Search results for the GEO ID: GSE18636  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM463039 | GPL1261 |
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E9.5 embryo_wild-type_rep1
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Whole embryo, E9.5
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tissue: Cop1 control embryos
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RNA from 6 different control embryos (+/+ or +/-) were mixed and subdivided into control pool 1 and pool 2.
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| Sample_geo_accession | GSM463039
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Oct 20 2009
| | Sample_last_update_date | Jan 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Dr. Sven Bogaerts (Laboratory for Molecular Cancer Biology, VIB-UGent).
| | Sample_treatment_protocol_ch1 | E9.5 Embryos were collected/dissected and immediately lyzed in RNA extraction buffer.
| | Sample_growth_protocol_ch1 | The animals were maintained in accordance with the guidelines of the University of Gent Care and Use ethical Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technolgies) and RNA integrity was assessed using the Bioanalyser 2100 (Agilent).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA normalization. The package affy in Bioconductor was used for this.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463039/suppl/GSM463039.CEL.gz
| | Sample_series_id | GSE18636
| | Sample_data_row_count | 45101
| | |
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GSM463040 | GPL1261 |
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E9.5 embryo_wild-type_rep2
|
Whole embryo, E9.5
|
tissue: Cop1 control embryos
|
RNA from 6 different control embryos (+/+ or +/-) were mixed and subdivided into control pool 1 and pool 2.
|
| Sample_geo_accession | GSM463040
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Oct 20 2009
| | Sample_last_update_date | Jan 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Dr. Sven Bogaerts (Laboratory for Molecular Cancer Biology, VIB-UGent).
| | Sample_treatment_protocol_ch1 | E9.5 Embryos were collected/dissected and immediately lyzed in RNA extraction buffer.
| | Sample_growth_protocol_ch1 | The animals were maintained in accordance with the guidelines of the University of Gent Care and Use ethical Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technolgies) and RNA integrity was assessed using the Bioanalyser 2100 (Agilent).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA normalization. The package affy in Bioconductor was used for this.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463040/suppl/GSM463040.CEL.gz
| | Sample_series_id | GSE18636
| | Sample_data_row_count | 45101
| | |
|
GSM463041 | GPL1261 |
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E9.5 embryo_Cop1KO_rep1
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Whole embryo, E9.5
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tissue: Cop1-null embryos
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RNA from 6 different Cop-null embryos were mixed and subdivided into KO pool 1 and pool 2.
|
| Sample_geo_accession | GSM463041
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Oct 20 2009
| | Sample_last_update_date | Jan 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Dr. Sven Bogaerts (Laboratory for Molecular Cancer Biology, VIB-UGent).
| | Sample_treatment_protocol_ch1 | E9.5 Embryos were collected/dissected and immediately lyzed in RNA extraction buffer.
| | Sample_growth_protocol_ch1 | The animals were maintained in accordance with the guidelines of the University of Gent Care and Use ethical Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technolgies) and RNA integrity was assessed using the Bioanalyser 2100 (Agilent).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA normalization. The package affy in Bioconductor was used for this.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463041/suppl/GSM463041.CEL.gz
| | Sample_series_id | GSE18636
| | Sample_data_row_count | 45101
| | |
|
GSM463042 | GPL1261 |
|
E9.5 embryo_Cop1KO_rep2
|
Whole embryo, E9.5
|
tissue: Cop1-null embryos
|
RNA from 6 different Cop-null embryos were mixed and subdivided into KO pool 1 and pool 2.
|
| Sample_geo_accession | GSM463042
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Oct 20 2009
| | Sample_last_update_date | Jan 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Dr. Sven Bogaerts (Laboratory for Molecular Cancer Biology, VIB-UGent).
| | Sample_treatment_protocol_ch1 | E9.5 Embryos were collected/dissected and immediately lyzed in RNA extraction buffer.
| | Sample_growth_protocol_ch1 | The animals were maintained in accordance with the guidelines of the University of Gent Care and Use ethical Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technolgies) and RNA integrity was assessed using the Bioanalyser 2100 (Agilent).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA normalization. The package affy in Bioconductor was used for this.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463042/suppl/GSM463042.CEL.gz
| | Sample_series_id | GSE18636
| | Sample_data_row_count | 45101
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