Search results for the GEO ID: GSE18617  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM462796 | GPL1261 |
|
Bergmann glial cell at age P6, biological replicate 1
|
GFAP-GFP Mouse cerebellum, age P6
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P6
|
Gene expression data from single Bergmann glial cell from P6 mouse cerebellum
|
| Sample_geo_accession | GSM462796
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462796/suppl/GSM462796.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462797 | GPL1261 |
|
Bergmann glial cell at age P6, biological replicate 2
|
GFAP-GFP Mouse cerebellum, age P6
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P6
|
Gene expression data from single Bergmann glial cell from P6 mouse cerebellum
|
| Sample_geo_accession | GSM462797
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462797/suppl/GSM462797.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462798 | GPL1261 |
|
Bergmann glial cell at age P6, biological replicate 3
|
GFAP-GFP Mouse cerebellum, age P6
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P6
|
Gene expression data from single Bergmann glial cell from P6 mouse cerebellum
|
| Sample_geo_accession | GSM462798
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462798/suppl/GSM462798.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462799 | GPL1261 |
|
Bergmann glial cell at age P6, biological replicate 4
|
GFAP-GFP Mouse cerebellum, age P6
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P6
|
Gene expression data from single Bergmann glial cell from P6 mouse cerebellum
|
| Sample_geo_accession | GSM462799
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462799/suppl/GSM462799.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462800 | GPL1261 |
|
Bergmann glial cell at age P6, biological replicate 5
|
GFAP-GFP Mouse cerebellum, age P6
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P6
|
Gene expression data from single Bergmann glial cell from P6 mouse cerebellum
|
| Sample_geo_accession | GSM462800
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462800/suppl/GSM462800.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462801 | GPL1261 |
|
Bergmann glial cell at age P30, biological replicate 1
|
GFAP-GFP Mouse cerebellum, age P30
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P30
|
Gene expression data from single Bergmann glial cell from P30 mouse cerebellum
|
| Sample_geo_accession | GSM462801
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462801/suppl/GSM462801.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462802 | GPL1261 |
|
Bergmann glial cell at age P30, biological replicate 2
|
GFAP-GFP Mouse cerebellum, age P30
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P30
|
Gene expression data from single Bergmann glial cell from P30 mouse cerebellum
|
| Sample_geo_accession | GSM462802
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462802/suppl/GSM462802.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462803 | GPL1261 |
|
Bergmann glial cell at age P30, biological replicate 3
|
GFAP-GFP Mouse cerebellum, age P30
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P30
|
Gene expression data from single Bergmann glial cell from P30 mouse cerebellum
|
| Sample_geo_accession | GSM462803
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462803/suppl/GSM462803.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462804 | GPL1261 |
|
Bergmann glial cell at age P30, biological replicate 4
|
GFAP-GFP Mouse cerebellum, age P30
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P30
|
Gene expression data from single Bergmann glial cell from P30 mouse cerebellum
|
| Sample_geo_accession | GSM462804
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462804/suppl/GSM462804.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
GSM462805 | GPL1261 |
|
Bergmann glial cell at age P30, biological replicate 5
|
GFAP-GFP Mouse cerebellum, age P30
|
tissue: mid-sagittal portion of cerebellum
genotype: GFAP-GFP reporter line
age: P30
|
Gene expression data from single Bergmann glial cell from P30 mouse cerebellum
|
| Sample_geo_accession | GSM462805
| | Sample_status | Public on Feb 12 2010
| | Sample_submission_date | Oct 19 2009
| | Sample_last_update_date | Feb 09 2010
| | Sample_type | other
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | other
| | Sample_extract_protocol_ch1 | Addition of lysis buffer directly to single cell, reverse transcription and PCR based amplification of single cell cDNA was performed as described in previous studies (Tietjen et al, 2003, Neuron; Dulac and Axel, 1995, Cell)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated single-cell cDNA was prepared as described before (Tietjen et al, 2003, Neuron)
| | Sample_hyb_protocol | Following fragmentation, 10-15 ug of cDNA were hybridized on Mouse Gene 430 2.0 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using a Affymetrix GC 3000 high density scanner
| | Sample_data_processing | Analyses of individual microarrays and comparisons between P6 and P30 were performed using GeneSpring GX 7.3 (Agilent). Raw CEL files were processed using the RMA (Robust Multichip Average) normalization algorithm as implemented in GeneSpringGX 7.3. Normalization was performed using default settings, which included data transformation (RAW values of less than 0.01 were set to 0.01), per chip normalization to the median (each measurement was divided by the 50th percentile of all measurements in that sample), and per gene normalization (the raw expression level of each gene was divided by the median of its measurements in all samples). Raw and normalized signal values as well as Present or Absent calls generated using GeneSpring are given in the Sample data table.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,,Corfas
| | Sample_contact_department | Neurobiology Program
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462805/suppl/GSM462805.CEL.gz
| | Sample_series_id | GSE18617
| | Sample_data_row_count | 45101
| | |
|
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