Search results for the GEO ID: GSE18608  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM462608 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-6A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-6A
|
| Sample_geo_accession | GSM462608
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462608/suppl/GSM462608.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462609 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-6B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-6B
|
| Sample_geo_accession | GSM462609
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462609/suppl/GSM462609.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462610 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-7A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-7A
|
| Sample_geo_accession | GSM462610
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462610/suppl/GSM462610.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462611 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-7B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-7B
|
| Sample_geo_accession | GSM462611
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462611/suppl/GSM462611.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462612 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-8A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-8A
|
| Sample_geo_accession | GSM462612
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462612/suppl/GSM462612.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462613 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-8B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-8B
|
| Sample_geo_accession | GSM462613
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462613/suppl/GSM462613.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462614 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-9A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-9A
|
| Sample_geo_accession | GSM462614
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462614/suppl/GSM462614.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462615 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-9B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-9B
|
| Sample_geo_accession | GSM462615
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462615/suppl/GSM462615.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462616 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-10A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-10A
|
| Sample_geo_accession | GSM462616
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462616/suppl/GSM462616.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462617 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-10B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-10B
|
| Sample_geo_accession | GSM462617
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462617/suppl/GSM462617.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462618 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-17A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-17A
|
| Sample_geo_accession | GSM462618
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462618/suppl/GSM462618.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462619 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-17B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-17B
|
| Sample_geo_accession | GSM462619
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462619/suppl/GSM462619.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462620 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-22A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-22A
|
| Sample_geo_accession | GSM462620
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462620/suppl/GSM462620.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462621 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-22B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-22B
|
| Sample_geo_accession | GSM462621
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462621/suppl/GSM462621.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462622 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-25A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-25A
|
| Sample_geo_accession | GSM462622
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462622/suppl/GSM462622.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462623 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-25B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-25B
|
| Sample_geo_accession | GSM462623
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462623/suppl/GSM462623.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462624 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-43A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-43A
|
| Sample_geo_accession | GSM462624
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462624/suppl/GSM462624.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462625 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-43B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-43B
|
| Sample_geo_accession | GSM462625
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462625/suppl/GSM462625.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462626 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-44A
|
CAD patient, baseline
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: baseline
|
CD133+ cells sorted from PB.
SG-44A
|
| Sample_geo_accession | GSM462626
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462626/suppl/GSM462626.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462627 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-44B
|
CAD patient, after 3 months of exercise
|
tissue: peripheral blood
cell type: CD133+ cells
disease: coronary artery disease
treatment: 3 months of exercise
|
CD133+ cells sorted from PB.
SG-44B
|
| Sample_geo_accession | GSM462627
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462627/suppl/GSM462627.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462628 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-24H
|
healthy subject
|
tissue: peripheral blood
cell type: CD133+ cells
disease: healthy
|
CD133+ cells sorted from PB.
SG-24H
|
| Sample_geo_accession | GSM462628
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462628/suppl/GSM462628.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462629 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-25H
|
healthy subject
|
tissue: peripheral blood
cell type: CD133+ cells
disease: healthy
|
CD133+ cells sorted from PB.
SG-25H
|
| Sample_geo_accession | GSM462629
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462629/suppl/GSM462629.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462630 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-42H
|
healthy subject
|
tissue: peripheral blood
cell type: CD133+ cells
disease: healthy
|
CD133+ cells sorted from PB.
SG-42H
|
| Sample_geo_accession | GSM462630
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462630/suppl/GSM462630.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
GSM462631 | GPL570 |
|
transcriptional profiling of CD133+ cells in coronary artery disease, SG-43H
|
healthy subject
|
tissue: peripheral blood
cell type: CD133+ cells
disease: healthy
|
CD133+ cells sorted from PB.
SG-43H
|
| Sample_geo_accession | GSM462631
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Oct 16 2009
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cardivovascular examination and baseline testing were performed after an overnight fast (except for water). Symptom-limited treadmill exercise was performed using the modified Bruce protocal. Healthy subjects of similar age and sex distribution were recruited for the study to serve as controls for baseline analysis.
| | Sample_growth_protocol_ch1 | CAD patients enrolled in a cardiac rehabilitation program consisting of 36 sessions of 60 minutes each, spaced over 3 months. Medical management was stable for at least a month prior to program participation and all patients were maintained on medications throughout the study as prescribed without change in preparation or dose.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 32 ml of PB was used for isolation of CD133+ cells using fluorescence-actived cell sorting. Total RNA was extracted from CD133+ cells with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
| | Sample_scan_protocol | Affymetrix GeneChip scanner.
| | Sample_data_processing | Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation ('S10'). 26,123 probesets present in at least 2 out of 14 chips (4 healthy controls and 10 CAD baselines) were S10 transformed and analyzed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Delong,,Liu
| | Sample_contact_email | liud2@mail.nih.gov
| | Sample_contact_phone | 301-402-0505
| | Sample_contact_institute | CIT/NIH
| | Sample_contact_address | 15 South Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462631/suppl/GSM462631.CEL.gz
| | Sample_series_id | GSE18608
| | Sample_data_row_count | 26123
| | |
|
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| |
Make groups for comparisons |
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