Search results for the GEO ID: GSE18740 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM465440 | GPL1261 |
|
A435_1_Control
|
A435_1_Control
|
agent: Control
|
Gene expression data from BV-2 cells
|
Sample_geo_accession | GSM465440
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465440/suppl/GSM465440_A435_1_Control.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465441 | GPL1261 |
|
A435_2_Control
|
A435_2_Control
|
agent: Control
|
Gene expression data from BV-2 cells
|
Sample_geo_accession | GSM465441
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465441/suppl/GSM465441_A435_2_Control.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465442 | GPL1261 |
|
A435_3_Control
|
A435_3_Control
|
agent: Control
|
Gene expression data from BV-2 cells
|
Sample_geo_accession | GSM465442
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465442/suppl/GSM465442_A435_3_Control.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465443 | GPL1261 |
|
A435_1_Luteolin
|
A435_1_Luteolin
|
agent: Luteolin
|
Gene expression data from BV-2 cells treated with 50 µM Luteolin
|
Sample_geo_accession | GSM465443
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465443/suppl/GSM465443_A435_1_Luteolin.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465444 | GPL1261 |
|
A435_2_Luteolin
|
A435_2_Luteolin
|
agent: Luteolin
|
Gene expression data from BV-2 cells treated with 50 µM Luteolin
|
Sample_geo_accession | GSM465444
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465444/suppl/GSM465444_A435_2_Luteolin.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465445 | GPL1261 |
|
A435_3_Luteolin
|
A435_3_Luteolin
|
agent: Luteolin
|
Gene expression data from BV-2 cells treated with 50 µM Luteolin
|
Sample_geo_accession | GSM465445
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465445/suppl/GSM465445_A435_3_Luteolin.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465446 | GPL1261 |
|
A435_1_LPS
|
A435_1_LPS
|
agent: LPS
|
Gene expression data from BV-2 cells treated with 50 ng/ml LPS
|
Sample_geo_accession | GSM465446
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465446/suppl/GSM465446_A435_1_LPS.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465447 | GPL1261 |
|
A435_2_LPS
|
A435_2_LPS
|
agent: LPS
|
Gene expression data from BV-2 cells treated with 50 ng/ml LPS
|
Sample_geo_accession | GSM465447
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465447/suppl/GSM465447_A435_2_LPS.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465448 | GPL1261 |
|
A435_3_LPS
|
A435_3_LPS
|
agent: LPS
|
Gene expression data from BV-2 cells treated with 50 ng/ml LPS
|
Sample_geo_accession | GSM465448
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465448/suppl/GSM465448_A435_3_LPS.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465449 | GPL1261 |
|
A435_1_LPS_Luteolin
|
A435_1_LPS_Luteolin
|
agent: LPS and luteolin
|
Gene expression data from BV-2 cells treated with 50 ng/ml LPS + 50 µM Luteolin
|
Sample_geo_accession | GSM465449
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465449/suppl/GSM465449_A435_1_LPS_Luteolin.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
|
GSM465450 | GPL1261 |
|
A435_2_LPS_Luteolin
|
A435_2_LPS_Luteolin
|
agent: LPS and luteolin
|
Gene expression data from BV-2 cells treated with 50 ng/ml LPS + 50 µM Luteolin
|
Sample_geo_accession | GSM465450
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465450/suppl/GSM465450_A435_2_LPS_Luteolin.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
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GSM465451 | GPL1261 |
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A435_3_LPS_Luteolin
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A435_3_LPS_Luteolin
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agent: LPS and luteolin
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Gene expression data from BV-2 cells treated with 50 ng/ml LPS + 50 µM Luteolin
|
Sample_geo_accession | GSM465451
| Sample_status | Public on Jan 19 2010
| Sample_submission_date | Oct 26 2009
| Sample_last_update_date | Mar 29 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
| Sample_growth_protocol_ch1 | BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
| Sample_hyb_protocol | The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
| Sample_data_processing | RMA
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,,Langmann
| Sample_contact_email | thomas.langmann@klinik.uni-regensburg.de
| Sample_contact_phone | +49-941-944-5423
| Sample_contact_fax | +49-941-944-5402
| Sample_contact_department | University of Regensburg
| Sample_contact_institute | Institute of Human Genetics
| Sample_contact_address | Franz-Josef-Strauss-Allee 11
| Sample_contact_city | Regensburg
| Sample_contact_zip/postal_code | 93042
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM465nnn/GSM465451/suppl/GSM465451_A435_3_LPS_Luteolin.CEL.gz
| Sample_series_id | GSE18740
| Sample_data_row_count | 45101
| |
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