Search results for the GEO ID: GSE18618 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM462810 | GPL570 |
|
NPC 1
|
ReNCell
|
cell type: neural progenitor cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462810
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462810/suppl/GSM462810.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462811 | GPL570 |
|
NPC 2
|
ReNCell
|
cell type: neural progenitor cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462811
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462811/suppl/GSM462811.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462812 | GPL570 |
|
NPC 3
|
ReNCell
|
cell type: neural progenitor cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462812
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462812/suppl/GSM462812.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462813 | GPL570 |
|
iPS Clone 1_1
|
hiPSC footprint-free clone 1
|
cell type: Human induced pluripotent stem cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462813
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462813/suppl/GSM462813.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462814 | GPL570 |
|
iPS Clone 1_2
|
hiPSC footprint-free clone 1
|
cell type: Human induced pluripotent stem cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462814
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462814/suppl/GSM462814.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462815 | GPL570 |
|
iPS Clone 1_3
|
hiPSC footprint-free clone 1
|
cell type: Human induced pluripotent stem cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462815
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462815/suppl/GSM462815.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462816 | GPL570 |
|
iPS Clone 2_1
|
hiPSC footprint-free clone 2
|
cell type: Human induced pluripotent stem cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462816
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462816/suppl/GSM462816.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462817 | GPL570 |
|
iPS Clone 2_2
|
hiPSC footprint-free clone 2
|
cell type: Human induced pluripotent stem cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462817
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462817/suppl/GSM462817.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462818 | GPL570 |
|
iPS Clone 2_3
|
hiPSC footprint-free clone 2
|
cell type: Human induced pluripotent stem cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462818
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462818/suppl/GSM462818.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462819 | GPL570 |
|
HUES6 ES_1
|
HUES6 ES
|
cell type: Human Embryonic Stem Cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462819
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462819/suppl/GSM462819.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462820 | GPL570 |
|
HUES6 ES_2
|
HUES6 ES
|
cell type: Human Embryonic Stem Cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462820
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462820/suppl/GSM462820.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM462821 | GPL570 |
|
HUES6 ES_3
|
HUES6 ES
|
cell type: Human Embryonic Stem Cells
gender: Female
|
Gene expression data
|
Sample_geo_accession | GSM462821
| Sample_status | Public on Oct 20 2009
| Sample_submission_date | Oct 19 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462821/suppl/GSM462821.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM482072 | GPL570 |
|
Cythera ES 1
|
Cythera ES
|
cell type: Human Embryonic Stem Cells
|
Gene expression data
|
Sample_geo_accession | GSM482072
| Sample_status | Public on Dec 09 2009
| Sample_submission_date | Dec 09 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482072/suppl/GSM482072.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM482073 | GPL570 |
|
Cythera ES 2
|
Cythera ES
|
cell type: Human Embryonic Stem Cells
|
Gene expression data
|
Sample_geo_accession | GSM482073
| Sample_status | Public on Dec 09 2009
| Sample_submission_date | Dec 09 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482073/suppl/GSM482073.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
GSM482074 | GPL570 |
|
Cythera ES 3
|
Cythera ES
|
cell type: Human Embryonic Stem Cells
|
Gene expression data
|
Sample_geo_accession | GSM482074
| Sample_status | Public on Dec 09 2009
| Sample_submission_date | Dec 09 2009
| Sample_last_update_date | Dec 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were collected at 50% confluency. For hESC and hiPSC, we first removed any differentiated cell on the plate by manual microdissociation based on morphology.
| Sample_growth_protocol_ch1 | Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin-coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer’s instructions. Human ESC and iPSC were grwon in feeder-free conditions, on matrigel-coated dishes (BD) using mTeSRTM1 .
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was extracted from ~5x106 cells using the RNeasy Protect Mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions, and reverse transcribed using the SuperScript III First-Strand Synthesis System RT-PCR from Invitrogen. The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | according to Affymetrix protocol
| Sample_hyb_protocol | according to Affymetrix protocol
| Sample_scan_protocol | according to Affymetrix protocol
| Sample_data_processing | Human Gene U133_2 arrays were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
| Sample_platform_id | GPL570
| Sample_contact_name | Alysson,R,Muotri
| Sample_contact_email | muotri@ucsd.edu
| Sample_contact_phone | 858-534-9320
| Sample_contact_fax | 858-534-9320
| Sample_contact_department | Pediatrics/CMM
| Sample_contact_institute | University of California San Diego
| Sample_contact_address | 9500 Gilman Drive
| Sample_contact_city | La Jolla
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 92093-0695
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM482nnn/GSM482074/suppl/GSM482074.CEL.gz
| Sample_series_id | GSE18618
| Sample_data_row_count | 54675
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
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