Result

Search results for the GEO ID: GSE18625

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM462902

GPL570

miR-145.1

DLD-1 miR-145

cell type: colon cancer cell line cell line: DLD-1 genome/variation: miR-145 transfectant

BRIC-miR-145-1-U133_Plus_2-a1.CEL

Sample_geo_accession	GSM462902
Sample_status	Public on Oct 01 2010
Sample_submission_date	Oct 19 2009
Sample_last_update_date	Oct 19 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Transfected with Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Sample_growth_protocol_ch1	DLD-1 cells were cultured in DMEM GlutaMAX™-I high glucose medium (31966, Gibco Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS, CH30160.03, Hyclone), 50 U/ml penicillin and 50 µg/ml streptomycin (P/S, 15140 Gibco Invitrogen) and incubated in 5% CO2 plus 20% oxygen.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with TRIZOL 24.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
Sample_hyb_protocol	After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
Sample_data_processing	The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
Sample_platform_id	GPL570
Sample_contact_name	Lea,Haarup,Gregersen
Sample_contact_department	BRIC
Sample_contact_institute	University of Copenhagen
Sample_contact_address	Ole Maaloes vej 5
Sample_contact_city	Coepnhagen N
Sample_contact_zip/postal_code	2200
Sample_contact_country	Denmark
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462902/suppl/GSM462902.CEL.gz
Sample_series_id	GSE18625
Sample_data_row_count	54675

GSM462903

GPL570

miR-145.2

DLD-1 miR-145

cell type: colon cancer cell line cell line: DLD-1 genome/variation: miR-145 transfectant

BRIC-miR-145-2-U133_Plus_2-a1.CEL

Sample_geo_accession	GSM462903
Sample_status	Public on Oct 01 2010
Sample_submission_date	Oct 19 2009
Sample_last_update_date	Oct 19 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Transfected with Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Sample_growth_protocol_ch1	DLD-1 cells were cultured in DMEM GlutaMAX™-I high glucose medium (31966, Gibco Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS, CH30160.03, Hyclone), 50 U/ml penicillin and 50 µg/ml streptomycin (P/S, 15140 Gibco Invitrogen) and incubated in 5% CO2 plus 20% oxygen.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with TRIZOL 24.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
Sample_hyb_protocol	After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
Sample_data_processing	The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
Sample_platform_id	GPL570
Sample_contact_name	Lea,Haarup,Gregersen
Sample_contact_department	BRIC
Sample_contact_institute	University of Copenhagen
Sample_contact_address	Ole Maaloes vej 5
Sample_contact_city	Coepnhagen N
Sample_contact_zip/postal_code	2200
Sample_contact_country	Denmark
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462903/suppl/GSM462903.CEL.gz
Sample_series_id	GSE18625
Sample_data_row_count	54675

GSM462904

GPL570

miR-145.3

DLD-1 miR-145

cell type: colon cancer cell line cell line: DLD-1 genome/variation: miR-145 transfectant

BRIC-miR-145-3-U133_Plus_2-a1.CEL

Sample_geo_accession	GSM462904
Sample_status	Public on Oct 01 2010
Sample_submission_date	Oct 19 2009
Sample_last_update_date	Oct 19 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Transfected with Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Sample_growth_protocol_ch1	DLD-1 cells were cultured in DMEM GlutaMAX™-I high glucose medium (31966, Gibco Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS, CH30160.03, Hyclone), 50 U/ml penicillin and 50 µg/ml streptomycin (P/S, 15140 Gibco Invitrogen) and incubated in 5% CO2 plus 20% oxygen.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with TRIZOL 24.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
Sample_hyb_protocol	After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
Sample_data_processing	The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
Sample_platform_id	GPL570
Sample_contact_name	Lea,Haarup,Gregersen
Sample_contact_department	BRIC
Sample_contact_institute	University of Copenhagen
Sample_contact_address	Ole Maaloes vej 5
Sample_contact_city	Coepnhagen N
Sample_contact_zip/postal_code	2200
Sample_contact_country	Denmark
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462904/suppl/GSM462904.CEL.gz
Sample_series_id	GSE18625
Sample_data_row_count	54675

GSM462905

GPL570

miR-145.4

DLD-1 miR-145

cell type: colon cancer cell line cell line: DLD-1 genome/variation: miR-145 transfectant

