Search results for the GEO ID: GSE18599  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM462492 | GPL1261 |
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L1-GL3-1
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differentiating 3T3-L1 cells with control siRNA
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cell type: differentiating 3T3-L1 adipocytes
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3T3-L1, control siRNA
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| Sample_geo_accession | GSM462492
| | Sample_status | Public on Apr 05 2012
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Apr 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were washed twice with PBS, and were collected in cell lysis buffer RLT (RNeasy Mini Kit) and homogenized.
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM containing 25mM glucose and 10% FBS. For adipogenic induction, insulin, dexamethaone and 3- isobutyl-1-methylxanthine were added.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsuyoshi,,Nakao
| | Sample_contact_email | mnakao@gpo.kumamoto-u.ac.jp
| | Sample_contact_institute | Kumamoto University
| | Sample_contact_address | 2-2-1 Honjo
| | Sample_contact_city | Kumamoto
| | Sample_contact_zip/postal_code | 860-0811
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462492/suppl/GSM462492_L1-GL3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462492/suppl/GSM462492_L1-GL3.mas5.CHP.gz
| | Sample_series_id | GSE18599
| | Sample_series_id | GSE18600
| | Sample_data_row_count | 45101
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GSM462493 | GPL1261 |
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L1-PHF21A
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differentiating 3T3-L1 cells with phf21a-specific siRNA
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cell type: differentiating 3T3-L1 adipocytes
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3T3-L1, phf21a siRNA
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| Sample_geo_accession | GSM462493
| | Sample_status | Public on Apr 05 2012
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Apr 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were washed twice with PBS, and were collected in cell lysis buffer RLT (RNeasy Mini Kit) and homogenized.
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM containing 25mM glucose and 10% FBS. For adipogenic induction, insulin, dexamethaone and 3- isobutyl-1-methylxanthine were added.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsuyoshi,,Nakao
| | Sample_contact_email | mnakao@gpo.kumamoto-u.ac.jp
| | Sample_contact_institute | Kumamoto University
| | Sample_contact_address | 2-2-1 Honjo
| | Sample_contact_city | Kumamoto
| | Sample_contact_zip/postal_code | 860-0811
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462493/suppl/GSM462493_L1-PHF21A.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462493/suppl/GSM462493_L1-PHF21A.mas5.CHP.gz
| | Sample_series_id | GSE18599
| | Sample_series_id | GSE18600
| | Sample_data_row_count | 45101
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