Result

Search results for the GEO ID: GSE18704

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM464422

GPL1261

Granulosa cell with GFP, biological rep1

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express eGFP. cell type: granulosa cell

Control group

Sample_geo_accession	GSM464422
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464422/suppl/GSM464422_Mus_musculus_GFP-1.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464423

GPL1261

Granulosa cell with GFP, biological rep2

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express eGFP. cell type: granulosa cell

Control group

Sample_geo_accession	GSM464423
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464423/suppl/GSM464423_Mus_musculus_GFP-2.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464424

GPL1261

Granulosa cell with GFP, biological rep3

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express eGFP. cell type: granulosa cell

Control group

Sample_geo_accession	GSM464424
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464424/suppl/GSM464424_Mus_musculus_GFP-3.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464425

GPL1261

Granulosa cell with Cre, biological rep1

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express Cre. cell type: granulosa cell

Gene expression data from the effect of Cre

Sample_geo_accession	GSM464425
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464425/suppl/GSM464425_Mus_musculus-Cre-1.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464426

GPL1261

Granulosa cell with Cre, biological rep2

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express Cre. cell type: granulosa cell

Gene expression data from the effect of Cre

Sample_geo_accession	GSM464426
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464426/suppl/GSM464426_Mus_musculus-Cre-2.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464427

GPL1261

Granulosa cell with Cre, biological rep3

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express Cre. cell type: granulosa cell

Gene expression data from the effect of Cre

Sample_geo_accession	GSM464427
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464427/suppl/GSM464427_Mus_musculus_Cre-3.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464428

GPL1261

Granulosa cell with Wnt4, biological rep1

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express Wnt4. cell type: granulosa cell

Gene expression data from the effect of Wnt4

Sample_geo_accession	GSM464428
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464428/suppl/GSM464428_Mus_musculus_Wnt4-1.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464429

GPL1261

Granulosa cell with Wnt4, biological rep2

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express Wnt4. cell type: granulosa cell

Gene expression data from the effect of Wnt4

Sample_geo_accession	GSM464429
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464429/suppl/GSM464429_Mus_musculus-Wnt4-2.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

GSM464430

GPL1261

Granulosa cell with Wnt4, biological rep3

Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment.

treatment protocol: Cells were then infected with adenoviruses to express Wnt4. cell type: granulosa cell

Gene expression data from the effect of Wnt4

Sample_geo_accession	GSM464430
Sample_status	Public on Oct 23 2009
Sample_submission_date	Oct 22 2009
Sample_last_update_date	Oct 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 for 24h in serum-free medium.
Sample_growth_protocol_ch1	Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method. For experiments involving cell culture, cells were suspended in DMEM/F-12 medium (Invitrogen) with 1% fetal bovine serum and allowed to attach overnight at a final density of ~50%.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNeasy mini kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	hybridization was done by the Microarray Core Facility of the Baylor College of Medicine (Houston, TX).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 High-Resolution Scanning Patch.
Sample_data_processing	Microarray data were analyzed using FlexArray 1.3 software (Génome Québec, Montréal, Québec, Canada). Ad-Wnt4, Ad-Cre and control Ad-GFP data were processed using the robust multiarray average algorithm (RMA) for normalization, background correction and expression value calculation.Robustness of the data was further enhanced by the EB (Wright and Simon) algorithm and P-value calculation. A P-value threshold of 0.05 and 3-fold (Ad-Wnt4) or 2-fold (Ad-Cre) change cut-off values were used for identification of differentially expressed genes. Overlap between genes regulated by Ad-Wnt4 and Ad-Cre was also determined using this software.
Sample_platform_id	GPL1261
Sample_contact_name	Derek,,Boerboom
Sample_contact_email	Derek.boerboom@umontreal.ca
Sample_contact_phone	450-773-8521
Sample_contact_fax	450-778-8103
Sample_contact_laboratory	Centre de recherche en reproduction animale
Sample_contact_department	Département de biomédecine Vétérinaire
Sample_contact_institute	Université de Montréal, Faculty of veterinary medicine
Sample_contact_address	3200 Sicotte
Sample_contact_city	Saint-Hyacinthe
Sample_contact_state	QC
Sample_contact_zip/postal_code	J2S2M2
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM464nnn/GSM464430/suppl/GSM464430_Mus_musculus_Wnt4-3.CEL.gz
Sample_series_id	GSE18704
Sample_data_row_count	45101

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