BRIC-miR-145-4-U133_Plus_2-a1.CEL

Sample_geo_accession	GSM462905
Sample_status	Public on Oct 01 2010
Sample_submission_date	Oct 19 2009
Sample_last_update_date	Oct 19 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Transfected with Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Sample_growth_protocol_ch1	DLD-1 cells were cultured in DMEM GlutaMAX™-I high glucose medium (31966, Gibco Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS, CH30160.03, Hyclone), 50 U/ml penicillin and 50 µg/ml streptomycin (P/S, 15140 Gibco Invitrogen) and incubated in 5% CO2 plus 20% oxygen.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with TRIZOL 24.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
Sample_hyb_protocol	After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
Sample_data_processing	The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
Sample_platform_id	GPL570
Sample_contact_name	Lea,Haarup,Gregersen
Sample_contact_department	BRIC
Sample_contact_institute	University of Copenhagen
Sample_contact_address	Ole Maaloes vej 5
Sample_contact_city	Coepnhagen N
Sample_contact_zip/postal_code	2200
Sample_contact_country	Denmark
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462905/suppl/GSM462905.CEL.gz
Sample_series_id	GSE18625
Sample_data_row_count	54675

GSM462906

GPL570

mock.1

DLD-1 mock

cell type: colon cancer cell line cell line: DLD-1 genome/variation: mock transfectant

BRIC-mock-1-U133_Plus_2-a1.CEL

Sample_geo_accession	GSM462906
Sample_status	Public on Oct 01 2010
Sample_submission_date	Oct 19 2009
Sample_last_update_date	Oct 19 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Transfected with Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Sample_growth_protocol_ch1	DLD-1 cells were cultured in DMEM GlutaMAX™-I high glucose medium (31966, Gibco Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS, CH30160.03, Hyclone), 50 U/ml penicillin and 50 µg/ml streptomycin (P/S, 15140 Gibco Invitrogen) and incubated in 5% CO2 plus 20% oxygen.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with TRIZOL 24.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
Sample_hyb_protocol	After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
Sample_data_processing	The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
Sample_platform_id	GPL570
Sample_contact_name	Lea,Haarup,Gregersen
Sample_contact_department	BRIC
Sample_contact_institute	University of Copenhagen
Sample_contact_address	Ole Maaloes vej 5
Sample_contact_city	Coepnhagen N
Sample_contact_zip/postal_code	2200
Sample_contact_country	Denmark
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462906/suppl/GSM462906.CEL.gz
Sample_series_id	GSE18625
Sample_data_row_count	54675

GSM462907

GPL570

mock.2

DLD-1 mock

cell type: colon cancer cell line cell line: DLD-1 genome/variation: mock transfectant

BRIC-mock-2-U133_Plus_2-a1.CEL

Sample_geo_accession	GSM462907
Sample_status	Public on Oct 01 2010
Sample_submission_date	Oct 19 2009
Sample_last_update_date	Oct 19 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Transfected with Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Sample_growth_protocol_ch1	DLD-1 cells were cultured in DMEM GlutaMAX™-I high glucose medium (31966, Gibco Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS, CH30160.03, Hyclone), 50 U/ml penicillin and 50 µg/ml streptomycin (P/S, 15140 Gibco Invitrogen) and incubated in 5% CO2 plus 20% oxygen.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with TRIZOL 24.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
Sample_hyb_protocol	After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
Sample_data_processing	The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
Sample_platform_id	GPL570
Sample_contact_name	Lea,Haarup,Gregersen
Sample_contact_department	BRIC
Sample_contact_institute	University of Copenhagen
Sample_contact_address	Ole Maaloes vej 5
Sample_contact_city	Coepnhagen N
Sample_contact_zip/postal_code	2200
Sample_contact_country	Denmark
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462907/suppl/GSM462907.CEL.gz
Sample_series_id	GSE18625
Sample_data_row_count	54675

GSM462908

GPL570

mock.3

DLD-1 mock

cell type: colon cancer cell line cell line: DLD-1 genome/variation: mock transfectant

BRIC-mock-3-U133_Plus_2-a1.CEL

Sample_geo_accession	GSM462908
Sample_status	Public on Oct 01 2010
Sample_submission_date	Oct 19 2009
Sample_last_update_date	Oct 19 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Transfected with Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Sample_growth_protocol_ch1	DLD-1 cells were cultured in DMEM GlutaMAX™-I high glucose medium (31966, Gibco Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS, CH30160.03, Hyclone), 50 U/ml penicillin and 50 µg/ml streptomycin (P/S, 15140 Gibco Invitrogen) and incubated in 5% CO2 plus 20% oxygen.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with TRIZOL 24.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
Sample_hyb_protocol	After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
Sample_data_processing	The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
Sample_platform_id	GPL570
Sample_contact_name	Lea,Haarup,Gregersen
Sample_contact_department	BRIC
Sample_contact_institute	University of Copenhagen
Sample_contact_address	Ole Maaloes vej 5
Sample_contact_city	Coepnhagen N
Sample_contact_zip/postal_code	2200
Sample_contact_country	Denmark
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM462nnn/GSM462908/suppl/GSM462908.CEL.gz
Sample_series_id	GSE18625
Sample_data_row_count	54675

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