Search results for the GEO ID: GSE18680  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM463964 | GPL570 |
|
IGROV1_rep1
|
parental IGROV cell line
|
tissue type: ovarian cancer
cell line: parental IGROV
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463964
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463964/suppl/GSM463964.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463965 | GPL570 |
|
IGROV1_rep2
|
parental IGROV cell line
|
tissue type: ovarian cancer
cell line: parental IGROV
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463965
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463965/suppl/GSM463965.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463966 | GPL570 |
|
IGROV1_rep3
|
parental IGROV cell line
|
tissue type: ovarian cancer
cell line: parental IGROV
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463966
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463966/suppl/GSM463966.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463967 | GPL570 |
|
SKOV3_rep1
|
parental SKOV cell line
|
tissue type: ovarian cancer
cell line: parental SKOV
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463967
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463967/suppl/GSM463967.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463968 | GPL570 |
|
SKOV3_rep2
|
parental SKOV cell line
|
tissue type: ovarian cancer
cell line: parental SKOV
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463968
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463968/suppl/GSM463968.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463969 | GPL570 |
|
SKOV3_rep3
|
parental SKOV cell line
|
tissue type: ovarian cancer
cell line: parental SKOV
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463969
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463969/suppl/GSM463969.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463970 | GPL570 |
|
T112D_rep1
|
parental T112D cell line
|
tissue type: ovarian cancer
cell line: parental T112D
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463970
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463970/suppl/GSM463970.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463971 | GPL570 |
|
T112D_rep2
|
parental T112D cell line
|
tissue type: ovarian cancer
cell line: parental T112D
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463971
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463971/suppl/GSM463971.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463972 | GPL570 |
|
T112D_rep3
|
parental T112D cell line
|
tissue type: ovarian cancer
cell line: parental T112D
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463972
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463972/suppl/GSM463972.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
|
GSM463973 | GPL570 |
|
TOV21G_rep1
|
parental TOV21G cell line
|
tissue type: ovarian cancer
cell line: parental TOV21G
|
parental ovarian cancer cell line
|
| Sample_geo_accession | GSM463973
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Oct 21 2009
| | Sample_last_update_date | Oct 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | The human ovarian cancer cell lines TOV21G, TOV112D and IGROV-1 (from ATCC, Rockville, MD, USA) were cultured in endotoxin-free RPMI 1640 medium supplemented with 3.7 g.l NaHCO3 and 10% FCS. SKOV-3 cellls were cultured in endotoxin-free DMEM medium and 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed and purified further with the Rneasy kit (Qiagen) according to the manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridised for 16 hr at 45C on GeneChip human U133plus2.0 expression array. Genechips were washed and stained in the Affymetrix Fludics Station 450.
| | Sample_scan_protocol | Genechips were scanned using the Affymetrix Genechip Scanner 3000G
| | Sample_data_processing | Data was analysed using Biocondutor 1.95 running on R2.6.0. Probe set expression measures were calcualted using the Affy package Robust Multichip Average default method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hagen,,Kulbe
| | Sample_contact_email | h.kulbe@qmul.ac.uk
| | Sample_contact_department | Centre for Cancer and Inflammation
| | Sample_contact_institute | Institute of Cancer and the CR-UK Clinical Centre, Barts and The London School of Medicine and Dentistry
| | Sample_contact_address | Charterhouse Square
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | EC1M 6BQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM463nnn/GSM463973/suppl/GSM463973.CEL.gz
| | Sample_series_id | GSE18680
| | Sample_data_row_count | 54675
| | |
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