF7 treated with CDK-125.
|
Sample_geo_accession | GSM461116
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 09 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | After 24 hours, cells were treated with the compound at a dose equal to 5 times the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | MCF7 (human adenocarcinoma mammary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as per manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM461nnn/GSM461116/suppl/GSM461116.CEL.gz
| Sample_series_id | GSE18501
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
|
GSM461117 | GPL570 |
|
MCF7 treated with CDK-125, rep2
|
MCF7, CDK-125
|
cell line: MCF7
cell type: adenocarcinoma mammary cells
treatment: CDK-125
|
Gene expression data from MCF7 treated with CDK-125.
|
Sample_geo_accession | GSM461117
| Sample_status | Public on Jul 14 2010
| Sample_submission_date | Oct 09 2009
| Sample_last_update_date | Jul 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | After 24 hours, cells were treated with the compound at a dose equal to 5 times the IC50 for 6 hours and collected using Qiagen lysis buffer RLT (Qiagen cat no. 79216).
| Sample_growth_protocol_ch1 | MCF7 (human adenocarcinoma mammary) from ECACC were seeded in T-75 tissue culture flasks (Corning), 25.000 cells/cm2 in RPMI 1640 (Gibco), pH 7.4, 10% FBS (EUROCLONE Australia-USDA approved, cat. ECSO170D, lot.EUS003383), 2mM L-Glutamine (Gibco), 1x Penicillin-Streptomycin (Gibco) and maintained in 5% CO2 at 37°C with 96% relative humidity.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy kit (Qiagen cat. no. 74104), starting from total cell lysates.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled, fragmented cRNA probes were prepared starting from 1.5 µg of total RNA per replicate sample, using the ‘One-Cycle Target Labeling and Control Reagents’ (Affymetrix, Santa Clara, CA) according to the protocols included in the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
| Sample_hyb_protocol | Samples were hybridized onto Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays and processed as per manufacturer’s instructions using ‘GeneChip® Hybridization, Wash, and Stain Kit’ components (Affymetrix, Santa Clara, CA).
| Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000.
| Sample_data_processing | Scanned images were first inspected for quality control (QC) using a variety of built-in QC tools from the Bioconductor package [www.bioconductor.org] of R, the open source environment for statistical analysis. Feature intensity values from scanned arrays were normalised and reduced to expression summaries using MAS5 implemented in the R statistical environment.
| Sample_platform_id | GPL570
| Sample_contact_name | Roberta,,Bosotti
| Sample_contact_email | roberta.bosotti@nervianoms.com
| Sample_contact_laboratory | Genomics
| Sample_contact_department | Biotechnology
| Sample_contact_institute | Nerviano Medical Sciences srl
| Sample_contact_address | viale Pasteur,10
| Sample_contact_city | Nerviano
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 20014
| Sample_contact_country | Italy
| Sample_contact_web_link | http://www.nervianoms.com/cont/en/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM461nnn/GSM461117/suppl/GSM461117.CEL.gz
| Sample_series_id | GSE18501
| Sample_series_id | GSE18552
| Sample_data_row_count | 54675
| |
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