Search results for the GEO ID: GSE18739  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM465435 | GPL570 |
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HEK293 cells in vitro, rep1
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Human embryonic kidney cells (HEK293) in vitro cultivated in DMEM (450 mg/dl glucose)
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cell line: HEK293
cell type: embryonic kidney cells
growth: in vitro cell cultivation
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Gene expression data from in vitro cultivated HEK293 cells.
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| Sample_geo_accession | GSM465435
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Oct 26 2009
| | Sample_last_update_date | Oct 26 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown in DMEM (glucose concentration 450 mg/dl), either injected in NOD.Scid mice and expanded for three weeks in vivo, or cultivated in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer's instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465435/suppl/GSM465435.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465435/suppl/GSM465435.CHP.gz
| | Sample_series_id | GSE18739
| | Sample_data_row_count | 54675
| | |
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GSM465436 | GPL570 |
|
HEK293 cells in vitro, rep2
|
Human embryonic kidney cells (HEK293) in vitro cultivated in DMEM (450 mg/dl glucose)
|
cell line: HEK293
cell type: embryonic kidney cells
growth: in vitro cell cultivation
|
Gene expression data from in vitro cultivated HEK293 cells.
|
| Sample_geo_accession | GSM465436
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Oct 26 2009
| | Sample_last_update_date | Oct 26 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown in DMEM (glucose concentration 450 mg/dl), either injected in NOD.Scid mice and expanded for three weeks in vivo, or cultivated in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer's instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465436/suppl/GSM465436.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465436/suppl/GSM465436.CHP.gz
| | Sample_series_id | GSE18739
| | Sample_data_row_count | 54675
| | |
|
GSM465437 | GPL570 |
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HEK293 in NOD.Scid, rep1
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Human embryonic kidney cells (HEK293) expanded for three weeks in immunodeficient NOD.Scid mice
|
cell line: HEK293
cell type: embryonic kidney cells
growth: in vivo cell expansion
|
Gene expression data from in vivo expanded HEK293 cells.
|
| Sample_geo_accession | GSM465437
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Oct 26 2009
| | Sample_last_update_date | Oct 26 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown in DMEM (glucose concentration 450 mg/dl), either injected in NOD.Scid mice and expanded for three weeks in vivo, or cultivated in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer's instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465437/suppl/GSM465437.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465437/suppl/GSM465437.CHP.gz
| | Sample_series_id | GSE18739
| | Sample_data_row_count | 54675
| | |
|
GSM465438 | GPL570 |
|
HEK293 in NOD.Scid, rep2
|
Human embryonic kidney cells (HEK293) expanded for three weeks in immunodeficient NOD.Scid mice
|
cell line: HEK293
cell type: embryonic kidney cells
growth: in vivo cell expansion
|
Gene expression data from in vivo expanded HEK293 cells.
|
| Sample_geo_accession | GSM465438
| | Sample_status | Public on Oct 27 2009
| | Sample_submission_date | Oct 26 2009
| | Sample_last_update_date | Oct 26 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown in DMEM (glucose concentration 450 mg/dl), either injected in NOD.Scid mice and expanded for three weeks in vivo, or cultivated in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer's instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465438/suppl/GSM465438.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465438/suppl/GSM465438.CHP.gz
| | Sample_series_id | GSE18739
| | Sample_data_row_count | 54675
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